Rapid 96-well plates DNA extraction and sequencing procedures to identify genome-wide transposon insertion sites in a difficult to lyse bacterium: Lactobacillus casei

Journal of Microbiological Methods

Volumn 106, Issue , 2014, Pages 78-82

  Scornec, Hélène ^a   Tichit, Magali ^b   Bouchier, Christiane ^b   Pédron, Thierry ^b   Cavin, Jean François ^a   Sansonetti, Philippe J ^b,c   Licandro Seraut, Hélène ^a,b  

^a UNIVERSITÉ DE BOURGOGNE   (France)

^b INSTITUT PASTEUR   (France)

^c Unité de Pathogénie Microbienne Moléculaire Institut Pasteur ^*  (France)

Author keywords

Bacterial genetics; DNA extraction; Lactic acid bacteria; Lactobacillus casei; Sanger sequencing; Transposon mutagenesis

Indexed keywords

AMPLICON; ARTICLE; BACTERIAL GENETICS; CONTROLLED STUDY; DNA EXTRACTION; DNA SEQUENCE; GENE IDENTIFICATION; GENE INSERTION; GENE MAPPING; GENETIC ASSOCIATION; LACTOBACILLUS CASEI; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RELIABILITY; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; TRANSPOSON; CHEMISTRY; GENE EXPRESSION REGULATION; GENE LIBRARY; GENETICS; HIGH THROUGHPUT SEQUENCING; ISOLATION AND PURIFICATION; MICROBIAL GENETICS; PROCEDURES; BACTERIAL GENOME; BACTERIAL GROWTH; BACTERIAL STRAIN; DNA LIBRARY; GENE MUTATION; LACTIC ACID BACTERIUM; LYSIS; MUTAGENESIS;

BACTERIAL DNA; TRANSPOSON; GENOMIC DNA;

DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; GENE LIBRARY; GENETICS, MICROBIAL; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; LACTOBACILLUS CASEI; MUTAGENESIS, INSERTIONAL;

EID: 84906768679     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.08.001     Document Type: Article

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