Use of a high-throughput screen to identify Leptospira mutants unable to colonize the carrier host or cause disease in the acute model of infection

Journal of Medical Microbiology

Volumn 62, Issue PART10, 2013, Pages 1601-1608

  Marcsisin, Renee A ^a,d   Bartpho, Thanatchaporn ^b   Bulach, Dieter M ^a   Srikram, Amporn ^c   Sermswan, Rasana W ^b   Adler, Ben ^a   Murray, Gerald L ^a  

^a MONASH UNIVERSITY   (Australia)

^b KHON KAEN UNIVERSITY   (Thailand)

^c KASETSART UNIVERSITY   (Thailand)

^d UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

IRON; VIRULENCE FACTOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL COLONIZATION; BACTERIAL VIRULENCE; FEMALE; GENE DELETION; GENE INSERTION; HAMSTER; HIGH THROUGHPUT SCREENING; HOST; IRON TRANSPORT; LEPTOSPIRA; LEPTOSPIROSIS; MUTANT; NONHUMAN; PRIORITY JOURNAL; TRANSPOSON;

ANIMALS; CARRIER STATE; DISEASE MODELS, ANIMAL; DNA TRANSPOSABLE ELEMENTS; FEMALE; GENE KNOCKOUT TECHNIQUES; GENETIC TESTING; HIGH-THROUGHPUT SCREENING ASSAYS; LEPTOSPIRA; LEPTOSPIROSIS; MALE; MICE; MICE, INBRED BALB C; MUTAGENESIS, INSERTIONAL; VIRULENCE FACTORS;

EID: 84884491909     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.058586-0     Document Type: Article

References (37)

