Hecogenin acetate inhibits reactive oxygen species production and induces cell cycle arrest and senescence in the A549 human lung cancer cell line

Anti-Cancer Agents in Medicinal Chemistry

Volumn 14, Issue 8, 2014, Pages 1128-1135

  Gasparotto, Juciano ^a   Somensi, Nauana ^a   Kunzler, Alice ^a   Girardi, Carolina Saibro ^a   Augusto de Bittencourt Pasquali, Matheus ^a   Ramos, Vitor Miranda ^a   Simões Pires, André ^a   Quintans Júnior, Lucindo José ^b   Branco, Alexsandro ^c   Claudio Fonseca Moreira, José ^a   Gelain, Daniel Pens ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^b FEDERAL UNIVERSITY OF SERGIPE   (Brazil)

^c DEPARTAMENTO DE CIÊNCIAS BIOLÓGICAS   (Brazil)

Author keywords

Cell cycle; ERK1 2; Hecogenin acetate; Non small cell lung cancer; Senescence

Indexed keywords

CATALASE; CISPLATIN; GELATINASE A; HECOGENIN; LACTATE DEHYDROGENASE; MATRIX METALLOPROTEINASE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; REACTIVE OXYGEN METABOLITE; SUPEROXIDE DISMUTASE; WORTMANNIN; ANTINEOPLASTIC AGENT; HECOGENIN ACETATE; MITOGEN ACTIVATED PROTEIN KINASE; SPIRO COMPOUND; STEROID;

ARTICLE; CELL CYCLE ARREST; CELL CYCLE REGULATION; CELL PROLIFERATION; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LUNG CANCER; METASTASIS; MTT ASSAY; PHASE CONTRAST MICROSCOPY; PROTEIN EXPRESSION; TUMOR INVASION; WESTERN BLOTTING; CELL AGING; CELL SURVIVAL; DRUG EFFECTS; G1 PHASE CELL CYCLE CHECKPOINT; LUNG TUMOR; METABOLISM; NON SMALL CELL LUNG CANCER; OXIDATIVE STRESS; PATHOLOGY; PHOSPHORYLATION; TUMOR CELL LINE;

ANTINEOPLASTIC AGENTS; CARCINOMA, NON-SMALL-CELL LUNG; CELL AGING; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; G1 PHASE CELL CYCLE CHECKPOINTS; HUMANS; LUNG NEOPLASMS; MATRIX METALLOPROTEINASE 2; NEOPLASM INVASIVENESS; OXIDATIVE STRESS; PHOSPHORYLATION; REACTIVE OXYGEN SPECIES; SPIRO COMPOUNDS; STEROIDS;

EID: 84907169712     PISSN: 18715206     EISSN: 18755992     Source Type: Journal    
DOI: 10.2174/1871520614666140408151751     Document Type: Article

References (40)

