Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 97, Issue 7, 2013, Pages 2939-2949

  Chen, Lin ^a,b   Chen, Zhen ^b   Zheng, Ping ^b,c,d   Sun, Jibin ^b,c,d   Zeng, An Ping ^b  

^a TIANJIN UNIVERSITY   (China)

^b HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

^c INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^d Chinese Academy of Sciences   (China)

Author keywords

Feedback inhibition; L isoleucine; L valine; Threonine deaminase

Indexed keywords

COMPUTATIONAL APPROACH; CONCENTRATION-DEPENDENT; FEEDBACK INHIBITION; L-ISOLEUCINE; L-VALINE; POTENTIAL BINDING SITES; SITE DIRECTED MUTAGENESIS; THREONINE DEAMINASE;

BIOSYNTHESIS; ENZYME INHIBITION; ESCHERICHIA COLI; REENGINEERING;

AMINO ACIDS;

ISOLEUCINE; MONOMER; THREONINE DEHYDRATASE; VALINE;

BIOENGINEERING; CHEMICAL BINDING; COLIFORM BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; INHIBITION;

ALLOSTERISM; ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; BINDING SITE; CHEMICAL ENGINEERING; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME KINETICS; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE OVEREXPRESSION; NONHUMAN; SITE DIRECTED MUTAGENESIS; WILD TYPE;

ALLOSTERIC REGULATION; BINDING SITES; COMPUTATIONAL BIOLOGY; ESCHERICHIA COLI; ISOLEUCINE; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; THREONINE DEHYDRATASE; VALINE;

ESCHERICHIA COLI;

EID: 84876686644     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4176-z     Document Type: Article

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