High-throughput absolute quantification of proteins using an improved two-dimensional reversed-phase separation and quantification concatemer (QconCAT) approach

Analytical and Bioanalytical Chemistry

Volumn 406, Issue 17, 2014, Pages 4183-4193

  Wei, Junying ^a   Ding, Chen ^b   Zhang, Jiao ^b   Mi, Wei ^c   Zhao, Yan ^b   Liu, Mingwei ^b   Fu, Tianyi ^b   Zhang, Yangjun ^b   Ying, Wantao ^b   Cai, Yun ^b   Qin, Jun ^b   Qian, Xiaohong ^b  

^a INSTITUTE OF CHINESE MATERIA MEDICA   (China)

^b BEIJING INSTITUTE OF RADIATION MEDICINE   (China)

^c NATIONAL INSTITUTE OF METROLOGY   (China)

Author keywords

Absolute quantification; Proteome; Quantification concatemer (QconCAT); Two dimensional reversed phase separation

Indexed keywords

CALIBRATION; CHEMICAL ANALYSIS; ELECTROPHORESIS; ISOTOPES; MASS SPECTROMETRY; PHASE SEPARATION; STANDARDS; TISSUE; TISSUE ENGINEERING; TWO DIMENSIONAL;

ABSOLUTE QUANTIFICATION; GLUTATHIONE-S-TRANSFERASE; HIGH-THROUGHPUT APPROACHES; INTERNAL STANDARDS; MEASUREMENT TIME; QUANTIFICATION CONCATEMER (QCONCAT); REVERSED-PHASE SEPARATION; SEPARATION METHODS;

PROTEINS;

GLUTATHIONE TRANSFERASE; PEPTIDE; PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; DEVICES; EVALUATION STUDY; HIGH THROUGHPUT SCREENING; LIVER; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; MOUSE; PROCEDURES; PROTEOMICS; REVERSED PHASE LIQUID CHROMATOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, REVERSE-PHASE; GLUTATHIONE TRANSFERASE; HIGH-THROUGHPUT SCREENING ASSAYS; LIVER; MASS SPECTROMETRY; MICE; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEINS; PROTEOMICS;

EID: 84903751906     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-014-7784-x     Document Type: Article

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