An intermolecular disulfide bond is required for thermostability and thermoactivity of β-glycosidase from Thermococcus kodakarensis KOD1

Applied Microbiology and Biotechnology

Volumn 98, Issue 18, 2014, Pages 7825-7836

  Hwa, Kuo Yuan ^a   Subramani, Boopathi ^a   Shen, San Tai ^b   Lee, Yu May ^b  

^a NATIONAL TAIPEI UNIVERSITY OF TECHNOLOGY   (Taiwan)

^b INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

Author keywords

Cysteine residues; Disulfide bond; Thermoactivity; Thermostability; glycosidase

Indexed keywords

AMINO ACIDS; COVALENT BONDS; ENZYMES; SULFUR COMPOUNDS;

CYSTEINE RESIDUES; DISULFIDE BONDS; GLYCOSIDASES; THERMOACTIVITY; THERMOSTABILITY;

STABILITY;

BACTERIAL ENZYME; BETA GLYCOSIDASE; CYSTEINE; GLYCOSIDASE; SERINE; UNCLASSIFIED DRUG; DISULFIDE;

BIOTECHNOLOGY; ENZYME ACTIVITY; HOMOLOGY; MOLECULAR ANALYSIS; NUMERICAL MODEL; PROTEIN; SULFIDE; TEMPERATURE EFFECT; THERMOPHILIC BACTERIUM;

AMINO ACID SUBSTITUTION; ARCHAEON; ARTICLE; DISULFIDE BOND; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME STABILITY; FLUORESCENCE SPECTROSCOPY; HALF LIFE TIME; HYDROLYSIS; MOLECULAR CLONING; MOLECULAR MODEL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS; THERMOCOCCUS KODAKARENSIS; CHEMISTRY; ENZYMOLOGY; METABOLISM; TEMPERATURE; THERMOCOCCUS;

THERMOCOCCUS KODAKARENSIS;

DISULFIDES; ENZYME STABILITY; GLYCOSIDE HYDROLASES; MUTAGENESIS, SITE-DIRECTED; TEMPERATURE; THERMOCOCCUS;

EID: 84906944766     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5731-6     Document Type: Article

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