Rapid detection of aflatoxin producing fungi in food by real-time quantitative loop-mediated isothermal amplification

Food Microbiology

Volumn 44, Issue , 2014, Pages

  Luo, Jie ^a   Vogel, Rudi F ^a   Niessen, Ludwig ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Aflatoxin; Aspergillus; Food; Real time LAMP; Spore

Indexed keywords

AFLATOXIN; FUNGAL DNA;

ANALYSIS; ASPERGILLUS; BERTHOLLETIA; CLASSIFICATION; EVALUATION STUDY; FOOD CONTAMINATION; FUNGUS SPORE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MAIZE; METABOLISM; MICROBIOLOGY; NUCLEIC ACID AMPLIFICATION; PEANUT; PROCEDURES; PRODUCT SAFETY;

AFLATOXINS; ARACHIS HYPOGAEA; ASPERGILLUS; BERTHOLLETIA; CONSUMER PRODUCT SAFETY; DNA, FUNGAL; FOOD CONTAMINATION; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SPORES, FUNGAL; ZEA MAYS;

EID: 84903144483     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2014.06.004     Document Type: Article

References (36)

1. Adams M., Motarjemi Y. World Health Organization WHO/SDE/PHE/FOS/99.1 1999.
2. Alary R., Serin A., Maury D., Ben Jouira H., Sirven J.P., Gautier M.F., Joudrier P. Comparison of simplex and duplex real-time PCR for the quantification of GMO in maize and soybean. Food Control 2002, 13:235-244.
3. Bernáldez V., Rodríguez A., Martín A., Lozano D., Córdoba J.J. Development of a multiplex qPCR method for simultaneous quantification in dry-cured ham of an antifungal-peptide Penicillium chrysogenum strain used as protective culture and aflatoxin-producing moulds. Food Control 2014, 36:257-265.
4. Calderari T.O., Iamanaka B.T., Frisvad J.C., Pitt J.I., Sartori D., Pereira J.L., Pelegrinelli Fungaro M.H., Taniwaki M.H. The biodiversity of Aspergillus section Flavi in Brazil nuts: from rainforest to consumer. Int. J. Food Microbiol. 2013, 160:267-272.
5. Cary J.W., Klich M.A., Beltz S.B. Characterization of aflatoxin-producing fungi outside of Aspergillus section Flavi. Mycologia 2005, 97:425-432.
6. Denschlag C., Vogel R.F., Niessen L. Hyd5 gene based analysis of cereals and malt for gushing-inducing Fusarium spp. by real-time LAMP using fluorescence and turbidity measurements. Int. J. Food Microbiol. 2013, 162:245-251.
7. Färber P., Geisen R., Holyapfel W.H. Detection of aflatoxigenic fungi in figs by PCR reaction. Int. J. Food Microbiol. 1997, 36:215-220.
8. Gunterus A., Roze L.V., Beaudry R., Linz J.E. Ethylene inhibits aflatoxin biosynthesis in Aspergillus parasiticus grown on peanuts. Food Microbiol. 2007, 24:658-663.
9. Haugland R.A., Brinkman N., Vesper S.J. Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis. J.Microbiol. Methods 2002, 50:319-323.
10. IARC International Agency for Research on Cancer: Monograph on the Evaluation of Carcinogenic Risk to Human 1993, vol. 56:257-263. Lyon, France.
11. Johnsson P., Lindblad M., Thim A.M., Jonsson N., Vargas E.A., Medeiros N.L., Brabet C., Olsen M. Growth of aflatoxigenic moulds and aflatoxin formation in Brazil nuts. World Mycotoxin J. 2008, 1:127-137.
12. Kumar V., Basu M.S., Rajendran T.P. Crop Prot. 2008, 27:891-905.
13. Lund F., Frisvad J.C. Penicillium verrucosum in wheat and barley indicates presence of ochratoxin A. J.Appl. Microbiol. 2003, 95:1117-1123.
14. Luo J., Vogel R.F., Niessen L. Development and application of a loop-mediated isothermal amplification assay for rapid identification of aflatoxigenic molds and their detection in food samples. Int. J. Food Microbiol. 2012, 159:214-224.
15. Luo J., Taniwaki M.H., Iaman B.T., Vogel R.F., Niessen L. Application of loop-mediated isothermal amplification assays for direct identification of pure cultures of Aspergillus flavus, A. nomius, and A.caelatus and for their rapid detection in shelled Brazil nuts. Int. J. Food Microbiol. 2014, 172:5-12.
