An integrated study of natural hydroxyapatite-induced osteogenic differentiation of mesenchymal stem cells using transcriptomics, proteomics and microRNA analyses

Biomedical Materials (Bristol)

Volumn 9, Issue 4, 2014, Pages

  Zhang, Zhiwei ^a   Wang, Jiandan ^a   Lü, Xiaoying ^a  

^a SOUTHEAST UNIVERSITY   (China)

Author keywords

biological pathways; microRNA; natural hydroxyapatite; osteoinduction; proteomics; transcriptomics

Indexed keywords

CELL CULTURE; CELL PROLIFERATION; FLOWCHARTING; HYDROXYAPATITE; MOLECULAR BIOLOGY; PHOSPHATASES; RNA;

BIOLOGICAL PATHWAYS; MICRORNAS; NATURAL HYDROXYAPATITE; OSTEOINDUCTION; PROTEOMICS; TRANSCRIPTOMICS;

PROTEINS;

ALKALINE PHOSPHATASE; HYDROXYAPATITE; MESSENGER RNA; MICRORNA; MICRORNA 26A; MICRORNA 26B; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; STRESS ACTIVATED PROTEIN KINASE; UNCLASSIFIED DRUG; MITOGEN ACTIVATED PROTEIN KINASE; POWDER; PROTEOME; TETRAZOLIUM; THIAZOLE DERIVATIVE; THIAZOLYL BLUE; TRANSCRIPTOME;

ADIPOGENESIS; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; GENE EXPRESSION; INTRACELLULAR SIGNALING; MESENCHYMAL STEM CELL; MOUSE; MTT ASSAY; NONHUMAN; OSTEOBLAST; PROTEIN EXPRESSION; PROTEOMICS; RNA ANALYSIS; TRANSCRIPTOMICS; ANIMAL; BONE DEVELOPMENT; BONE MARROW CELL; CHEMISTRY; CULTURE TECHNIQUE; CYTOLOGY; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; INFRARED SPECTROSCOPY; MALE; MESENCHYMAL STROMA CELL; METABOLISM; PHYSIOLOGY; POWDER; SCANNING ELECTRON MICROSCOPY;

ALKALINE PHOSPHATASE; ANIMALS; BONE MARROW CELLS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; DURAPATITE; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; MALE; MESENCHYMAL STROMAL CELLS; MICE; MICRORNAS; MICROSCOPY, ELECTRON, SCANNING; OSTEOGENESIS; POWDERS; PROTEOME; PROTEOMICS; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TETRAZOLIUM SALTS; THIAZOLES; TRANSCRIPTOME;

EID: 84905050918     PISSN: 17486041     EISSN: 1748605X     Source Type: Journal    
DOI: 10.1088/1748-6041/9/4/045005     Document Type: Article

References (69)