1. Adler, B. & de la Peña Moctezuma, A. (2010). Leptospira and leptospirosis. Vet Microbiol 140, 287-296.
2. Adler, B., Lo, M., Seemann, T. & Murray, G. L. (2011). Pathogenesis of leptospirosis: the influence of genomics. Vet Microbiol 153, 73-81.
3. Asuthkar, S., Velineni, S., Stadlmann, J., Altmann, F. & Sritharan, M. (2007). Expression and characterization of an iron-regulated heminbinding protein, HbpA, from Leptospira interrogans serovar Lai. Infect Immun 75, 4582-4591.
4. Bourhy, P., Louvel, H., Saint Girons, I. & Picardeau, M. (2005). Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon. J Bacteriol 187, 3255-3258.
5. Croda, J., Figueira, C. P., Wunder, E. A., Jr, Santos, C. S., Reis, M. G., Ko, A. I. & Picardeau, M. (2008). Targeted mutagenesis in pathogenic Leptospira species: disruption of the LigB gene does not affect virulence in animal models of leptospirosis. Infect Immun 76, 5826-5833.
6. Dang, W., Hu, Y. H. & Sun, L. (2011). HtpG is involved in the pathogenesis of Edwardsiella tarda. Vet Microbiol 152, 394-400.
7. David, M., Dzamba, M., Lister, D., Ilie, L. & Brudno, M. (2011). SHRiMP2: sensitive yet practical SHort Read Mapping. Bioinformatics 27, 1011-1012.
8. 2 and for virulence. Infect Immun 80, 3892-3899.
9. Faine, S. (1962a). Factors affecting the development of the carrier state in leptospirosis. J Hyg (Lond) 60, 427-434.
10. Faine, S. (1962b). The growth of Leptospira australis B in the kidneys of mice in the incipient experimental carrier state. J Hyg (Lond) 60, 435-442.
11. Fluckiger, U., Ulrich, M., Steinhuber, A., Döring, G., Mack, D., Landmann, R., Goerke, C. & Wolz, C. (2005). Biofilm formation, icaADBC transcription, and polysaccharide intercellular adhesin synthesis by staphylococci in a device-related infection model. Infect Immun 73, 1811-1819.
12. Joshi, S. M., Pandey, A. K., Capite, N., Fortune, S. M., Rubin, E. J. & Sassetti, C. M. (2006). Characterization of mycobacterial virulence genes through genetic interaction mapping. Proc Natl Acad Sci U S A 103, 11760-11765.
13. King, A. M., Bartpho, T., Sermswan, R. W., Bulach, D. M., Eshghi, A., Picardeau, M., Adler, B. & Murray, G. L. (2013). Leptospiral outer membrane protein LipL41 is not essential for acute leptospirosis, but requires a small chaperone, Lep, for stable expression. Infect Immun 81, 2768-2776.
14. Lambert, A., Picardeau, M., Haake, D. A., Sermswan, R. W., Srikram, A., Adler, B. & Murray, G. L. (2012). FlaA proteins in Leptospira interrogans are essential for motility and virulence but are not required for formation of the flagellum sheath. Infect Immun 80, 2019-2025.
15. Liao, S., Sun, A., Ojcius, D. M., Wu, S., Zhao, J. & Yan, J. (2009). Inactivation of the fliY gene encoding a flagellar motor switch protein attenuates mobility and virulence of Leptospira interrogans strain Lai. BMC Microbiol 9, 253.
16. Lo, M., Murray, G. L., Khoo, C. A., Haake, D. A., Zuerner, R. L. & Adler, B. (2010). Transcriptional response of Leptospira interrogans to iron limitation and characterization of a PerR homolog. Infect Immun 78, 4850-4859.
17. Logsdon, L. K. & Mecsas, J. (2003). Requirement of the Yersinia pseudotuberculosis effectors YopH and YopE in colonization and persistence in intestinal and lymph tissues. Infect Immun 71, 4595-4607.
18. Lourdault, K., Cerqueira, G. M., Wunder, E. A., Jr & Picardeau, M. (2011). Inactivation of clpB in the pathogen Leptospira interrogans reduces virulence and resistance to stress conditions. Infect Immun 79, 3711-3717.
19. Mérien, F., Amouriaux, P., Perolat, P., Baranton, G. & Saint Girons, I. (1992). Polymerase chain reaction for detection of Leptospira spp. in clinical samples. J Clin Microbiol 30, 2219-2224.
20. Monahan, A. M., Callanan, J. J. & Nally, J. E. (2008). Proteomic analysis of Leptospira interrogans shed in urine of chronically infected hosts. Infect Immun 76, 4952-4958.
21. Murray, G. L., Ellis, K. M., Lo, M. & Adler, B. (2008). Leptospira interrogans requires a functional heme oxygenase to scavenge iron from hemoglobin. Microbes Infect 10, 791-797.
22. Murray, G. L., Morel, V., Cerqueira, G. M., Croda, J., Srikram, A., Henry, R., Ko, A. I., Dellagostin, O. A., Bulach, D. M. & other authors (2009a). Genome-wide transposon mutagenesis in pathogenic Leptospira species. Infect Immun 77, 810-816.
23. Murray, G. L., Srikram, A., Henry, R., Puapairoj, A., Sermswan, R. W. & Adler, B. (2009b). Leptospira interrogans requires heme oxygenase for disease pathogenesis. Microbes Infect 11, 311-314.
24. Murray, G. L., Srikram, A., Hoke, D. E., Wunder, E. A., Jr, Henry, R., Lo, M., Zhang, K., Sermswan, R. W., Ko, A. I. & Adler, B. (2009c). Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans. Infect Immun 77, 952-958.
25. Murray, G. L., Srikram, A., Henry, R., Hartskeerl, R. A., Sermswan, R. W. & Adler, B. (2010). Mutations affecting Leptospira interrogans lipopolysaccharide attenuate virulence. Mol Microbiol 78, 701-709.
26. Nally, J. E., Chow, E., Fishbein, M. C., Blanco, D. R. & Lovett, M. A. (2005). Changes in lipopolysaccharide O antigen distinguish acute versus chronic Leptospira interrogans infections. Infect Immun 73, 3251-3260.
27. Nally, J. E., Monahan, A. M., Miller, I. S., Bonilla-Santiago, R., Souda, P. & Whitelegge, J. P. (2011). Comparative proteomic analysis of differentially expressed proteins in the urine of reservoir hosts of leptospirosis. PLoS ONE 6, e26046.
28. Nascimento, A. L., Ko, A. I., Martins, E. A., Monteiro-Vitorello, C. B., Ho, P. L., Haake, D. A., Verjovski-Almeida, S., Hartskeerl, R. A., Marques, M. V. & other authors (2004). Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis. J Bacteriol 186, 2164-2172.
29. Ren, S. X., Fu, G., Jiang, X. G., Zeng, R., Miao, Y. G., Xu, H., Zhang, Y. X., Xiong, H., Lu, G. & other authors (2003). Unique physiological and pathogenic features of Leptospira interrogans revealed by wholegenome sequencing. Nature 422, 888-893.
30. Ristow, P., Bourhy, P., da Cruz McBride, F. W., Figueira, C. P., Huerre, M., Ave, P., Girons, I. S., Ko, A. I. & Picardeau, M. (2007). The OmpA-like protein Loa22 is essential for leptospiral virulence. PLoS Pathog 3, e97.
31. Santos, C. S., Macedo, J. O., Bandeira, M., Chagas-Junior, A. D., McBride, A. J., McBride, F. W., Reis, M. G. & Athanazio, D. A. (2010). Different outcomes of experimental leptospiral infection in mouse strains with distinct genotypes. J Med Microbiol 59, 1101-1106.
32. Staden, R. (1979). A strategy of DNA sequencing employing computer programs. Nucleic Acids Res 6, 2601-2610.
33. Sweier, D. G., Combs, A., Shelburne, C. E., Fenno, J. C. & Lopatin, D. E. (2003). Construction and characterization of a Porphyromonas gingivalis htpG disruption mutant. FEMS Microbiol Lett 225, 101-106.
34. Terry, K., Williams, S. M., Connolly, L. & Ottemann, K. M. (2005). Chemotaxis plays multiple roles during Helicobacter pylori animal infection. Infect Immun 73, 803-811.
35. Tucunduva de Faria, M., Athanazio, D. A., Gonçalves Ramos, E. A., Silva, E. F., Reis, M. G. & Ko, A. I. (2007). Morphological alterations in the kidney of rats with natural and experimental Leptospira infection. J Comp Pathol 137, 231-238.
36. Vergara-Irigaray, N., Chávarri-Martínez, A., Rodríguez-Cuesta, J., Miller, J. F., Cotter, P. A. & Martínez de Tejada, G. (2005). Evaluation of the role of the Bvg intermediate phase in Bordetella pertussis during experimental respiratory infection. Infect Immun 73, 748-760.
37. Zhang, L., Zhang, C., Ojcius, D. M., Sun, D., Zhao, J., Lin, X., Li, L., Li, L. & Yan, J. (2012). The mammalian cell entry (Mce) protein of pathogenic Leptospira species is responsible for RGD motif-dependent infection of cells and animals. Mol Microbiol 83, 1006-1023.