1. Chen, X.; Du, Y.; Nan, J.; Zhang, X.; Qin, X.; Wang, Y.; Hou, J.; Wang, Q.; Yang, J.; Brevilin, A. A novel natural product, inhibits janus kinase activity and blocks STAT3 signaling in cancer cells. PLoS One., 2013, 8(5), 63697.
2. Zhou, J.; Zhao, W.Y.; Ma, X.; Ju, R.J.; Li, X.Y.; Li, N.; Sun, M.G.; Shi, J.F.; Zhang, C.X.; Lu,W.L. The anticancer efficacy of paclitaxel liposomes modified with mitochondrial targeting conjugate in resistant lung cancer. Biomaterials, 2013, 34(14), 3626-3638.
3. Gama, K.B.; Quintans, J.S.; Antoniolli, A.R.; Quintans-Júnior, L.J.; Santana, W.A.; Branco, A.; Soares, M.B.; Villarreal, C.F. Evidence for the involvement of descending pain-inhibitory mechanisms in the antinociceptive effect of hecogenin acetate. J. Nat. Prod., 2013, 76(4), 559-563.
4. Santos Cerqueira, G.; Dos Santose Silva, G.; Rios Vasconcelos, E.; Fragoso De Freitas, A.P.; Arcanjo Moura, B.; Silveira Macedo, D.; Lopes Souto, A.; Barbosa Filho, J.M.; de Almeida Leal, L.K.; de Castro Brito, G.A.; Souccar, C.; De Barros Viana, G.S. Effects of hecogenin and its possible mechanism of action on experimental models of gastric ulcer in mice. Eur. J Pharmacol., 2012, 683(1-3), 260-269.
5. Corbiere, C.; Liagre, B.; Bianchi, A.; Bordji, K.; Dauca, M.; Netter, P.; Beneytout, J.L. Different contribution of apoptosis to the antiproliferative effects of diosgenin and other plant steroids, hecogenin and tigogenin, on human 1547 osteosarcoma cells. Int. J. Oncol., 2003, 22(4), 899-905.
6. Peana, A.T.; Moretti, M.D.; Manconi, V.; Desole, G.; Pippia, P. Anti-inflammatory activity of aqueous extracts and steroidal sapogenins of Agave americana. Planta Med., 1997, 63(3), 199-202.
7. Wang, X.; Liu, J.Z.; Hu, J.X.; Wu, H.; Li, Y.L.; Chen, H.L.; Bai, H.; Hai, C.X. ROS-activated p38 MAPK/ERK-Akt cascade plays a central role in palmitic acid-stimulated hepatocyte proliferation. Free Radic. Biol. Med., 2011, 51(2), 539-551.
8. Kim, Y.M.; Kim, I.H.; Nam, T.J. Inhibition of AGS human gastric cancer cell invasion and proliferation by Capsosiphon fulvescens glycoprotein. Mol. Med. Rep., 2013, 8(1), 11-16.
9. Li, L.; Chen, G.G.; Lu, Y.N.; Liu, Y.; Wu, K.F.; Gong, X.L.; Gou, Z.P.; Li, M.Y.; Liang, N.C. Ent-11α-Hydroxy-15-oxo-kaur-16-en-19-oic-acid inhibits growth of human lung cancer A549 cells by arresting cell cycle and triggering apoptosis. Chin. J. Cancer Res., 2012, 24(2), 109-115.
10. Sabisz, M.; Skladanowski, A. Cancer stem cells and escape from drug-induced premature senescence in human lung tumor cells: implications for drug resistance and in vitro drug screening models. Cell Cycle., 2009, 8(19), 3208-3217.
11. Ho, M.M.; Ng, A.V.; Lam, S.; Hung, J.Y. Side population in human lung cancer cell lines and tumors is enriched with stem-like cancer cells. Cancer Res., 2007, 67(10), 4827-4833.
12. Gelain, D.P.; Moreira, J.C. Evidence of increased reactive species formation by retinol, but not retinoic acid, in PC12 cells. Toxicol In Vitro., 2008, 22(3), 553-558.
13. Ferrari, M.; Fornasiero, M.C.; Isetta, A.M. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J. Immunol. Methods, 1990, 131(2), 165-172.
14. Aebi, H. Catalase in vitro. Methods Enzymol.,1984, 105, 121-126.
15. Misra, H.P.; Fridovich, I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem., 1972, 247(10), 3170-3175.
16. Wang, H.; Joseph, J.A. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic. Biol. Med., 1999, 27(5-6), 612-616.
17. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem., 1976, 72, 248-254.
18. Zanotto-Filho, A.; Delgado-Canedo, A.; Schroder, R.; Becker, M.; Klamt, F.; Moreira, J.C. The pharmacological NFkappaB inhibitors BAY117082 and MG132 induce cell arrest and apoptosis in leukemia cells through ROS-mitochondria pathway activation. Cancer Lett., 2010, 288(2), 192-203.
19. Liagre, B.; Vergne-Salle, P.; Leger, D.Y.; Beneytout, J.L. Inhibition of human rheumatoid arthritis synovial cell survival by hecogenin and tigogenin is associated with increased apoptosis, p38 mitogen-activated protein kinase activity and upregulation of cyclooxygenase-2. Int. J. Mol. Med., 2007, 20(4), 451-460.
