Development of an efficient In vivo system (Pjunc-TpaseIS1223) for random transposon mutagenesis of Lactobacillus casei

Applied and Environmental Microbiology

Volumn 78, Issue 15, 2012, Pages 5417-5423

  Licandro Seraut, Héléne ^a,b   Brinster, Sophie ^c,d   van de Guchte, Maarten ^c   Scornec, Héléne ^a   Maguin, Emmanuelle ^c   Sansonetti, Philippe ^b   Cavin, Jean François ^a   Serror, Pascale ^c  

^a UNIVERSITÉ DE BOURGOGNE   (France)

^b INSTITUT PASTEUR   (France)

^c UMR1319 MICALIS   (France)

^d INSTITUT COCHIN   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPORATION; IN-VIVO; LACTOBACILLUS CASEI; TRANSPOSASE; TRANSPOSON MUTAGENESIS;

BIOTECHNOLOGY; MICROBIOLOGY;

MUTAGENESIS;

PRIMER DNA; TRANSPOSASE;

BACTERIOLOGY; BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; MUTATION; PLASMID;

ARTICLE; ELECTROPORATION; ESCHERICHIA COLI; GENETICS; LACTOBACILLUS CASEI; MUTAGENESIS; PLASMID; SOUTHERN BLOTTING; TRANSPOSON;

BLOTTING, SOUTHERN; DNA PRIMERS; DNA TRANSPOSABLE ELEMENTS; ELECTROPORATION; ESCHERICHIA COLI; LACTOBACILLUS CASEI; MUTAGENESIS; PLASMIDS; TRANSPOSASES;

LACTOBACILLUS CASEI;

EID: 84866174659     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00531-12     Document Type: Article

References (40)

