Ultrafast fluorescence imaging in vivo with conjugated polymer fluorophores in the second near-infrared window

Nature Communications

Volumn 5, Issue , 2014, Pages

  Hong, Guosong ^a   Zou, Yingping ^b   Antaris, Alexander L ^a   Diao, Shuo ^a   Wu, Di ^a   Cheng, Kai ^c   Zhang, Xiaodong ^a   Chen, Changxin ^a   Liu, Bo ^b   He, Yuehui ^b   Wu, Justin Z ^a   Yuan, Jun ^b   Zhang, Bo ^a   Tao, Zhimin ^a   Fukunaga, Chihiro ^a   Dai, Hongjie ^a  

^a STANFORD UNIVERSITY   (United States)

^b CENTRAL SOUTH UNIVERSITY   (China)

^c STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COPOLYMER; EPIDERMAL GROWTH FACTOR RECEPTOR; FLUOROPHORE; MACROGOL; NANOPARTICLE; PHOSPHOLIPID; POLYMER; UNCLASSIFIED DRUG; MACROGOL DERIVATIVE;

FLUORESCENCE; NANOTECHNOLOGY; POLYMER; RODENT; SPATIAL RESOLUTION; WAVELENGTH;

ABSORPTION SPECTROSCOPY; ANIMAL EXPERIMENT; ARTERY BLOOD FLOW; ARTICLE; ATOMIC FORCE MICROSCOPY; BIOCOMPATIBILITY; BLEACHING; BLOOD FLOW; BLOOD FLOW VELOCITY; CANCER CELL; CARDIAC GATED IMAGING; CELL MEMBRANE; CELL SURFACE; CONJUGATE; CONTROLLED STUDY; CROSS LINKING; DOPPLER FLOWMETRY; ELECTROCARDIOGRAM; FEMALE; FEMORAL ARTERY; FLUORESCENCE; FLUORESCENCE IMAGING; GEL PERMEATION CHROMATOGRAPHY; HEART CYCLE; HEART RATE; HEART VENTRICLE EJECTION; IN VIVO STUDY; LIGHT; MOLECULAR IMAGING; MOLECULAR WEIGHT; MOUSE; NEAR INFRARED WINDOW; NONHUMAN; OPTICAL COHERENCE TOMOGRAPHY; OSCILLATION; POLYMERIZATION; PROTON NUCLEAR MAGNETIC RESONANCE; QUANTUM YIELD; SYNTHESIS; TIME; ULTRAFAST FLUORESCENCE IMAGING; ULTRASOUND; WAVEFORM; ANIMAL; ARTERY; CHEMICAL STRUCTURE; CHEMISTRY; DIAGNOSTIC USE; PHYSIOLOGY; PROCEDURES;

ANIMALS; ARTERIES; MICE; MICROSCOPY, ATOMIC FORCE; MOLECULAR IMAGING; MOLECULAR STRUCTURE; NANOPARTICLES; OPTICAL IMAGING; PHOSPHOLIPIDS; POLYETHYLENE GLYCOLS; POLYMERS; REGIONAL BLOOD FLOW;

EID: 84903155801     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms5206     Document Type: Article

References (67)