1. Choi D and Kumta P N 2006 An alternative chemical route for the synthesis and thermal stability of chemically enriched hydroxyapatite J. Am. Ceram. Soc. 89 444-9
2. Huang Y C, Hsiao P C and Chai H J 2011 Hydroxyapatite extracted from fish scale: Effects on MG63 osteoblast-like cells Ceram. Int. 37 1825-31
3. Zhang X et al 2012 Osteoconductive effectiveness of bone graft derived from antler cancellous bone: an experimental study in the rabbit mandible defect model Int. J. Oral Maxillofac Surg. 41 1330-7
4. Lü X Y, Fan Y B, Gu D C and Cui W 2007 Preparation and characterization of natural hydroxyapatite from animal hard tissues Key Eng. Mater. 342-343 213-6 (Pubitemid 46558441)
5. Kurkcu M, Benlidayi M E, Cam B and Sertdemir Y 2012 Anorganic bovine-derived hydroxyapatite vs beta-tricalcium phosphate in sinus augmentation: a comparative histomorphometric study J. Oral Implantol 38 519-26
6. Rojbani H, Nyan M, Ohya K and Kasugai S 2011 Evaluation of the osteoconductivity of alpha-tricalcium phosphate, beta-tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect J. Biomed. Mater. Res. A 98 488-98
7. Heughebaert M, Legeros R Z, Gineste M, Guilhem A and Bonel G 1988 Physicochemical characterization of deposits associated with HA ceramics implanted in nonosseous sites J. Biomed. Mater. Res. B 22 257-68 (Pubitemid 19003136)
8. Yang Z J, Yuan H P, Tong W D, Zou P, Chen W Q and Zhang X D 1996 Osteogenesis in extraskeletally implanted porous calcium phosphate ceramics: variability among different kinds of animals Biomaterials 17 2131-7 (Pubitemid 26364766)
9. Ripamonti U 1996 Osteoinduction in porous hydroxyapatite implanted in heterotopic sites of different animal models Biomaterials 17 31-5 (Pubitemid 126755189)
10. Kim S S and Kim B S 2008 Comparison of osteogenic potential between apatite-coated poly(lactide-co-glycolide)/hydroxyapatite particulates and Bio-Oss (R) J. Dent. Mater. 27 368-75
11. Liu Y K et al 2009 In vitro effects of nanophase hydroxyapatite particles on proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells J. Biomed. Mater. Res. A 90A 1083-91
12. Hu Q H et al 2007 Effect of crystallinity of calcium phosphate nanoparticles on adhesion, proliferation, and differentiation of bone marrow mesenchymal stem cells J. mater. Chem.17 4690-8
13. Babister J C, Hails L A, Oreffo R O C, Davis S A and Mann S 2009 The effect of pre-coating human bone marrow stromal cells with hydroxyapatite/amino acid nanoconjugates on osteogenesis Biomaterials 30 3174-82
14. Luo E, Liu X, Wei S C, Cai X X and Hu J 2008 Osteoblast adhesion to clodronate-hydroxyapatite composite Appl. Surf. Sci. 255 308-11
15. Zhao H X et al 2011 The structural and biological properties of hydroxyapatite-modified titanate nanowire scaffolds Biomaterials 32 5837-46
16. Kim K, Dean D, Lu A Q, Mikos AG and Fisher J P 2011 Early osteogenic signal expression of rat bone marrow stromal cells is influenced by both hydroxyapatite nanoparticle content and initial cell seeding density in biodegradable nanocomposite scaffolds Acta Biomater. 7 1249-64
17. Lin LW, Chow L and Leng Y 2009 Study of hydroxyapatite osteoinductivity with an osteogenic differentiation of mesenchymal stem cells J. Biomed. Mater. Res. A 89A 326-35
18. Ripamonti U, Crooks J, Khoah L and Roden L 2009 The induction of bone formation by coral-derived calcium carbonate/hydroxyapatite constructs Biomaterials 30 1428-39
19. Xie J W, Baumann M J and McCabe L R 2004 Osteoblasts respond to hydroxyapatite surfaces with immediate changes in gene expression J. Biomed. Mater. Res. A 71A 108-17 (Pubitemid 39244401)
20. Song J H et al 2008 Signaling responses of osteoblast cells to hydroxyapatite: the activation of ERK and SOX9 J. Bone. Miner. Metab. 26 138-42
21. Xu J L, Khor K A, Sui J J, Zhang J H, Tan T L and Chen W N 2008 Comparative proteomics profile of osteoblasts cultured on dissimilar hydroxyapatite biomaterials: an iTRAQ-coupled 2-D LC-MS/MS analysis Proteomics 8 4249-58
