Screening for high-yielding saccharomyces cerevisiae clones: Using a green fluorescent protein fusion strategy in the production of membrane proteins

Methods in Molecular Biology

Volumn 866, Issue , 2012, Pages 75-86

  Drew, David ^a   Kim, Hyun ^b  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b Seoul National University   (South Korea)

Author keywords

Fluorescence detection size exclusion chromatography; Membrane protein; Overproduction; S. cerevisiae

Indexed keywords

G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN;

ARTICLE; GEL CHROMATOGRAPHY; GENETICS; METABOLISM; SACCHAROMYCES CEREVISIAE;

CHROMATOGRAPHY, GEL; MEMBRANE PROTEINS; RECEPTORS, G-PROTEIN-COUPLED; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; RATTUS; SACCHAROMYCES CEREVISIAE;

EID: 84859821910     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-770-5_8     Document Type: Article

References (11)

1. Drew DE, von Heijne G, Nordlund P, de Gier JW (2001) Green fl uorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli . FEBS Lett 507:220-224 (Pubitemid 33001546)
2. Drew D, Slotboom DJ, Friso G, Reda T, Genevaux P, Rapp M, Meindl-Beinker NM, Lambert W, Lerch M, Daley DO, Van Wijk KJ, Hirst J, Kunji E, De Gier JW (2005) A scalable GFP-based pipeline for membrane protein overexpression screening and purifi cation. Protein Sci 14:2011-2017 (Pubitemid 41132366)
3. Drew D, Lerch M, Kunji E, Slotboom DJ, de Gier JW (2006) Optimization of membrane protein overexpression and purifi cation using GFP fusions. Nat Methods 3:303-313
4. Drew D, Newstead S, Sonoda Y, Kim H, von Heijne G, Iwata S (2008) GFP-based optimization scheme for the overexpression and purifi cation of eukaryotic membrane proteins in Saccharomyces cerevisiae . Nat Protoc 3:784-798 (Pubitemid 351639023)
5. Sonoda Y, Cameron A, Newstead S, Omote H, Moriyama Y, Kasahara M, Iwata S, Drew D (2010) Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins. FEBS Lett 584:2539-2547
6. Warne T, Serrano-Vega MJ, Tate CG, Schertler GF (2009) Development and crystallization of a minimal thermostabilised G protein-coupled receptor. Protein Expr Purif 65:204-213
7. Bowie JU (2001) Stabilizing membrane proteins. Curr Opin Struct Biol 11:397-402 (Pubitemid 32728488)
8. Newstead S, Kim H, von Heijne G, Iwata S, Drew D (2007) High-throughput fl uorescentbased optimization of eukaryotic membrane protein overexpression and purifi cation in Saccharomyces cerevisiae . Proc Natl Acad Sci USA 104:13936-13941 (Pubitemid 350003316)
9. Kawate T, Gouaux E (2006) Fluorescencedetection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure 14:673-681
10. Iizasa E, Mitsutomi M, Nagano Y (2010) Direct binding of a plant LysM receptor-like kinase, LysM RLK1/CERK1, to chitin in vitro . J Biol Chem 285:2996-3004
11. Sugawara T, Ito K, Shiroishi M, Tokuda N, Asada H, Yurugi-Kobayashi T, Shimamura T, Misaka T, Nomura N, Murata T, Abe K, Iwata S, Kobayashi T (2009) Fluorescence-based optimization of human bitter taste receptor expression in Saccharomyces cerevisiae . Biochem Biophys Res Commun 382:704-710