Detergent screening of the human voltage-gated proton channel using fluorescence-detection size-exclusion chromatography

Protein Science

Volumn 23, Issue 8, 2014, Pages 1136-1147

  Agharkar, Amruta ^a   Rzadkowolski, Jennifer ^b   McBroom, Mandy ^a   Gonzales, Eric B ^a  

^a UNIVERSITY OF NORTH TEXAS HEALTH SCIENCE CENTER   (United States)

^b University of North Texas Health Science Center   (United States)

Author keywords

Detergent screen; Electrophysiology; Fluorescent detection size exclusion chromatography; Voltage gated proton channel

Indexed keywords

DETERGENT; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HV1 CHANNEL, HUMAN; ION CHANNEL;

ANIMAL; CHEMISTRY; CHO CELL LINE; CRICETULUS; DRUG EFFECTS; FLUORESCENCE; GEL CHROMATOGRAPHY; GENETICS; HUMAN; METABOLISM; SF9 CELL LINE; SOLUBILITY; SPODOPTERA;

ANIMALS; CHO CELLS; CHROMATOGRAPHY, GEL; CRICETULUS; DETERGENTS; FLUORESCENCE; GREEN FLUORESCENT PROTEINS; HUMANS; ION CHANNELS; SF9 CELLS; SOLUBILITY; SPODOPTERA;

EID: 84906886119     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2492     Document Type: Article

References (36)

