Structure-guided engineering of the coenzyme specificity of Pseudomonas fluorescens mannitol 2-dehydrogenase to enable efficient utilization of NAD(H) and NADP(H)

FEBS Letters

Volumn 582, Issue 2, 2008, Pages 233-237

  Bubner, Patricia ^a   Klimacek, Mario ^a   Nidetzky, Bernd ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Alcohol dehydrogenase; Coenzyme specificity; Hydrogen bond geometry; Polyol specific long chain dehydrogenases reductases; Protein engineering; Rossmann fold

Indexed keywords

ALANINE; GLUTAMIC ACID; GLYCINE; HYDROXYL GROUP; LYSINE; MANNITOL DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; COENZYME; ELECTRICITY; ENZYME SPECIFICITY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS;

BASE SEQUENCE; COENZYMES; DNA PRIMERS; KINETICS; MANNITOL DEHYDROGENASES; MUTAGENESIS, SITE-DIRECTED; NAD; NADP; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PSEUDOMONAS FLUORESCENS; SUBSTRATE SPECIFICITY;

PSEUDOMONAS FLUORESCENS;

EID: 38049044961     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.12.008     Document Type: Article

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