Expression, purification, and reconstitution of the voltage-sensing domain from Ci-VSP

Biochemistry

Volumn 51, Issue 41, 2012, Pages 8132-8142

  Li, Qufei ^a   Jogini, Vishwanath ^a,b   Wanderling, Sherry ^a   Cortes, D Marien ^a,c   Perozo, Eduardo ^a  

^a UNIVERSITY OF CHICAGO   (United States)

^b D E Shaw Research   (India)

^c TEXAS TECH UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMICAL METHODS; EPR SPECTROSCOPY; FUNCTIONAL BEHAVIORS; HIGH RESOLUTION; MONODISPERSE; PROTEOLIPOSOMES; PROTON CHANNELS; RESTING STATE; ROBUST PROTOCOL; SITE-DIRECTED SPIN LABELING; SPECTROSCOPIC MEASUREMENTS; SPECTROSCOPIC METHOD; STRUCTURAL APPROACH; STRUCTURAL STUDIES; TRANSMEMBRANE SEGMENTS; TRANSMEMBRANE VOLTAGE; VOLTAGE DEPENDENCE; VOLTAGE GATED ION CHANNELS; VOLTAGE SENSING;

ELECTROPHYSIOLOGY; ESCHERICHIA COLI; LIPID BILAYERS; LIPOSOMES; PARAMAGNETIC RESONANCE; PARAMAGNETISM; PROTEINS; SPECTROSCOPIC ANALYSIS; X RAY CRYSTALLOGRAPHY;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

ION CHANNEL; LIPOSOME; PROTEIN CI VSD; PROTEOLIPOSOME; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; CONFORMATIONAL TRANSITION; ELECTRON SPIN RESONANCE; ESCHERICHIA COLI; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; SEQUENCE ALIGNMENT; SPIN LABELING;

AMINO ACID SEQUENCE; ANIMALS; CIONA INTESTINALIS; DNA, COMPLEMENTARY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ION CHANNEL GATING; MOLECULAR SEQUENCE DATA; PHOSPHORIC MONOESTER HYDROLASES; SEQUENCE HOMOLOGY, AMINO ACID; SOLUBILITY;

EID: 84867536704     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300980q     Document Type: Article

References (38)

