Enhanced catalytic efficiency in quercetin-4′-glucoside hydrolysis of thermotoga maritima β-glucosidase a by site-directed mutagenesis

Journal of Agricultural and Food Chemistry

Volumn 62, Issue 28, 2014, Pages 6763-6770

  Sun, Huihui ^a   Xue, Yemin ^a   Lin, Yufei ^a  

^a NANJING NORMAL UNIVERSITY   (China)

Author keywords

catlytic efficiency; protein engineering; quercetin 4 glucoside hydrolysis; Thermotoga maritima; flucosidase A

Indexed keywords

AMINO ACIDS; CARBOHYDRATES; EFFICIENCY; ENZYMATIC HYDROLYSIS; ENZYME ACTIVITY; PHENOLS; FLAVONOIDS; GLYCOSIDES; HYDROLYSIS; MUTAGENESIS; SUGARS;

AMINO ACID RESIDUES; CATALYTIC EFFICIENCIES; GLYCOSIDE HYDROLASE FAMILY 1; PROTEIN ENGINEERING; QUERCETIN GLUCOSIDES; SITE DIRECTED MUTAGENESIS; SUBSTRATE SPECIFICITY; THERMOTOGA MARITIMA; OPTIMAL TEMPERATURE;

FLAVONOIDS; ENZYMATIC HYDROLYSIS;

BETA GLUCOSIDASE; ISOFLAVONE DERIVATIVE; QUERCETIN; SPIRAEOSIDE;

AMINO ACID SEQUENCE; ANALOGS AND DERIVATIVES; CATALYSIS; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; HYDROLYSIS; KINETICS; METABOLISM; MOLECULAR GENETICS; PH; PROCEDURES; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; THERMOTOGA MARITIMA;

AMINO ACID SEQUENCE; BETA-GLUCOSIDASE; CATALYSIS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; ISOFLAVONES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; QUERCETIN; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; THERMOTOGA MARITIMA;

THERMOANAEROBACTER ETHANOLICUS; THERMOTOGA MARITIMA;

EID: 84904409336     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf501932v     Document Type: Article

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