Integrating high-content analysis into a multiplexed screening approach to identify and characterize GPCR agonists

Journal of Biomolecular Screening

Volumn 19, Issue 7, 2014, Pages 1079-1089

  Zhu, Yingjie ^a   Watson, John ^a   Chen, Mengjie ^b   Shen, Ding Ren ^a   Yarde, Melissa ^a   Agler, Michele ^c   Burford, Neil ^a   Alt, Andrew ^a   Jayachandra, Sukhanya ^a   Cvijic, Mary Ellen ^a   Zhang, Litao ^a   Dyckman, Alaric ^a   Xie, Jenny ^a   O'Connell, Jonathan ^d   Banks, Martyn ^a   Weston, Andrea ^a  

^a BRISTOL MYERS SQUIBB   (United States)

^b YALE UNIVERSITY   (United States)

^c BOEHRINGER INGELHEIM PHARMACEUTICALS INC   (United States)

^d High Throughput Screening Forma Therapeutics   (United States)

Author keywords

cell based assays; G protein coupled receptors (GPCR); high content screening; image analysis

Indexed keywords

AEQUORIN; AGENTS INTERACTING WITH TRANSMITTER, HORMONE OR DRUG RECEPTORS; BETA ARRESTIN; G PROTEIN COUPLED RECEPTOR; G PROTEIN COUPLED RECEPTOR AGONIST; SPHINGOSINE 1 PHOSPHATE RECEPTOR; UNCLASSIFIED DRUG; CYCLIC AMP; GREEN FLUORESCENT PROTEIN; PROTEIN BINDING;

ANIMAL CELL; ARTICLE; CELL STRUCTURE; CELLULAR PARAMETERS; CHO CELL LINE; CONTROLLED STUDY; HIGH CONTENT IMAGING; IMAGE ANALYSIS; INTERNALIZATION; MOLECULAR IMAGING; NONHUMAN; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN TRANSLOCATION; RECEPTOR BINDING; RECEPTOR RECYCLING; RECEPTOR TRAFFICKING; U2OS CELL LINE; AGONISTS; ANIMAL; ANTAGONISTS AND INHIBITORS; BINDING SITE; BIOASSAY; CHEMISTRY; CRICETULUS; DRUG DEVELOPMENT; FLUORESCENCE MICROSCOPY; HAMSTER; HUMAN; IMAGE PROCESSING; METABOLISM; PROCEDURES; PROTEIN TRANSPORT; RAT; REPRODUCIBILITY; TUMOR CELL LINE;

ALLOSTERIC SITE; ANIMALS; BETA-ARRESTINS; BIOLOGICAL ASSAY; CELL LINE, TUMOR; CHO CELLS; CRICETINAE; CRICETULUS; CYCLIC AMP; DRUG DISCOVERY; GREEN FLUORESCENT PROTEINS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE; PROTEIN BINDING; PROTEIN TRANSPORT; RATS; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, LYSOSPHINGOLIPID; REPRODUCIBILITY OF RESULTS;

EID: 84904697705     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057114533146     Document Type: Article

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