20. De Oliveira, M.F.; Amoêdo, N.D.; Rumjanek, F.D. Energy and redox homeostasis in tumor cells. Int. J. Cell Biology., 2012.
21. Benhar, M.; Engelberg, D.; Levitzki, A. ROS, stress-activated kinases and stress signaling in cancer. EMBO Rep., 2002, 3(5), 420-425.
22. Wondrak, G.T. Redox-directed cancer therapeutics: molecular mechanisms and opportunities. Antioxid. Redox Signal., 2009, 11(12), 3013-3069.
23. Cargnello, M.; Roux, P.P. Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases. Microbiol. Mol. Biol. Rev., 2011, 75(1), 50-83.
24. Zhang, W.; Liu, H.T. MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res., 2002, 12(1), 9-18.
25. 2-induced adenocarcinoma gastric cells proliferation via inactivation of MEK1/2-ERK1/2-c-Jun signalling axis. Biochem. Pharmacol., 2009, 77(3), 337-347.
26. 2 cooperatively activated the ERK pathway in selective induction of human urinary bladder cancer J82 cell death. Mol. Carcinog., 2011, 50(3), 215-219.
27. Liagre, B.; Leger, D.Y.; Vergne-Salle, P.; Beneytout, J.L. MAP kinase subtypes and Akt regulate diosgenin-induced apoptosis of rheumatoid synovial cells in association with COX-2 expression and prostanoid production. Int. J. Mol. Med., 2007, 19(1), 113-122.
28. Osmak, M.; Niksíc, D.; Brozović, A.; Ristov, A.A.; Vrhovec, I.; Skrk, J. Drug resistant tumor cells have increased levels of tumor markers for invasion and metastasis. Anticancer Res., 1999, 19(4B), 3193-3197.
29. Mehlen, P.; Puisieux, A. Metastasis: A question of life or death. Nat. Rev. Cancer, 2006, 6(6), 449-458.
30. Liabakk, N.B.; Talbot, I.; Smith, R.A.; Wilkinson, K.; Balkwill, F. Matrix metalloprotease 2 (MMP-2) and matrix metalloprotease 9 (MMP-9) type IV collagenases in colorectal cancer. Cancer Res., 1996, 56(1), 190-196.
31. Wong, C.W.; Lee, A.; Shientag, L.; Yu, J.; Dong, Y.; Kao, G.; Al-Mehdi, A.B.; Bernhard, E.J.; Muschel, R.J. Apoptosis: an early event in metastatic inefficiency. Cancer Res., 2001, 61(1), 333-338.
32. Luzzi, K.J.; MacDonald, I.C.; Schmidt, E.E.; Kerkvliet, N.; Morris, V.L.; Chambers, A.F.; Groom, A.C. Multistep nature of metastatic inefficiency: dormancy of solitary cells after successful extravasation and limited survival of early micrometastases. Am. J. Pathol., 1998, 153(3), 865-873.
33. Wyckoff, J.B.; Jones, J.G.; Condeelis, J.S.; Segall, J.E. A critical step in metastasis: in vivo analysis of intravasation at the primary tumor. Cancer Res., 2000, 60(9), 2504-2511.
34. Reddy, M.V.; Mallireddigari, M.R.; Cosenza, S.C.; Pallela, V.R.; Iqbal, N.M.; Robell, K.A.; Kang, A.D.; Reddy, E.P. Design, synthesis, and biological evaluation of (E)-styrylbenzylsulfones as novel anticancer agents. J. Med. Chem., 2008, 51(1), 86-100.
35. Li, Y.; Ma, H.L.; Han, L.; Liu, W.Y.; Zhao, B.X.; Zhang, S.L.; Miao, J.Y. Novel ferrocenyl derivatives exert anti-cancer effect in human lung cancer cells in vitro via inducing G1-phase arrest and senescence. Acta Pharmacol. Sin., 2013, 34(7), 960-968.
36. Lundholm, L.; Haag, P.; Zong, D.; Juntti, T.; Mork, B.; Lewensohn, R.; Viktorsson, K. Resistance to DNA-damaging treatment in non-small cell lung cancer tumor-initiating cells involves reduced DNA-PK/ATM activation and diminished cell cycle arrest. Cell Death Dis., 2013, 4, 478.
37. Campisi, J. Aging, cellular senescence, and cancer. Annu. Rev. Physiol., 2013, 75, 685-705.
38. Allsopp, R.C.; Chang, E.; Kashefi-Aazam, M.; Rogaev, E.I.; Piatyszek, M.A.; Shay, J.W.; Harley, C.B. Telomere shortening is associated with cell division in vitro and in vivo. Exp. Cell Res., 1995, 220(1), 194-200.
39. Serrano, M.; Lin, A.W.; McCurrach, M.E.; Beach, D.; Lowe, S.W. Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Cell, 1997, 88(5), 593-602.
40. Legakis, J.E.; Koepke, J.I.; Jedeszko, C.; Barlaskar, F.; Terlecky, L.J.; Edwards, H.J.; Walton, P.A.; Terlecky, S.R. Peroxisome senescence in human fibroblasts. Mol. Biol. Cell., 2002, 13(12), 4243-4255.