The role of cysteine residues in structure and enzyme activity of a maize β-glucosidase

European Journal of Biochemistry

Volumn 266, Issue 3, 1999, Pages 1056-1065

  Rotrekl, Vladimir ^a,b   Nejedlá, Eliška ^b   Kučera, Igor ^b   Abdallah, Fuad ^c   Palme, Klaus ^c   Brzobohatý, Břetislav ^a,b  

^a INSTITUTE OF BIOPHYSICS   (Czech Republic)

^b MASARYK UNIVERSITY   (Czech Republic)

^c Max Delbruck Laboratorium der Max Planck Gesellschaft   (Germany)

Author keywords

glucosidase; Cysteine residues; Disulfide bridge; Structure fuction relationships

Indexed keywords

BETA GLUCOSIDASE; COMPLEMENTARY DNA; CYSTEINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MAIZE; NONHUMAN; PLANT; PRIORITY JOURNAL; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; BETA-GLUCOSIDASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; ESCHERICHIA COLI; GENES, PLANT; GLUTATHIONE; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; UREA; ZEA MAYS;

EID: 0033572812     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00948.x     Document Type: Article

References (28)

1. 1. Esen, A. (1993) β-Glucosidases: overview. In β-Glucosidases: Biochemistry and Molecular Biology (Esen, A., ed.), pp. 1-14. American Chemical Society, Washington, DC.
2. 2. Brzobohatý, B., Moore, I. & Palme, K. (1994) Cytokinin metabolism: implications for regulation of plant growth and development. Plant Mol. Biol. 26, 1483-1497.
3. 3. Sembdner, G., Atzorn, R. & Schneider, G. (1994) Plant hormone conjugation. Plant Mol. Biol. 26, 1459-1481.
4. 4. Sinnott, M.L. (1990) Catalytic mechanism of enzyme glycosyl transferase. Chem. Rev. 90, 1171-1202.
5. 5. Henrissat, B. & Bairoch, A. (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 293, 781-788.
6. 6. Henrissat, B., Callebaut, I., Fabrega, S., Lehn, P., Mornon, J.-P. & Davies, G. (1995) Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl Acad. Sci. USA 92, 7090-7094.
7. 7. Barret, T., Suresh, C.G., Tolley, S.P., Dodson, E.J. & Hughes, M.A. (1995) The crystal structure of a cyanogenic β-glucosidase from white clover, a family 1 glycosyl hydrolse. Structure 3, 951-960.
8. 8. Jenkins, J., Leggio, L.L., Harris, G. & Pickersgill, R. (1995) β-glucosidase, β-galactosidase, family A cellulases, family F xylanases and two barley glycanases form a superfamily of enzymes with 8-fold β/α architecture and with two conserved glutamates near the carboxy-terminal ends of β-strands four and seven. FEBS Lett. 362, 281-285.
9. 9. Glew, R.H., Peters, S.P. & Christopher, A.R. (1976) Isolation and characterization of β-glucosidase from the cytosol of rat kidney cortex. Biochim. Biophys. Acta 422, 179-199.
10. 10. Falk, A. & Rask, L. (1995) Expression of a zeatin-O-glucoside-degrading β-glucosidase in Brassica napus. Plant Physiol. 108, 1369-1377.
11. 11. Esen, A. (1992) Purification and partitial characterisation of maize (Zea mays L.) β-glucosidase. Plant Physiol. 98, 174-182.
12. 12. Brzobohatý, B., Moore, I., Kristoffersen, P., Bako, L., Campos, N., Schell, J. & Palme, K. (1993) Release of active cytokinin by a β-glucosidase localized to the maize root meristem. Science 262, 1051-1054.
13. 13. Rotrekl, V., Nanak, E., Palme, K. & Brzobohatý, B. (1996) The role of cysteine residues in structure and enzyme activity of a maize β-glucosidase. Chemické Listy. 90, 598-599.
14. 14. Kroll, D.J., Abdel-Hafiz, H.A.-M, Marcell, T., Simpson, S., Chen, C.-Y., Gutierrez-Hartmann, A., Lustbader, J.W. & Hoefler, J.P. (1993) A multifunctional prokaryotic protein expression system: over-production, affinity purification, and selective detection. DNA Cell Biol. 12, 441-453.
15. 15. Zouhar, J., Nanak, E. & Brzobohatý, B. (1999) Expression, single-step purification and matrix-assisted refolding of a maize cytokinin glucoside-specific β-glucosidase. Protein Expression Purif. 17, 153-162.
16. 16. Studier, W., Rosenberg, A.H., Dunn, J.J. & Dubendorff, J.W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185, 1020-1026.
17. 17. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 264-273.
18. 18. Laemli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature 227, 680-685.
19. 19. Towbin, H., Staehelin, T. & Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl Acad. Sci. USA 76, 4350-4354.
20. 20. Blake, M.S., Johnston, K.H., Russel-Jones, G.J. & Gotschlich, E.C. (1984) A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal. Biochem. 136, 175-179.
21. 21. Esen, A. & Cokmus, C. (1990) Maize genotypes classified as null at the glu locus have β-glucosidase activity and immunoreactive protein. Biochem. Genet. 28, 319-336.
22. 22. Jefferson, R.A., Kavangh, T.A. & Bevan, M.W. (1987) GUS fusion: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6, 3901-3907.
23. 23. Ellman, G.L. (1959) Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82, 70-77.
24. 24. Tolley, S.P., Barrett, T.E., Suresh, C.G. & Hughes, M.A. (1993) Crystallization and preliminary crystallographic analysis of the cyanogenic β-glucosidase from white clover Trifolium repens L. J. Mol. Biol. 229, 791-793.
25. 25. Abagyan, R.A., Totrov, M.M. & Kuznetsov, D.N. (1994). ICM - a new method for protein modeling and design. Applications to docking and structure prediction from the distorted native conformation. J. Comp. Chem. 15, 488-506.
26. 26. Abdallah, F. (1999) Entwicklung und Anwendung einer relationalen Makromolekuel-Datenbank. PhD Thesis, University of Cologne, Germany.
27. 27. Nemethy, G., Gibson, K.D., Palmer, K.A., Yoon, C.N., Paterlini, G., Zagari, A., Rumsey, S. & Scheraga, H.A. (1992). Energy Parameters in Polypeptides. 10. Improved geometric parameters and nonbonded interactions for use in the ECEPP/3 algorithm, with application to proline-containing peptides. J. Phys. Chem. 96, 6472-6484.
28. 28. Bairoch, A. & Apweiler, R. (1999) The SWISS-PROT protein sequence data bank and its supplement TrEMBL in 1999. Nucleic Acids Res. 27, 49-54.