GPCR-induced dissociation of G-protein subunits in early stage signal transduction

Molecular Membrane Biology

Volumn 22, Issue 6, 2005, Pages 507-517

  Leifert, Wayne R ^a   Aloia, Amanda L ^a,b   Bucco, Olgatina ^a,c   Mcmurchie, Edward J ^a  

^a CSIRO   (Australia)

^b School of Biological Sciences

^c FLINDERS UNIVERSITY   (Australia)

Author keywords

Dissociation; G protein coupled receptor; G proteins; Receptor; Signalling

Indexed keywords

ALPHA 2 ADRENERGIC RECEPTOR; BRIMONIDINE; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); HISTIDINE; IMIDAZOLE; NICKEL; PROTEIN SUBUNIT; SULFUR 35;

ANIMAL CELL; ARTICLE; CELL FREE SYSTEM; COMPLEX FORMATION; CONTROLLED STUDY; DRUG POTENCY; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

ANIMALS; CELL MEMBRANE; GTP-BINDING PROTEINS; HUMANS; MEMBRANES, ARTIFICIAL; PROTEIN BINDING; RECEPTORS, ADRENERGIC, ALPHA-2; SIGNAL TRANSDUCTION;

ANIMALIA;

EID: 29744437881     PISSN: 09687688     EISSN: 14645203     Source Type: Journal    
DOI: 10.1080/09687860500370604     Document Type: Article

References (31)

1. Wise A, Gearing K, Rees S. Target validation of G-protein coupled receptors. Drug Discov Today 2002;7:235-246.
2. Vassilatis DK, Hohmann JG, Zeng H, Li F, Ranchalis JE, Mortrud MT, Brown A, Rodriguez SS, Weller JR, Wright AC, Bergmann JE, Gaitanaris GA. The G protein-coupled receptor repertoires of human and mouse. Proc Natl Acad Sci USA 2003;100:4903-4908.
3. Gales C, Rebois RV, Hogue M, Trieu P, Breit A, Hebert TE, Bouvier M. Real-time monitoring of receptor and G-protein interactions in living cells. Nat Methods 2005;2:177-184.
4. Neer EJ. Heterotrimeric G proteins: organizers of transmembrane signals. Cell 1995;80:249-257.
5. Hepler JR, Gilman AG. G proteins. Trends Biochem Sci 1992;17:383-387.
6. 2. Cell 1995;83:1047-1058.
7. 1 induced by GTP hydrolysis. Science 1995;270:954-960.
8. Lambright DG, Sondek J, Bohm A, Skiba NP, Hamm HE, Sigler PB. The 2.0 A crystal structure of a heterotrimeric G-protein. Nature 1996;379:311-319.
9. Watson N, Linder ME, Druey KM, Kehrl JH, Blumer KJ. RGS family members: GTPase-activating proteins for heterotrimeric G-protein alpha-subunits. Nature 1996;383:172-175.
10. Druey KM, Blumer KJ, Kang VH, Kehrl JH. Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family. Nature 1996;379:742-746.
11. + channels. Proc Natl Acad Sci USA 1997;94:10461-10466.
12. Sherwood L. The plasma membrane and membrane potential. In: Sherwood L, editor. Human physiology - from cells to systems. 3rd ed. Belmont, CA: Wadsworth; 1997. pp 46-80.
13. Goding JW. The plasma-membrane receptors and GTP-binding proteins. In: Barritt GJ, editor. Communication within animal cells. New York: Oxford University Press; 1992. pp 53-89.
14. i-specific region. J Biol Chem 2005;280:24584-24590.
15. Bieri C, Ernst OP, Heyse S, Hofmann KP, Vogel H. Micropatterned immobilization of a G protein-coupled receptor and direct detection of G-protein activation. Nat Biotechnol 1999;17:1105-1108.
16. Simons PC, Shi M, Foutz T, Cimino DF, Lewis J, Buranda T, Lim WK, Neubig RR, McIntire WE, Garrison J, Prossnitz E, Sklar LA. Ligand-receptor-G-protein molecular assemblies on beads for mechanistic studies and screening by flow cytometry. Mol Pharmacol 2003;64:1227-1238.
17. 35S] GTPγS binding assays. Trends Pharmacol Sci 2003;24:87-90.
18. 35S]GTPγS binding assay: Approaches and applications in pharmacology. Life Sci 2003;74:489-508.
19. Massotte D. G protein-coupled receptor overexpression with the baculovirus-insect cell system: A tool for structural and functional studies. Biochim Biophys Acta 2003;1610:77-89.
20. Lim WK, Neubig RR. Selective inactivation of guanine-nucleotide-binding regulatory protein (G-protein) α and βγ; subunits by urea. Biochem J 2001;354:337-344.
21. Z. J Biol Chem 1995;270:1734-1741.
22. Wei Q, Zhou DH, Shen QX, Chen J, Chen LW, Wang TL, Pei G, Chi ZQ. Human mu-opioid receptor overexpressed in Sf9 insect cells functionally coupled to endogenous Gi/o proteins. Cell Res 2000;10:93-102.
23. Knight PJ, Grigliatti TA. Diversity of G proteins in Lepidopteran cell lines: Partial sequences of six G protein alpha subunits. Arch Insect Biochem Physiol 2004;57:142-150.
24. 3H]-MK912 is alpha-2C selective among human alpha-2A, alpha-2B and alpha-2C adrenoceptors. J Pharmacol Exp Ther 1994;271:1558-1565.
25. Simons PC, Biggs SM, Waller A, Foutz T, Cimino DF, Guo Q, Neubig RR, Tang WJ, Prossnitz ER, Sklar LA. Real-time analysis of ternary complex on particles: Direct evidence for partial agonism at the agonist-receptor-G protein complex assembly step of signal transduction. J Biol Chem 2004;279:13514-13521.
26. Sarvazyan NA, Lim WK, Neubig RR. Fluorescence analysis of receptor-G protein interactions in cell membranes. Biochemistry 2002;41:12858-12867.
27. Waller A, Simons PC, Biggs SM, Edwards BS, Prossnitz ER, Sklar LA. Techniques: GPCR assembly, pharmacology and screening by flow cytometry. Trends Pharmacol Sci 2004;25:663-669.
28. Waller A, Simons P, Prossnitz ER, Edwards BS, Sklar LA. High throughput screening of G-protein coupled receptors via flow cytometry. Comb Chem High Throughput Screen 2003;6:389-397.
29. Bennett TA, Key TA, Gurevich W, Neubig RR, Prossnitz ER, Sklar LA. Real-time analysis of G protein-coupled receptor reconstitution in a solubilized system. J Biol Chem 2001;276:22453-22460.
30. Sklar LA, Vilven J, Lynam E, Neldon D, Bennett TA, Prossnitz E. Solubilization and display of G protein-coupled receptors on beads for real-time fluorescence and flow cytometric analysis. BioTechniques 2000;28:976-975.
31. Bunemann M, Frank M, Lohse MJ. Gi protein activation in intact cells involves subunit rearrangement rather than dissociation. Proc Natl Acad Sci USA 2003;100:16077-16082.