One-step generation of phenotype-expressing triple-knockout mice with heritable mutated alleles by the CRISPR/Cas9 system

Journal of Reproduction and Development

Volumn 60, Issue 4, 2014, Pages 324-327

  Fujii, Wataru ^a   Onuma, Asuka ^a   Sugiura, Koji ^a   Naito, Kunihiko ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

CRISPR Cas9; Heritable; Mouse; Phenotype; Triple knockout

Indexed keywords

MUS;

ALLELE; ANIMAL; C57BL MOUSE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; FEMALE; GENE INACTIVATION; KNOCKOUT MOUSE; MUTATION; PHENOTYPE;

ALLELES; ANIMALS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; FEMALE; GENE KNOCKOUT TECHNIQUES; MICE, INBRED C57BL; MICE, KNOCKOUT; MUTATION; PHENOTYPE;

EID: 84905757765     PISSN: 09168818     EISSN: 13484400     Source Type: Journal    
DOI: 10.1262/jrd.2013-139     Document Type: Article

References (12)

1. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Multiplex genome engineering using CRISPR/Cas systems. Science 2013; 339: 819-823.
2. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. RNA-guided human genome engineering via Cas9. Science 2013; 339: 823-826.
3. Fujii W, Kawasaki K, Sugiura K, Naito K. Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res 2013; 41: e187.
4. Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 2013; 153: 910-918.
5. Li W, Teng F, Li T, Zhou Q. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat Biotechnol 2013; 31: 684-686.
6. Zhou J, Shen B, Zhang W, Wang J, Yang J, Chen L, Zhang N, Zhu K, Xu J, Hu B, Leng Q, Huang X. One-step generation of different immunodeficient mice with multiple gene modifications by CRISPR/Cas9 mediated genome engineering. Int J Biochem Cell Biol 2014; 46: 49-55.
7. Le Fur N, Kelsall SR, Mintz B. Base substitution at different alternative splice donor sites of the tyrosinase gene in murine albinism. Genomics 1996; 37: 245-248.
8. McPherron AC, Lawler AM, Lee SJ. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature 1997; 387: 83-90.
9. Wu CL, Melton DW. Production of a model for Lesch-Nyhan syndrome in hypoxanthine phosphoribosyltransferase-deficient mice. Nat Genet 1993; 3: 235-240.
10. Yamagata K, Yamazaki T, Yamashita M, Hara Y, Ogonuki N, Ogura A. Noninvasive visualization of molecular events in the mammalian zygote. Genesis 2005; 43: 71-79.
11. Fujii W, Onuma A, Sugiura K, Naito K. Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system. Biochem Biophys Res Commun 2014; 445: 791-794.
12. Iseli C, Ambrosini G, Bucher P, Jongeneel CV. Indexing strategies for rapid searches of short words in genome sequences. PLoS ONE 2007; 2: e579.