1. Alting-Mees MA, Sorge JA, Short JM. 1992. pBluescriptII: multifunctional cloning and mapping vectors. Methods Enzymol. 216:483-495.
2. Aukrust TW, Brurberg MB, Nes IF. 1995. Transformation of Lactobacillus by electroporation. Methods Mol. Biol. 47:201-208.
3. Berger B, et al. 2007. Similarity and differences in the Lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics. J. Bacteriol. 189:1311-1321.
4. Bhowmik T, Fernandez L, Steele JL. 1993. Gene replacement in Lactobacillus helveticus. J. Bacteriol. 175:6341-6344.
5. Blouzard JC, Valette O, Tardif C, de Philip P. 2010. Random mutagenesis of Clostridium cellulolyticum by using a Tn1545 derivative. Appl. Environ. Microbiol. 76:4546-4549.
6. Brantl S, Kummer C, Behnke D. 1994. Complete nucleotide sequence of plasmid pGB3631, a derivative of the Streptococcus agalactiae plasmid pIP501. Gene 142:155-156.
7. Cai H, Thompson R, Budinich MF, Broadbent JR, Steele JL. 2009. Genome sequence and comparative genome analysis of Lactobacillus casei: insights into their niche-associated evolution. Genome Biol. Evol. 1:239-257.
8. Choi KH, Kim KJ. 2009. Applications of transposon-based gene delivery system in bacteria. J. Microbiol. Biotechnol. 19:217-228.
9. Chouayekh H, Serror P, Boudebbouze S, Maguin E. 2009. Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene. FEMS Microbiol. Lett. 293:232-239.
10. Churchward G, Belin D, Nagamine Y. 1984. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene 31:165-171.
11. Craig NL. 1997. Target site selection in transposition. Annu. Rev. Biochem. 66:437-474.
12. Dower WJ, Miller JF, Ragsdale CW. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16:6127-6145.
13. Duval-Valentin G, Marty-Cointin B, Chandler M. 2004. Requirement of IS911 replication before integration defines a new bacterial transposition pathway. EMBO J. 23:3897-3906.
14. Duval-Valentin G, Normand C, Khemici V, Marty B, Chandler M. 2001. Transient promoter formation: a new feedback mechanism for regulation of IS911 transposition. EMBO J. 20:5802-5811.
15. Evans RP, Jr, Macrina FL. 1983. Streptococcal R plasmid pIP501: endo-nuclease site map, resistance determinant location, and construction of novel derivatives. J. Bacteriol. 154:1347-1355.
16. Gibson TJ. 1984. Studies on the Epstein-Barr virus genome. Ph.D. thesis. Cambridge University, Cambridge, United Kingdom.
17. Goryshin IY, Jendrisak J, Hoffman LM, Meis R, Reznikoff WS. 2000. Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes. Nat. Biotechnol. 18:97-100.
18. Guedon E, Serror P, Ehrlich SD, Renault P, Delorme C. 2001. Pleiotropic transcriptional repressor CodY senses the intracellular pool of branched-chain amino acids in Lactococcus lactis. Mol. Microbiol. 40: 1227-1239.
19. Gury J, Barthelmebs L, Cavin J-F. 2004. Random transposon mutagenesis of Lactobacillus plantarum by using the pGh9:ISS1 vector to clone genes involved in the regulation of phenolic acid metabolism. Arch. Microbiol. 182:337-345.
20. Heap JT, Pennington OJ, Cartman ST, Minton NP. 2009. A modular system for Clostridium shuttle plasmids. J. Microbiol. Methods 78:79-85.
21. Hershfield V. 1979. Plasmids mediating multiple drug resistance in group B Streptococcus: transferability and molecular properties. Plasmid 2:137-149.
22. Horodniceanu T, Bouanchaud DH, Bieth G, Chabbert YA. 1976. Rplasmids in Streptococcus agalactiae (group B). Antimicrob. Agents Chemother. 10:795-801.
23. Ito M, et al. 2010. A practical random mutagenesis system for probiotic Lactobacillus casei using Tn5 transposition complexes. J. Appl. Microbiol. 109:657-666.
24. Kay R, McPherson J. 1987. Hybrid pUC vectors for addition of new restriction enzyme sites to the ends of DNA fragments. Nucleic Acids Res. 15:2778.
25. Licandro-Seraut H, Gury J, Tran NP, Barthelmebs L, Cavin JF. 2008. Kinetics and intensity of the expression of genes involved in the stress response tightly induced by phenolic acids in Lactobacillus plantarum. J. Mol. Microbiol. Biotechnol. 14:41-47.
26. Luchansky JB, Muriana PM, Klaenhammer TR. 1988. Application of electroporation for transfer of plasmid DNA to Lactobacillus, Lactococcus, Leuconostoc, Listeria, Pediococcus, Bacillus, Staphylococcus, Enterococcus and Propionibacterium. Mol. Microbiol. 2:637-646.
27. Maguin E, Prévost H, Ehrlich SD, Gruss A. 1996. Efficient insertional mutagenesis in lactococci and other gram-positive bacteria. J. Bacteriol. 178:931-935.
28. Mahillon J, Chandler M. 1998. Insertion sequences. Microbiol. Mol. Biol. Rev. 62:725-774.
29. Marco ML, Pavan S, Kleerebezem M. 2006. Towards understanding molecular modes of probiotic action. Curr. Opin. Biotechnol. 17:204-210.
30. Pujol C, Chedin F, Ehrlich SD, Janniere L. 1998. Inhibition of a naturally occurring rolling-circle replicon in derivatives of the theta-replicating plasmid pIP501. Mol. Microbiol. 29:709-718.
31. Serror P, Ilami G, Chouayekh H, Ehrlich SD, Maguin E. 2003. Transposition in Lactobacillus delbrueckii subsp. bulgaricus: identification of two thermosensitive replicons and two functional insertion sequences. Microbiology. 149:1503-1511.
32. Serror P, Sasaki T, Ehrlich SD, Maguin E. 2002. Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids. Appl. Environ. Microbiol. 68:46-52.
33. Skulj M, et al. 2008. Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes. Microb. Cell Fact. 7:6. doi:10.1186/1475-2859-7-6.
34. Steinmetz M, Richter R. 1994. Easy cloning of mini-Tn10 insertions from the Bacillus subtilis chromosome. J. Bacteriol. 176:1761-1763.
35. Tien MT, et al. 2006. Anti-inflammatory effect of Lactobacillus casei on Shigella-infected human intestinal epithelial cells. J. Immunol. 176:1228-1237.
36. Tran NP, et al. 2008. Phenolic acid-mediated regulation of the padC gene, encoding the phenolic acid decarboxylase of Bacillus subtilis. J. Bacteriol. 190:3213-3224.
37. Vagner V, Dervyn E, Ehrlich SD. 1998. A vector for systematic gene inactivation in Bacillus subtilis. Microbiology 144(Pt 11):3097-3104.
38. van der Vossen JM, van der Lelie D, Venema G. 1987. Isolation and characterization of Streptococcus cremoris Wg2-specific promoters. Appl. Environ. Microbiol. 53:2452-2457.
39. Walker DC, Klaenhammer TR. 1994. Isolation of a novel IS3 group insertion element and construction of an integration vector for Lactobacillus spp. J. Bacteriol. 176:5330-5340.
40. Zhang ZG, Ye ZQ, Yu L, Shi P. 2011. Phylogenomic reconstruction of lactic acid bacteria: an update. BMC Evol. Biol. 11:1.