16. Mayer Z., Bagnara A., Färber P., Geisen R. Quantification of the copy number of nor-1, a gene of the aflatoxin biosynthetic pathway by real-time PCR, and its correlation to the cfu of Aspergillus flavus in foods. Int. J. Food Microbiol. 2003, 82:143-151.
17. Mideros S.X., Windham G.L., Williams W.P., Nelson R.J. Aspergillus flavus biomass in maize estimated by quantitative real-time polymerase chain reaction is strongly correlated with aflatoxin concentration. Plant Dis. 2009, 93:1163-1170.
18. Niessen L., Vogel R.F. Detection of Fusarium graminearum DNA using a loop-mediated isothermal amplification (LAMP) assay. Int. J. Food Microbiol. 2010, 140:183-191.
19. Niessen L., Luo J., Denschlag C., Vogel R.F. The application of loop-mediated isothermal amplification (LAMP) in food testing for bacterial pathogens and fungal contaminants. Food Microbiol. 2013, 36:191-206.
20. Notermans S., Heuvelman C.J., Beumer R.R., Maas R. Immunological detection of moulds in food: relation between antigen production and growth. Int. J. Food Microbiol. 1986, 3:253-261.
21. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000, 28:e63. 10.1093/nar/28.12.e63.
22. Olsen M., Möller T., Thuvander A., Salmonsson A.-C. Mögelgifter-aflatoxiner och aflatocinproducerande mögelsvampar i kryddor-en riskvärdering. Vär Föda 1998, 4:33-35. (In Swedish).
23. Olsen M., Johnsson P., Moeller T., Paladino R., Lindblad M. Aspergillus nomius, an important aflatoxin producer in Brazil nuts?. World Mycotoxin J. 2008, 1:123-126.
24. Passone M.A., Rosso L.C., Ciancio A., Etcheverry M. Detection and quantification of Aspergillus section Flavi spp. in stored peanuts by real-time PCR of nor-1 gene, and effects of storage conditions on aflatoxin production. Int. J. Food Microbiol. 2010, 138:276-281.
25. Pildain M.B., Frisvad J.C., Vaamonde G., Cabral D., Varga J., Samson R.A. Two novel aflatoxin producing Aspergillus species from Argentinean peanuts. Int. J. Syst. Evol. Microbiol. 2008, 58:725-735.
26. Pitt J.I., Hocking A.A.D. Fungi and Food Spoilage 2009, 143-167. Academic Press, Sydney, Australia.
27. Rodríguez A., Rodríguez M., Andrade M.J., Córdoba J.J. Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods. Int. J. Food Microbiol. 2012, 155:10-18.
28. Rodríguez A., Rodríguez M., Martin A., Nuñez F., Cordoba J.J. Evaluation of hazard of aflatoxin B1, Ochratoxin A and patulin production in dry-cured ham and early detection of production of moulds by qPCR. Food Control 2012, 27:118-126.
29. ® Green quantitative PCR. Int. J. Food Microbiol. 2011, 145:121-125.
30. Shapira R., Paster N., Eyal O., Menasherov M., Mett A., Salomon R. Detection of aflatoxigenic molds in grains by PCR. Appl. Environ. Microbiol. 1996, 62:3270-3273.
31. Suanthie Y., Cousin M.A., Woloshuk C.P. Multiplex real-time PCR for detection and quantification of mycotoxigenic Aspergillus, Penicillium and Fusarium. J.Stored Prod. Res. 2009, 45:139-145.
32. Tombelli S., Mascini M., Scherm B., Battacone G., Migheli Q. DNA biosensors for the detection of aflatoxin producing Aspergillus flavus and A.parasiticus. Mon. Chem. Chem. Mon. 2009, 140:901-907.
33. Turner P., Sylla A., Gong Y., Diallo M., Sutcliffe A., Hall A., Wild C. Reduction in exposure to carcinogenic aflatoxins by postharvest intervention measures in west Africa: a community-based intervention study. Lancet 2005, 365:1950-1959.
34. Valasek M.A., Repa J.J. The power of real-time PCR. Adv. Physiol. Educ. 2005, 29:151-159.
35. van Egmond H.P., Jonker M.A. Worldwide Regulations for Mycotoxins in Food and Feed in 2003 2004, 165. Food and Agriculture Organization of the United Nations, Rome.
36. Varga J., Frisvad J.C., Samson R.A. Areappraisal of fungi producing aflatoxins. World Mycotoxin J. 2009, 2(3):263-277.