22. Xu J L, Khor K A, Sui J J, Zhang J H and Chen W N 2009 Protein expression profiles in osteoblasts in response to differentially shaped hydroxyrapatite nanoparticles Biomaterials 30 5385-91
23. Lü X Y, Huang Y, Yu Y D and Yang Y M 2013 Application of genomics/proteomics technologies in the research of biocompatibility of biomaterials J. Inorg. Mater. 28 21-8 (in Chinese)
24. Tian Q et al 2004 Integrated genomic and proteomic analyses of gene expression in mammalian cells Mol. Cell. Proteomics 3 960-9 (Pubitemid 40361231)
25. Qu Y H, Huang Y and Lu X Y 2013 Proteomic analysis of molecular biocompatibility of gold nanoparticles to human dermal fibroblasts-fetal J. Biomed. Nanotechnol. 9 40-52
26. Bartel D P 2009 MicroRNAs: target recognition and regulatory functions Cell 136 215-33
27. Morozova N, Zinovyev A, Nonne N, Pritchard L L, Gorban A N and Harel-Bellan A 2012 Kinetic signatures of microRNA modes of action Rna 18 1635-55
28. Lian J B et al 2012 MicroRNA control of bone formation and homeostasis Nat. Rev. Endocrinol 8 212-27
29. Ivey K N and Srivastava D 2010 MicroRNAs as regulators of differentiation and cell fate decisions Cell Stem Cell 7 36-41
30. Takane K and Kanai A 2011 Vertebrate virus-encoded microRNAs and their sequence conservation Jpn. J. Infect. Dis. 64 357-66
31. Xiaoying Lü, Jiandan Wang, Bin Li, Zhiwei Zhang and Lifeng Zhao 2014 Gene expression profile study on osteoinductive effect of natural hydroxyapatite J. Biomed. Mater. Res. A, doi:10.1002/jbm.a.34951
32. Lü X Y, Huang Y, Zheng B Z and Wu A P 2007 Comparing adsorptive property of natural and synthesized hydroxyapatite to albumin, fibrinogen and IgG Key. Eng. Mater. 330-332 869-72 (Pubitemid 46120909)
33. Yang J et al 2012 Differentiation potential of human mesenchymal stem cells derived from adipose tissue and bone marrow to sinus node-like cells Mol. Med. Rep. 5 108-13
34. Lü X Y, Bao X, Huang Y, Qu Y H, Lu H Q and Lu Z H 2009 Mechanisms of cytotoxicity of nickel ions based on gene expression profiles Biomaterials 30 141-8
35. Arpornmaeklong P, Brown S E, Wang Z and Krebsbach P H 2009 Phenotypic characterization, osteoblastic differentiation, and bone regeneration capacity of human embryonic stem cell-derived mesenchymal stem cells Stem Cells Dev. 18 955-68
36. Dahlquist K D, Salomonis N, Vranizan K, Lawlor S C and Conklin B R 2002 GenMAPP, a new tool for viewing and analyzing microarray data on biological pathways Nat. Genet. 31 19-20
37. Megraw M, Sethupathy P, Corda B and Hatzigeorgiou A G 2007 miRGen: a database for the study of animal microRNA genomic organization and function Nucleic Acids Res. 35 D149-55 (Pubitemid 46056188)
38. Livak K J and Schmittgen T D 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-delta delta C) method Methods 25 402-8 (Pubitemid 34164012)
39. Solly K, Wang XB, Xu X, Strulovici B and Zheng W 2004 Application of real-time cell electronic sensing (RT-CES) technology to cell-based assays Assay Drug. Dev. Techn. 2 363-72 (Pubitemid 39276278)
40. Vandesompele J et al 2002 Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome. Biol. 3 1-11
41. Rupani A, Hidalgo-Bastida L A, Rutten F, Dent A, Turner I and Cartmell S 2012 Osteoblast activity on carbonated hydroxyapatite J. Biomed. Mater. Res. A 100A 1089-96
42. Figueiredo M, Fernando A, Martins G, Freitas J, Judas F and Figueiredo H 2010 Effect of the calcination temperature on the composition and microstructure of hydroxyapatite derived from human and animal bone Ceram. Int. 36 2383-93
43. Wexler S A, Donaldson C, Denning-Kendall P, Rice C, Bradley B and Hows J M 2003 Adult bone marrow is a rich source of human mesenchymal 'stem' cells but umbilical cord and mobilized adult blood are not Brit. J. Haematol. 121 368-74 (Pubitemid 36560606)
44. Arvidson K et al 2011 Bone regeneration and stem cells J. Cell Mol. Med. 15 718-46
45. McCabe L R, Kockx M, Lian J, Stein J and Stein G 1995 Selective expression of fos-and jun-related genes during osteoblast proliferation and differentiation Exp. Cell Res. 218 255-62
