Comparison of species richness estimates obtained using nearly complete fragments and simulated pyrosequencing-generated fragments in 16S rRNA gene-based environmental surveys

Applied and Environmental Microbiology

Volumn 75, Issue 16, 2009, Pages 5227-5236

  Youssef, Noha ^a   Sheik, Cody S ^b   Krumholz, Lee R ^b   Najar, Fares Z ^b   Roe, Bruce A ^b   Elshahed, Mostafa S ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA; 16S RRNA GENE; ALIGNMENT METHODS; BACTERIAL COMMUNITY; BASE PAIRS; CLONE LIBRARY; DATA SETS; EAST PACIFIC RISE; ENVIRONMENTAL SURVEYS; NON-PARAMETRIC; OPERATIONAL TAXONOMIC UNITS; PAIRWISE DISTANCES; PYROSEQUENCING; SCATTER PLOTS; SPECIES RICHNESS; TALLGRASS PRAIRIE; ZEBRAFISH;

ALIGNMENT; CLONING; REGRESSION ANALYSIS; SOILS; SURVEYS;

FORESTRY;

DNA FRAGMENT; RNA 16S;

BACTERIUM; COMPARATIVE STUDY; DATA SET; GENE EXPRESSION; MICROBIAL COMMUNITY; PRAIRIE; REGRESSION ANALYSIS; RNA; SPECIES RICHNESS; TAXONOMY;

ARTICLE; BASALT; CHEMICAL COMPOSITION; CLONE; CONTROLLED STUDY; ECOSYSTEM; ENVIRONMENTAL IMPACT; GENE LIBRARY; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERATIONAL TAXONOMIC UNITS; PRAIRIE; PYROSEQUENCING; SEQUENCE ANALYSIS; SOIL; SPECIES RICHNESS; TAXONOMIC RANK; UNINDEXED SEQUENCE; ZEBRA FISH;

BACTERIA; ECOSYSTEM; GENES, RRNA; MOLECULAR SEQUENCE DATA; OKLAHOMA; POACEAE; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SOIL MICROBIOLOGY; SPECIES SPECIFICITY;

BACTERIA (MICROORGANISMS); DANIO RERIO; OTUS;

EID: 68549130740     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00592-09     Document Type: Article

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