1. Welsher, K. et al. A route to brightly fluorescent carbon nanotubes for nearinfrared imaging in mice. Nat. Nanotechnol. 4, 773-780 (2009).
2. Hong, G. S. et al. Three-dimensional imaging of single nanotube molecule endocytosis on plasmonic substrates. Nat. Commun. 3, 700 (2012).
3. Hong, G. S. et al. Multifunctional in vivo vascular imaging using near-infrared II fluorescence. Nat. Med. 18, 1841-1846 (2012).
4. Won, N. et al. Imaging depths of near-infrared quantum dots in first and second optical windows. Mol. Imaging 11, 338-352 (2012).
5. Yi, H. J. et al. M13 phage-functionalized single-walled carbon nanotubes as nanoprobes for second near-infrared window fluorescence imaging of targeted tumors. Nano Lett. 12, 1176-1183 (2012).
6. Hong, G. S. et al. In vivo fluorescence imaging with Ag2S quantum dots in the second near-infrared region. Angew. Chem. Int. Ed. 51, 9818-9821 (2012).
7. Dong, B. H. et al. Facile synthesis of highly photoluminescent Ag2Se quantum dots as a new fluorescent probe in the second near-infrared window for in vivo imaging. Chem. Mater. 25, 2503-2509 (2013).
8. Naczynski, D. J. et al. Rare-earth-doped biological composites as in vivo shortwave infrared reporters. Nat. Commun. 4, 2199 (2013).
9. Welsher, K., Sherlock, S. P. & Dai, H. J. Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared window. Proc. Natl Acad. Sci. USA 108, 8943-8948 (2011).
10. Robinson, J. T. et al. High performance in vivo near-IR (4 1 mm) imaging and photothermal cancer therapy with carbon nanotubes. Nano Res. 3, 779-793 (2010).
11. Robinson, J. T. et al. In vivo fluorescence imaging in the second near-infrared window with long circulating carbon nanotubes capable of ultrahigh tumor uptake. J. Am. Chem. Soc. 134, 10664-10669 (2012).
12. Diao, S. et al. Chirality enriched (12,1) and (11,3) single-walled carbon nanotubes for biological imaging. J. Am. Chem. Soc. 134, 16971-16974 (2012).
13. Liu, Z. et al. Multiplexed five-color molecular imaging of cancer cells and tumor tissues with carbon nanotube raman tags in the near-infrared. Nano Res. 3, 222-233 (2010).
14. Liu, Z., Tabakman, S., Welsher, K. & Dai, H. J. Carbon nanotubes in biology and medicine: in vitro and in vivo detection, imaging and drug delivery. Nano Res. 2, 85-120 (2009).
15. Zhang, Y. et al. Ag2S quantum dot: a bright and biocompatible fluorescent nanoprobe in the second near-infrared window. ACS Nano 6, 3695-3702 (2012).
16. Zhang, Y. et al. Biodistribution, pharmacokinetics and toxicology of Ag2S nearinfrared quantum dots in mice. Biomaterials 34, 3639-3646 (2013).
17. Zhu, C. N. et al. Ag2Se quantum dots with tunable emission in the second nearinfrared window. ACS Appl. Mater. Interfaces 5, 1186-1189 (2013).
18. Tao, Z. M. et al. Biological imaging using nanoparticles of small organic molecules with fluorescence emission at wavelengths longer than 1,000 nm. Angew. Chem. Int. Ed. 52, 13002-13006 (2013).
19. Liu, Z. et al. Circulation and long-term fate of functionalized, biocompatible single-walled carbon nanotubes in mice probed by Raman spectroscopy. Proc. Natl Acad. Sci. USA 105, 1410-1415 (2008). (Pubitemid 351346531)
20. Schipper, M. L. et al. A pilot toxicology study of single-walled carbon nanotubes in a small sample of mice. Nat. Nanotechnol. 3, 216-221 (2008). (Pubitemid 351506336)
21. O'Connell, M. J. et al. Band gap fluorescence from individual single-walled carbon nanotubes. Science 297, 593-596 (2002). (Pubitemid 34815341)
22. Zhang, Q. T. & Tour, J. M. Low optical bandgap polythiophenes by an alternating donor/acceptor repeat unit strategy. J. Am. Chem. Soc. 119, 5065-5066 (1997). (Pubitemid 27272506)
23. Greenham, N. C., Moratti, S. C., Bradley, D. D. C., Friend, R. H. & Holmes, A. B. Efficient light-emitting-diodes based on polymers with high electron-affinities. Nature 365, 628-630 (1993).