1. Bezanilla, F. (2008) How membrane proteins sense voltage Nat. Rev. Mol. Cell Biol. 9, 323-332 (Pubitemid 351430853)
2. Swartz, K. J. (2008) Sensing voltage across lipid membranes Nature 456, 891-897
3. Murata, Y., Iwasaki, H., Sasaki, M., Inaba, K., and Okamura, Y. (2005) Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor Nature 435, 1239-1243 (Pubitemid 40943089)
4. Sasaki, M., Takagi, M., and Okamura, Y. (2006) A voltage sensor-domain protein is a voltage-gated proton channel Science 312, 589-592
5. Ramsey, I. S., Moran, M. M., Chong, J. A., and Clapham, D. E. (2006) A voltage-gated proton-selective channel lacking the pore domain Nature 440, 1213-1216
6. Bezanilla, F. (2000) The voltage sensor in voltage-dependent ion channels Physiol. Rev. 80, 555-592 (Pubitemid 30164943)
7. Noda, M., Shimizu, S., Tanabe, T., Takai, T., Kayano, T., Ikeda, T., Takahashi, H., Nakayama, H., Kanaoka, Y., and Minamino, N. 1984, Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence Nature 312, 121-127 (Pubitemid 14000075)
8. Armstrong, C. M. and Bezanilla, F. (1973) Currents related to movement of the gating particles of the sodium channels Nature 242, 459-461
9. Mannuzzu, L. M., Moronne, M. M., and Isacoff, E. Y. (1996) Direct physical measure of conformational rearrangement underlying potassium channel gating Science 271, 213-216 (Pubitemid 26033303)
10. Tempel, B. L., Papazian, D. M., Schwarz, T. L., Jan, Y. N., and Jan, L. Y. (1987) Sequence of a probable potassium channel component encoded at Shaker locus of Drosophila Science 237, 770-775 (Pubitemid 17128270)
11. Bezanilla, F. and Stefani, E. (1998) Gating currents Methods Enzymol. 293, 331-352 (Pubitemid 29342496)
12. Schwarz, T. L., Tempel, B. L., Papazian, D. M., Jan, Y. N., and Jan, L. Y. (1988) Multiple potassium-channel components are produced by alternative splicing at the Shaker locus in Drosophila Nature 331, 137-142 (Pubitemid 18042145)
13. Villalba-Galea, C. A., Sandtner, W., Starace, D. M., and Bezanilla, F. (2008) S4-based voltage sensors have three major conformations Proc. Natl. Acad. Sci. U.S.A. 105, 17600-17607
14. + channel Nature 423, 33-41 (Pubitemid 36569577)
15. Butterwick, J. A. and MacKinnon, R. (2010) Solution structure and phospholipid interactions of the isolated voltage-sensor domain from KvAP J. Mol. Biol. 403, 591-606
16. + channel in a lipid membrane-like environment Nature 450, 376-382 (Pubitemid 350126743)
17. Payandeh, J., Scheuer, T., Zheng, N., and Catterall, W. A. (2011) The crystal structure of a voltage-gated sodium channel Nature 475, 353-358
18. Zhang, X., Ren, W., DeCaen, P., Yan, C., Tao, X., Tang, L., Wang, J., Hasegawa, K., Kumasaka, T., He, J., Clapham, D. E., and Yan, N. (2012) Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel Nature 486, 130-134
19. Tombola, F., Pathak, M. M., Gorostiza, P., and Isacoff, E. Y. (2007) The twisted ion-permeation pathway of a resting voltage-sensing domain Nature 445, 546-549 (Pubitemid 46197635)
20. Starace, D. M. and Bezanilla, F. (2004) A proton pore in a potassium channel voltage sensor reveals a focused electric field Nature 427, 548-553 (Pubitemid 38209113)
21. Sutton, K. A., Jungnickel, M. K., Jovine, L., and Florman, H. M. (2012) Evolution of the Voltage Sensor Domain of the Voltage-Sensitive Phosphoinositide Phosphatase, VSP/TPTE, Suggests a Role as a Proton Channel in Eutherian Mammals Mol. Biol. Evol. 29, 2147-2155
22. Li, Q. and Fung, L. W. (2009) Structural and dynamic study of the tetramerization region of non-erythroid α-spectrin: A frayed helix revealed by site-directed spin labeling electron paramagnetic resonance Biochemistry 48, 206-215
23. Chakrapani, S., Cuello, L. G., Cortes, D. M., and Perozo, E. (2008) Structural dynamics of an isolated voltage-sensor domain in a lipid bilayer Structure 16, 398-409 (Pubitemid 351324110)
24. Vasquez, V., Cortes, D. M., Furukawa, H., and Perozo, E. (2007) An optimized purification and reconstitution method for the MscS channel: Strategies for spectroscopical analysis Biochemistry 46, 6766-6773 (Pubitemid 46906406)
25. + channel in a lipid bilayer Science 306, 491-495 (Pubitemid 39372450)
26. + channel Biochemistry 37, 3229-3236 (Pubitemid 28125548)
27. + channel studied by EPR spectroscopy Nat. Struct. Biol. 5, 459-469 (Pubitemid 28265029)
28. + channel (SKC1): Oligomeric stoichiometry and stability Biochemistry 36, 10343-10352 (Pubitemid 27357747)
29. Killian, J. A. (1998) Hydrophobic mismatch between proteins and lipids in membranes Biochim. Biophys. Acta 1376, 401-415 (Pubitemid 28517889)
30. + channel and its dependence on the lipid membrane Proc. Natl. Acad. Sci. U.S.A. 102, 15441-15446 (Pubitemid 41528076)
31. Milescu, M., Bosmans, F., Lee, S., Alabi, A. A., Kim, J. I., and Swartz, K. J. (2009) Interactions between lipids and voltage sensor paddles detected with tarantula toxins Nat. Struct. Mol. Biol. 16, 1080-1085
32. Schmidt, D., Jiang, Q. X., and MacKinnon, R. (2006) Phospholipids and the origin of cationic gating charges in voltage sensors Nature 444, 775-779 (Pubitemid 44949608)
33. Kohout, S. C., Ulbrich, M. H., Bell, S. C., and Isacoff, E. Y. (2008) Subunit organization and functional transitions in Ci-VSP Nat. Struct. Mol. Biol. 15, 106-108
34. Chakrapani, S., Sompornpisut, P., Intharathep, P., Roux, B., and Perozo, E. (2010) The activated state of a sodium channel voltage sensor in a membrane environment Proc. Natl. Acad. Sci. U.S.A. 107, 5435-5440
35. Vistnes, A. I. and Puskin, J. S. (1981) A spin label method for measuring internal volumes in liposomes or cells, applied to Ca-dependent fusion of negatively charged vesicles Biochim. Biophys. Acta 644, 244-250
36. Chanda, B., Asamoah, O. K., Blunck, R., Roux, B., and Bezanilla, F. (2005) Gating charge displacement in voltage-gated ion channels involves limited transmembrane movement Nature 436, 852-856 (Pubitemid 41160644)
37. + channel Nature 423, 42-48 (Pubitemid 36569578)
38. + channel with a miniature engineered voltage sensor Cell 142, 580-589