46. Bhandari D R, Seo K W, Jung J W, Kim H S, Yang S R and Kang K S 2011 The regulatory role of c-MYC on HDAC2 and PcG expression in human multipotent stem cells J. Cell. Mol. Med.15 1603-14
47. Franceschi R T, Ge C X, Xiao G Z, Roca H and Jiang D 2009 Transcriptional regulation of osteoblasts Cells Tissues Organs 189 144-52
48. Russell K C, Phinney D G, Lacey M R, Barrilleaux B L, Meyertholen K E and O'Connor K C 2010 In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment Stem Cells 28 788-98
49. Muraglia A, Cancedda R and Quarto R 2000 Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model J. Cell Sci. 113 1161-6 (Pubitemid 30229533)
50. Esposito F et al 2009 Interaction between HMGA1 and retinoblastoma protein is required for adipocyte differentiation J. Biol. Chem. 284 25993-6004
51. McBeath R, Pirone D M, Nelson C M, Bhadriraju K and Chen C S 2004 Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment Dev Cell 6 483-95 (Pubitemid 38456178)
52. Yarden Y and Sliwkowski M X 2001 Untangling the ErbB signalling network Nat. Rev. Mol. Cell Biol. 2127-37
53. Kusenda B, Mraz M, Mayer J and Pospisilova S 2006 MicroRNA biogenesis, functionality and cancer relevance Biomed. Pap. 150 205-15
54. Lim LP et al 2005 Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs Nature 433 769-73
55. Guo H L, Ingolia N T, Weissman J S and Bartel D P 2010 Mammalian microRNAs predominantly act to decrease target mRNA levels Nature 466 835-66
56. Guo Z et al 2013 MiR-23a regulates DNA damage repair and apoptosis in UVB-irradiated HaCaT cells J. Dermatol Sci. 69 68-76
57. Zhang X H et al 2012 Overexpression of miR-125b, a novel regulator of innate immunity, in eosinophilic chronic rhinosinusitis with nasal polyps Am. J. Resp. Crit. Care 185 140-51
58. Rajewsky N 2006 microRNA target predictions in animals Nat. Genet. 38 S8-S13
59. Li W, Yuan Y, Huang L, Qiao M and Zhang Y 2012 Metformin alters the expression profiles of microRNAs in human pancreatic cancer cells Diabetes Res. Clin. Pract. 96 187-95
60. Liu M et al 2011 miR-185 targets RhoA and Cdc42 expression and inhibits the proliferation potential of human colorectal cells Cancer Lett. 301151-60
61. Khurana J S 2009 Bone Pathology 2nd ed (New York: Humana Press)
62. Elabd C et al 2008 Oxytocin controls differentiation of human mesenchymal stem cells and reverses osteoporosis Stem Cells 26 2399-407
63. Akiyama H, Chaboissier M C, Martin J F, Schedl A and de Crombrugghe B 2002 The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6 Genes Dev 16 2813-28 (Pubitemid 35253149)
64. Zuo Y, Qiang L and Farmer S R 2006 Activation of CCAAT/enhancer-binding protein (C/EBP) alpha expression by C/EBP beta during adipogenesis requires a peroxisome proliferator-activated receptor-gamma-associated repression of HDAC1 at the C/ebp alpha gene promoter J. Biol. Chem. 2817960-7
65. Bellows C G, Aubin J E, Heersche J N M and Antosz M E 1986 Mineralized bone nodules formedin vitro from enzymatically released rat calvaria cell populations Calcified Tissue Int. 38 143-54 (Pubitemid 16160917)
66. Cowles E A, Brailey L L and Gronowicz G A 2000 Integrin-mediated signaling regulates AP-1 transcription factors and proliferation in osteoblasts J. Biomed. Mater. Res. 52 725-37
67. Arosarena O A, Del Carpio-Cano F E, Dela Cadena R A, Rico M C, Nwodim E and Safadi F F 2011 Comparison of bone morphogenetic protein-2 and osteoactivin for mesenchymal cell differentiation: effects of bolus and continuous administration J. Cell Physiol. 226 2943-52
68. Wozney J M and Rosen V 1998 Bone morphogenetic protein and bone morphogenetic protein gene family in bone formation and repair Clin. Orthop. Relat. Res. 346 26-37 (Pubitemid 28046864)
69. Noshi T et al 2001 Recombinant human bone morphogenetic protein-2 potentiates the in vivo osteogenic ability of marrow/hydroxyapatite composites Artif. Organs 25 201-8 (Pubitemid 32852968)