Effects of the changes in enzyme activities on metabolic flux redistribution around the 2-oxoglutarate branch in glutamate production by Corynebacterium glutamicum

Bioprocess and Biosystems Engineering

Volumn 25, Issue 5, 2003, Pages 291-298

  Shimizu, H ^a   Tanaka, H ^a   Nakato, A ^a   Nagahisa, K ^a   Kimura, E ^b   Shioya, S ^a  

^a OSAKA UNIVERSITY   (Japan)

^b AJINOMOTO CO INC   (Japan)

Author keywords

Flux control of glutamate over production; Metabolic control analysis; Metabolic engineering

Indexed keywords

2 OXOGLUTARIC ACID;

ANALYTIC METHOD; ARTICLE; BIOPROCESS; COMPLEX FORMATION; CORYNEBACTERIUM GLUTAMICUM; ENZYME ACTIVITY; ISOTOPE LABELING; METABOLIC REGULATION; METHODOLOGY; PRIORITY JOURNAL; PROCESS CONTROL; STOICHIOMETRY; TARGET CELL; TISSUE DISTRIBUTION;

CORYNEBACTERIUM; CORYNEBACTERIUM GLUTAMICUM;

EID: 0037350542     PISSN: 16157591     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00449-002-0307-8     Document Type: Article

References (28)

1. Bailey JE (1991) Towards a science of metabolic engineering. Science 252:1668-1675
2. Stephanopoulos G, Vallino JJ (1991) Network rigidity and metabolic engineering in metabolite over production Science 252:1675-1681
3. Vallino JJ, Stephanopoulos G (1993) Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine over production. Biotechnol Bioeng 41:633-646
4. Marx A, de Graaf AA, Wiechert W, Eggeling L, Sahm H (1996) Determination of the fluxes in the central carbon metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol Bioeng 49:111-129
5. Small JR, Kacser H (1993) Response of metabolic systems to large changes in enzyme activities and effectors. 1. The linear treatment of unbranched chains. Europ J Biochem 213:613-624
6. Stephanopoulos G, Simpson T (1997) Flux amplification in complex metabolic network. Chem Eng Sci 52:2607-2627
7. Takiguchi N, Shimizu H, Shioya S (1997) An on-line physiological state recognition system for the lysine fermentation process based on a metabolic reaction model. Biotechnol Bioeng 55:170-181
8. Kawahara Y, Takahashi-Fuke K, Shimizu E, Nakamatsu E, Nakamori S (1997) Relationship between the glutamate production and the activity of 2-oxoglutarate dehydrogenase in Brevibacterium lactofermentum. Biosci Biotechnol Biochem 61:1109-1112
9. Eggeling L, Sham H (1999) L-glutamate and L-lysine: traditional products with impetuous development. Appl Microbiol Biotechnol 52:146-153
10. Stephanopoulos G, Aristidou A, Nielsen J (1998) Metabolic engineering. Academic, San Diego
11. Eikmanns BJ, Rittmann D, Sahm H (1995) Cloning, sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme. J Bacteriol 177:774-782
12. Bormann ER, Eikmanns EJ, Sahm H (1992) Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase. Molecular Microbiol 6:317-326
13. Rollin C, Morgant V, Guyonvarch A, Guerquin-Kern JL (1995) 13C-NMR studies of Corynebacterium melassecola. Europ J Biochem 227:488-493
14. Guillouet S, Lessard PA, Sinskey AJ (2000) Investigating microbial metabolic pathways with NMR: metabolism of amino acids in corynebacteria. In: Barbotin JN, Portais JC (eds) NMR in microbiology: theory and applications. Horizon Scientific, Wymondham, UK, pp 259-281
15. Tesch M, de Graaf AA, Sahm H (1999) In vivo fluxes in the ammonium-assimilatory pathways in Corynebacterium glutamicum studied by 15 N nuclear magnetic resonance. Appl Environ Microbiol 65:1099-1109
16. van der Heijden RTJM, Heijnen JJ, Hellinga C, Romein B, Luyben KCAM (1994) Linear constraint relations in biochemical reaction systems. I. Classification of the calculability and the balanceability of the conversion rates. Biotechnol Bioeng 43:3-10
17. van der Heijden RTJM, Romein B, Heijnen JJ, Hellinga C, Luyben KCAM (1994) Linear constraint relations in biochemical reaction systems. II. Diagnosis and estimation of gross errors. Biotechnol Bioeng 43:11-20
18. Pissara P, Nielsen J, Bazin M (1996) Pathway kinetics and metabolic control analysis. Bioetchnol Bioeng 51:168-176
19. Yang C, Hua Q, Shimizu K (1999) Development of a kinetic model for L-lysine biosynthesis in Corynebacterium glutamicum and its application to metabolic control analysis. J Biosci Bioeng 88:393-403
20. IIGluc of the phosphoenolpyrvate-dependent phosphotranspherase system in Escherichia coli. J Bacteriol 173:6184-6191
21. Small JR, Kacser H (1993) Response of metabolic systems to large changes in enzyme activities and effectors. 2. The linear treatment of branched pathways and metabolite concentrations, assessment of general non-linear case. Europ J Biochem 213:625-640
22. Simpson TW, Shimizu H, Stephanopoulos G (1998) Experimental determination of group flux control coefficients in metabolic networks. Biotechnol Bioeng 58:149-153
23. Shimizu H, Tanaka H, Nakato A, Nagahisa K, Shioya H (2002) Metabolic control analysis in glutamate synthetic pathway. In: Marten M, Park TH, Nagamune T (eds) ACS Symposium Series, Biological systems engineering, vol 830. Oxford University Press, Oxford, pp 39-52
24. Petersen S, Mack C, de Graaf A, Riedel C, Eikmanns BJ, Sahm H (2001) Metabolic consequences of altered phosphoenolpyruvate carboxykinase activity in Corynebacterium glutamicum reveal anaplerotic regulation mechanism in vivo. Metabol Eng 3:344-361
25. Kimura E, Abe C, Kawahara Y, Nakamatsu T, Tokuda H (1997) A dtsR gene-disrupted mutant of Brevibacterium lactofermentum requires fatty acids for growth and efficiently reduces L-glutamate in the presence of an excess of biotin. Biophys Res Comm 234:157-161
26. Kimura E, Yagoshi C, Kawahara Y, Ohsumi T, Nakamatsu T, Tokuda H (1999) Glutamate overproduction in Corynebacterium glutamicum triggered by a decrease in the level of a complex comprising DtsR and a biotin-containing subunit. Biosci Biotechnol Biochem 63:1274-1278
27. Hirasawa T, Wachi M, Nagai K (2000) A mutation in the Corynebacterium glutamicumlts A gene causes susceptibility to lysozyme, temperature-sensitive growth, and L-glutamate production. J Bacteriol 182:2696-2701
28. Loos A, Glanemann C, Willis LB, O'Brien XM, Lessard PA, Gerstmeier R, Guillouet S, Sinskey A (2001) Development and validation of Corynebacterium glutamicum DNA microarrays. Appl Environ Microbiol 67:2310-2318