Improving protein secretion of a transglutaminase-secreting Corynebacterium glutamicum recombinant strain on the basis of 13C metabolic flux analysis

Journal of Bioscience and Bioengineering

Volumn 112, Issue 6, 2011, Pages 595-601

  Umakoshi, Motoki ^a   Hirasawa, Takashi ^a   Furusawa, Chikara ^a   Takenaka, Yasuhiro ^b   Kikuchi, Yoshimi ^b   Shimizu, Hiroshi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b AJINOMOTO CO INC   (Japan)

Author keywords

13C metabolic flux analysis; Batch cultivation; Corynebacterium glutamicum; NADH NAD + ratio; Protein secretion; Transglutaminase

Indexed keywords

BATCH CULTIVATION; CORYNEBACTERIUM GLUTAMICUM; METABOLIC FLUX ANALYSIS; NADH/NAD + RATIO; PROTEIN SECRETION; TRANSGLUTAMINASES;

AMINO ACIDS; CELL CULTURE; GLUCOSE; ISOMERS; METABOLISM; PROTEINS;

PHYSIOLOGY;

CARBON 13; LACTIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL CULTURE; CELL METABOLISM; CITRIC ACID CYCLE; CORYNEBACTERIUM GLUTAMICUM; GLUCOSE TRANSPORT; GLYCOLYSIS; ISOTOPE LABELING; METABOLIC FLUX ANALYSIS; NONHUMAN; PH; PROTEIN SECRETION;

AMINO ACIDS; ANIMALS; CARBON ISOTOPES; CITRIC ACID CYCLE; CORYNEBACTERIUM GLUTAMICUM; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLUCOSE; GLYCOLYSIS; INDUSTRIAL MICROBIOLOGY; MODELS, BIOLOGICAL; TRANSGLUTAMINASES;

CORYNEBACTERIUM GLUTAMICUM;

EID: 83555177335     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.08.011     Document Type: Article

References (25)

1. Kinoshita S., Udaka S., Shimono M. Studies on the amino acid fermentation. Part I. Production of l-glutamic acid by various microorganisms. J. Gen. Appl. Microbiol. 1957, 3:193-205.
2. Nakayama K., Kitada S., Kinoshita S. Studies on lysine fermentation I. The control mechanism of lysine accumulation by homoserine and threonine. J. Gen. Appl. Microbiol. 1961, 7:145-154.
3. Sano K., Shiio I. Microbial production of l-lysine III. Production by mutants resistant to S-(2-aminoethyl)-l-cysteine. J. Gen. Appl. Microbiol. 1970, 16:373-391.
4. Udaka S. Screening method for microorganisms accumulating metabolites and its use in the isolation of Micrococcus glutamicus. J. Bacteriol. 1960, 79:754-755.
5. Kikuchi Y., Date M., Yokoyama K., Umezawa Y., Matsui H. Secretion of active-form Streptoverticillium mobaraense transglutaminase by Corynebacterium glutamicum: processing of the pro-transglutaminase by a cosecreted subtilisin-like protease from Streptomyces albogriseolus. Appl. Environ. Microbiol. 2003, 69:358-366.
6. Yokoyama K., Nio N., Kikuchi Y. Properties and applications of microbial transglutaminase. Appl. Microbiol. Biotechnol. 2004, 64:447-454.
7. Date M., Yokoyama K., Umezawa Y., Matsui H., Kikuchi Y. Production of native-type Streptoverticillium mobaraense transglutaminase in Corynebacterium glutamicum. Appl. Environ. Microbiol. 2003, 69:3011-3014.
8. Stephanopoulos G., Aristidou A.A., Nielsen J. Metabolic engineering: principles and methodologies 1998, Academic Press, San Diego.
9. Iwatani S., Van Dien S., Shimbo K., Kubota K., Kageyama N., Iwahata D., Miyano H., Hirayama K., Usuda Y., Shimizu K., Matsui K. Determination of metabolic flux changes during fed-batch cultivation from measurements of intracellular amino acids by LC-MS/MS. J. Biotechnol. 2007, 128:93-111.
10. 13C labeling experiments. J. Biotechnol. 2007, 129:249-267.
11. 13C labeling data. Biotechnol. Bioeng. 2008, 99:1170-1185.
12. Toya Y., Ishii N., Hirasawa T., Naba M., Hirai K., Sugawara K., Igarashi S., Shimizu K., Tomita M., Soga T. Direct measurement of isotopomer of intracellular metabolites using capillary electrophoresis time-of-flight mass spectrometry for efficient metabolic flux analysis. J. Chromatogr. A 2007, 1159:134-141.
13. 13C-Metabolic flux analysis for batch culture of Escherichia coli and its pyk and pgi gene knockout mutants based on mass isotopomer distribution of intracellular metabolites. Biotechnol. Prog. 2010, 26:975-992.
14. Antoniewicz M.R., Kraynie D.F., Laffend L.A., Gonzalez-Lergier J., Kelleher J.K., Stephanopoulos G. Metabolic flux analysis in a nonstationary system: fed-batch fermentation of a high yielding strain of E. coli producing 1,3-propanediol. Metab. Eng. 2007, 9:277-292.
15. Yokoyama K., Kunio O., Ohtsuka T., Nakamura N., Seguro K., Ejima D. In vitro refolding process of urea-denatured microbial transglutaminase without pro-peptide sequence. Protein Expr. Purif. 2002, 26:329-335.
16. Dauner M., Sauer U. GC-MS analysis of amino acids rapidly provides rich information for isotopomer balancing. Biotechnol. Prog. 2000, 16:642-649.
17. Shirai T., Fujimura K., Furusawa C., Nagahisa K., Shioya S., Shimizu H. Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis. Microb. Cell Fact. 2007, 6:19.
18. Shirai T., Matsuzaki K., Kuzumoto M., Nagahisa K., Furusawa C., Shioya S., Shimizu H. Precise metabolic flux analysis of coryneform bacteria by gas chromatography-mass spectrometry and verification by nuclear magnetic resonance. J. Biosci. Bioeng. 2006, 102:413-424.
19. Laeter J., Bohlke J., Bievre P., Hidaka H., Peiser H., Rosman K., Taylor P. Atomic weights of the elements: review 2000. Pure Appl. Chem. 2003, 75:683-800.
20. van Winden W.A., Wittmann C., Heinzle E., Heijnen J.J. Correcting mass isotopomer distributions for naturally occurring isotopes. Biotechnol. Bioeng. 2002, 80:477-479.
21. Marx A., deGraaf A.A., Wiechert W., Eggeling L., Sahm H. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 1996, 49:111-129.
22. Neidhardt F.C., Ingram J.L., Shchaechter M. Physiology of the bacterial cell 1990, Sinauer Associates, Sunderland.
23. Wiechert W., de Graaf A.A. Bidirectional reaction steps in metabolic networks: I. Modeling and simulation of carbon isotope labeling experiments. Biotechnol. Bioeng. 1997, 55:101-117.
24. Dominguez H., Rollin C., Guyonvarch A., Guerquin-Kern J.L., Cocaign-Bousquet M., Lindley N.D. Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose. Eur. J. Biochem. 1998, 254:96-102.
25. Han S.O., Inui M., Yukawa H. Effect of carbon source availability and growth phase on expression of Corynebacterium glutamicum genes involved in the tricarboxylic acid cycle and glyoxylate bypass. Microbiology 2008, 154:3073-3083.