24. Zhang, Z. G. et al. Alkyl chain engineering on a dithieno[3,2-b:2',3'-d] silole-altdithienylthiazolo[ 5,4-d]thiazole copolymer toward high performance bulk heterojunction solar cells. Chem. Commun. 47, 9474-9476 (2011).
25. Cui, C. H. et al. A D-A copolymer of dithienosilole and a new acceptor unit of naphtho[2,3-c]thiophene-4,9-dione for efficient polymer solar cells. Chem. Commun. 47, 11345-11347 (2011).
26. Liu, B. et al. A benzo[1,2-b: 4,5-b ']difuran-and thieno-[3,4-b] thiophene-based low bandgap copolymer for photovoltaic applications. Polym. Chem. 4, 470-476 (2013).
27. Yamamoto, T. et al. pi-conjugated donor-acceptor copolymers constituted of pi-excessive and pi-deficient arylene units. Optical and electrochemical properties in relation to CT structure of the polymer. J. Am. Chem. Soc. 118, 10389-10399 (1996). (Pubitemid 26381220)
28. Huo, L. J. et al. Conjugated and nonconjugated substitution effect on photovoltaic properties of benzodifuran-based photovoltaic polymers. Macromolecules 45, 6923-6929 (2012).
29. Huo, L. J. et al. Bandgap and molecular level control of the low-bandgap polymers based on 3,6-Dithiophen-2-yl-2,5-dihydropyrrolo[3,4-c]pyrrole-1,4-dione toward highly efficient polymer solar cells. Macromolecules 42, 6564-6571 (2009).
30. Cornil, J. et al. Electronic and optical properties of polyfluorene and fluorenebased copolymers: a quantum-chemical characterization. J. Chem. Phys. 118, 6615-6623 (2003).
31. Murphy, J. E. et al. PbTe colloidal nanocrystals: synthesis, characterization, and multiple exciton generation. J. Am. Chem. Soc. 128, 3241-3247 (2006).
32. Williams, A. T. R., Winfield, S. A. & Miller, J. N. Relative fluorescence quantum yields using a computer-controlled luminescence spectrometer. Analyst 108, 1067-1071 (1983).
33. Liu, Z., Tabakman, S. M., Chen, Z. & Dai, H. J. Preparation of carbon nanotube bioconjugates for biomedical applications. Nat. Protoc. 4, 1372-1382 (2009).
34. Hong, G. S. et al. Near-infrared-fluorescence-enhanced molecular imaging of live cells on gold substrates. Angew. Chem. Int. Ed. 50, 4644-4648 (2011).
35. Riccabona, M., Nelson, T. R., Pretorius, D. H. & Davidson, T. E. Distance and volume measurement using 3-dimensional ultrasonography. J. Ultrasound Med. 14, 881-886 (1995).
36. Tomlins, P. H. &Wang, R. K. Theory, developments and applications of optical coherence tomography. J. Phys. D Appl. Phys. 38, 2519-2535 (2005). (Pubitemid 41067740)
37. Wladimiroff, J. W., Tonge, H. M. & Stewart, P. A. Doppler ultrasound assessment of cerebral blood-flow in the human-fetus. Br. J. Obstet. Gynaecol. 93, 471-475 (1986). (Pubitemid 16070042)
38. Lee, S. et al. Real-time in vivo imaging of the beating mouse heart at microscopic resolution. Nat. Commun. 3, 1054 (2012).
39. Li, G. et al. High-efficiency solution processable polymer photovoltaic cells by self-organization of polymer blends. Nat. Mater. 4, 864-868 (2005).
40. Burroughes, J. H. et al. Light-emitting-diodes based on conjugated polymers. Nature 347, 539-541 (1990).
41. Kawamura, Y., Yanagida, S. & Forrest, S. R. Energy transfer in polymer electrophosphorescent light emitting devices with single and multiple doped luminescent layers. J. Appl. Phys. 92, 87-93 (2002).
42. Facchetti, A. pi-conjugated polymers for organic electronics and photovoltaic cell applications. Chem. Mater. 23, 733-758 (2011).
43. Ong, B. S., Wu, Y. L., Liu, P. & Gardner, S. High-performance semiconducting polythiophenes for organic thin-film transistors. J. Am. Chem. Soc. 126, 3378-3379 (2004). (Pubitemid 38372119)
44. Ghoroghchian, P. P. et al. Near-infrared-emissive polymersomes: Selfassembled soft matter for in vivo optical imaging. Proc. Natl Acad. Sci. USA 102, 2922-2927 (2005). (Pubitemid 40299892)
45. Jin, Y. H., Ye, F. M., Zeigler, M., Wu, C. F. & Chiu, D. T. Near-infrared fluorescent dye-doped semiconducting polymer dots. ACS Nano 5, 1468-1475 (2011).
