The bias associated with amplicon sequencing does not affect the quantitative assessment of bacterial community dynamics

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Ibarbalz, Federico M ^a   Pérez, María Victoria ^a,b   Figuerola, Eva L M ^a   Erijman, Leonardo ^a,c  

^a INSTITUTO DE INVESTIGACIONES EN INGENIERÍA GENÉTICA Y BIOLOGÍA MOLECULAR   (Argentina)

^b Agua Y Saneamientos Argentinos SA (AySA)   (Argentina)

^c UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S; SEWAGE;

ACTIVATED SLUDGE; AMPLICON; ARTICLE; COMMUNITY DYNAMICS; FLUORESCENCE IN SITU HYBRIDIZATION; GENE SEQUENCE; GENETIC VARIABILITY; HIGH THROUGHPUT SEQUENCING; MICROBIAL COMMUNITY; NONHUMAN; POPULATION ABUNDANCE; QUANTITATIVE ANALYSIS; TAXON; TEMPERATURE; THIOTHRIX; ANALYSIS; BACTERIAL GENE; BACTERIUM; BIODIVERSITY; CLASSIFICATION; DNA SEQUENCE; DUPLICATE GENE; GENE AMPLIFICATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; MICROBIAL CONSORTIUM; MICROBIOLOGY; PROCEDURES; RNA GENE; SEWAGE; STATISTICAL BIAS; STATISTICS AND NUMERICAL DATA;

BACTERIA; BIAS (EPIDEMIOLOGY); BIODIVERSITY; GENE AMPLIFICATION; GENES, BACTERIAL; GENES, DUPLICATE; GENES, RRNA; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MICROBIAL CONSORTIA; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SEWAGE;

EID: 84903379679     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0099722     Document Type: Article

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