An improved method and cost effective strategy for soluble expression and purification of human N-myristoyltransferase 1 in E. coli

Molecular and Cellular Biochemistry

Volumn 392, Issue 1-2, 2014, Pages 175-186

  Kumar, Sujeet ^a   Sharma, Rajendra K ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Auto induction; Myristoylation; N myristoyltransferase (NMT); Protein purification; Soluble expression; T7 expression system

Indexed keywords

ANTINEOPLASTIC AGENT; ISOENZYME; ISOPROPYL BETA DEXTRO 1 THIOGALACTOPYRANOSIDE; N MYRISTOYLTRANSFERASE 1; PROTEIN N MYRISTOYLTRANSFERASE; PYRANOSIDE; UNCLASSIFIED DRUG; ACYLTRANSFERASE; GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE; PRIMER DNA; RECOMBINANT PROTEIN;

ARTICLE; COST EFFECTIVENESS ANALYSIS; DRUG DESIGN; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME PURIFICATION; ESCHERICHIA COLI; HUMAN; LIGAND BINDING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MYRISTYLATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SPECTROFLUOROMETRY; AMINO ACID SEQUENCE; CHEMISTRY; COST BENEFIT ANALYSIS; GENETICS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; BASE SEQUENCE; COST-BENEFIT ANALYSIS; DNA PRIMERS; ESCHERICHIA COLI; HUMANS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE;

EID: 84905191726     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-014-2029-z     Document Type: Article

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