46. Pu, K. Y., Li, K. & Liu, B. A molecular brush approach to enhance quantum yield and suppress nonspecific interactions of conjugated polyelectrolyte for targeted far-red/near-infrared fluorescence cell imaging. Adv. Funct. Mater. 20, 2770-2777 (2010).
47. Pu, K. Y. & Liu, B. Fluorescent conjugated polyelectrolytes for bioimaging. Adv. Funct. Mater. 21, 3408-3423 (2011).
48. Wang, Y. L. et al. Tuning the electronic structures and optical properties of fluorene-based donor-acceptor copolymers by changing the acceptors: a theoretical study. Theor. Chem. Acc. 129, 257-270 (2011).
49. Wiesmann, F. et al. High-resolution MRI with cardiac and respiratory gating allows for accurate in vivo atherosclerotic plaque visualization in the murine aortic arch. Magnet. Reson. Med. 50, 69-74 (2003). (Pubitemid 36841861)
50. Gioux, S., Ashitate, Y., Hutteman, M. & Frangioni, J. V. Motion-gated acquisition for in vivo optical imaging. J. Biomed. Opt. 14, 064038 (2009).
51. Li, P., Yin, X., Shi, L., Rugonyi, S. & Wang, R. K. K. In vivo functional imaging of blood flow and wall strain rate in outflow tract of embryonic chick heart using ultrafast spectral domain optical coherence tomography. J. Biomed. Opt. 17, 96006-1 (2012).
52. An, L., Guan, G. Y. & Wang, R. K. K. High-speed 1310 nm-band spectral domain optical coherence tomography at 184,000 lines per second. J. Biomed. Opt. 16, 060506 (2011).
53. Gill, R. W. Measurement of blood-flow by ultrasound-accuracy and sources of error. Ultrasound Med. Biol. 11, 625-641 (1985). (Pubitemid 15000123)
54. Xuan, J. W. et al. Functional neoangiogenesis imaging of genetically engineered mouse prostate cancer using three-dimensional power Doppler ultrasound. Cancer Res. 67, 2830-2839 (2007). (Pubitemid 46548973)
55. Adler, D. C., Ko, T. H. & Fujimoto, J. G. Speckle reduction in optical coherence tomography images by use of a spatially adaptive wavelet filter. Opt. Lett. 29, 2878-2880 (2004). (Pubitemid 40011254)
56. Abd-Elmoniem, K. Z., Youssef, A. B. M. & Kadah, Y. M. Real-time speckle reduction and coherence enhancement in ultrasound imaging via nonlinear anisotropic diffusion. IEEE T. Bio. Med. Eng. 49, 997-1014 (2002). (Pubitemid 34925468)
57. Hong, G. et al. Near-infrared II fluorescence for imaging hindlimb vessel regeneration with dynamic tissue perfusion measurement. Circ. Cardiovasc. Imaging 7, 517-525 (2014).
58. Lin, W. C. et al. Red blood cell velocity measurement in rodent tumor model: an in vivo microscopic study. J. Med. Biol. Eng. 32, 97-102 (2012).
59. An, L., Qin, J. & Wang, R. K. Ultrahigh sensitive optical microangiography for in vivo imaging of microcirculations within human skin tissue beds. Opt. Express 18, 8220-8228 (2010).
60. Tian, Z. Y., Yu, J. B., Wu, C. F., Szymanski, C. & McNeill, J. Amplified energy transfer in conjugated polymer nanoparticle tags and sensors. Nanoscale 2, 1999-2011 (2010).
61. Schrier, R. W. & Abraham, W. T. Mechanisms of disease-hormones and hemodynamics in heart failure. N. Engl. J. Med. 341, 577-585 (1999). (Pubitemid 29384167)
62. Liang, Y. Y. et al. Highly efficient solar cell polymers developed via fine-tuning of structural and electronic properties. J. Am. Chem. Soc. 131, 7792-7799 (2009).
63. Huo, L. J. et al. Synthesis of a 4,8-dialkoxy-benzo[1,2-b:4,5-b']difuran unit and its application in photovoltaic polymer. Chem. Commun. 48, 3318-3320 (2012).
64. Chen, X. W. et al. A new benzo[1,2-b:4,5-b']difuran-based copolymer for efficient polymer solar cells. J. Mater. Chem. 22, 17724-17731 (2012).
65. Wurth, C., Grabolle, M., Pauli, J., Spieles, M. & Resch-Genger, U. Relative and absolute determination of fluorescence quantum yields of transparent samples. Nat. Protoc. 8, 1535-1550 (2013).
66. Keller, D. Reconstruction of STM and AFM images distorted by finite-size tips. Surf. Sci. 253, 353-364 (1991). (Pubitemid 21721712)
67. Matthes, R. et al. Revision of guidelines on limits of exposure to laser radiation of wavelengths between 400nm and 1.4 mu m. Health. Phys. 79, 431-440 (2000).