1. Overington JP, Al-Lazikani B, Hopkins AL (2006) How many drug targets are there? Nat Rev Drug Discov 5: 993-996.
2. Krogh A, Larsson B, von Heijne, G, Sonnhammer EL (2001) Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes. J Mol Biol 305:567-580.
3. Tate CG (2001) Overexpression of mammalian integral membrane proteins for structural studies. FEBS Lett 504:94-98.
4. Bolla JR, Su CC, Yu EW (2012) Biomolecular membrane protein crystallization. Philos Mag 92:2648- 2661.
5. Kawate T, Gouaux E (2006) Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure 14: 673-681.
6. Drew D, Newstead S, Sonoda Y, Kim H, von Heijne, G, Iwata S (2008) GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nat Protoc 3:784-798.
7. Drew D, Kim H (2012) Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins. Methods Mol Biol 866:75-86.
8. DeCoursey TE, Hosler J (2014) Philosophy of voltagegated proton channels. J R Soc Interface 11:20130799.
9. Nawata T, Sibaoka F (1979) Coupling between action potentials and bioluminescence in Noctiluca: effects of inorganic ions and pH in vacuolar sap. J Comparat Physiol 134:137-149.
10. Thomas RC, Meech RW (1982) Hydrogen ion currents and intracellular pH in depolarized voltage-clamped snail neurones. Nature 299:826-828.
11. Eder C, Fischer HG, Hadding U, Heinemann U (1995) Properties of voltage-gated potassium currents of microglia differentiated with granulocyte/macrophage colony-stimulating factor. J Membr Biol 147:137-146.
12. Eder C, Fischer HG, Hadding U, Heinemann U (1995) Properties of voltage-gated currents of microglia developed using macrophage colony-stimulating factor. Pflugers Arch 430:526-533.
13. Sasaki M, Takagi M, Okamura Y (2006) A voltage sensor-domain protein is a voltage-gated proton channel. Science 312:589-592.
14. Ramsey IS, Moran MM, Chong JA, Clapham DE (2006) A voltage-gated proton-selective channel lacking the pore domain. Nature 440:1213-1216.
15. Lee SY, Letts JA, Mackinnon R (2008) Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1. Proc Natl Acad Sci USA 105:7692-7695.
16. Lishko PV, Botchkina IL, Fedorenko A, Kirichok Y (2010) Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel. Cell 140:327-337.
17. Wu LJ, Wu G, Akhavan Sharif MR, Baker A, Jia Y, Fahey FH, Luo HR, Feener EP, Clapham DE (2012) The voltage-gated proton channel Hv1 enhances brain damage from ischemic stroke. Nat Neurosci 15:565-573.
18. Ramsey IS, Ruchti E, Kaczmarek JS, Clapham DE (2009) Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burst. Proc Natl Acad Sci USA 106:7642-7647.
19. Wang Y, Li SJ, Pan J, Che Y, Yin J, Zhao Q (2011) Specific expression of the human voltage-gated proton channel Hv1 in highly metastatic breast cancer cells, promotes tumor progression and metastasis. Biochem Biophys Res Commun 412:353-359.
20. Wang Y, Li SJ, Wu X, Che Y, Li Q (2012) Clinicopathological and biological significance of human voltagegated proton channel Hv1 protein overexpression in breast cancer. J Biol Chem 287:13877-13888.
21. Prive GG (2007) Detergents for the stabilization and crystallization ofmembrane proteins. Methods 41:388-397.
22. Newstead S, Ferrandon S, Iwata S (2008) Rationalizing alpha-helical membrane protein crystallization. Protein Sci 17:466-472.
23. Newstead S, Kim H, von Heijne G, Iwata S, Drew D (2007) High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 104:13936-13941.
24. Li Q, Jogini V, Wanderling S, Cortes DM, Perozo E (2012) Expression, purification, and reconstitution of the voltage-sensing domain from Ci-VSP. Biochemistry 51:8132-8142.
25. Li Q, Wanderling S, Paduch M, Medovoy D, Singharoy A, McGreevy R, Villalba-Galea CA, Hulse RE, Roux B, Schulten K, Kossiakoff A, Perozo E (2014) Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain. Nat Struct Mol Biol 21:244-252.
26. Takeshita K, Sakata S, Yamashita E, Fujiwara Y, Kawanabe A, Kurokawa T, Okochi Y, Matsuda M, Narita H, Okamura Y, Nakagawa A (2014) X-ray crystal structure of voltage-gated proton channel. Nat Struct Mol Biol 21:352-357.
27. Li SJ, Zhao Q, Zhou Q, Unno H, Zhai Y, Sun F (2010) The role and structure of the carboxyl-terminal domain of the human voltage-gated proton channel Hv1. J Biol Chem 285:12047-12054.
28. Chattopadhyay A, London E (1984) Fluorimetric determination of critical micelle concentration avoiding interference from detergent charge. Anal Biochem 139: 408-412.
29. Lee SY, Letts JA, MacKinnon R (2009) Functional reconstitution of purified human Hv1 H1 channels. J Mol Biol 387:1055-1060.
30. Midgett CR, Madden DR (2007) Breaking the bottleneck: eukaryotic membrane protein expression for highresolution structural studies. J Struct Biol 160:265-274.
31. Li SJ, Zhao Q, Zhou Q, Zhai Y (2009) Expression, purification, crystallization and preliminary crystallographic study of the carboxyl-terminal domain of the human voltage-gated proton channel Hv1. Acta Cryst F65:279-281.
32. Fujiwara Y, Kurokawa T, Takeshita K, Kobayashi M, Okochi Y, Nakagawa A, Okamura Y (2012) The cytoplasmic coiled-coil mediates cooperative gating temperature sensitivity in the voltage-gated H(1) channel Hv1. Nat Commun 3:816.
33. Fujiwara Y, Takeshita K, Nakagawa A, Okamura Y (2013) Structural characteristics of the redox-sensing coiled coil in the voltage-gated H1 channel. J Biol Chem 288:17968-17975.
34. Kruse AC, Hu J, Pan AC, Arlow DH, Rosenbaum DM, Rosemond E, Green HF, Liu T, Chae PS, Dror RO, Shaw DE, Weis WI, Wess J, Kobilka BK (2012) Structure and dynamics of the M3 muscarinic acetylcholine receptor. Nature 482:552-556.
35. Gonzales EB, Kawate T, Gouaux E (2009) Pore architecture and ion sites in acid-sensing ion channels and P2X receptors. Nature 460:599-604.
36. Sakata S, Kurokawa T, Norholm MH, Takagi M, Okochi Y, von Heijne G, Okamura Y (2010) Functionality of the voltage-gated proton channel truncated in S4. Proc Natl Acad Sci USA 107:2313-2318.