Direct affinity immobilization of recombinant heparinase I fused to maltose binding protein on maltose-coated magnetic nanoparticles

Biochemical Engineering Journal

Volumn 90, Issue , 2014, Pages 170-177

  Wu, Jingjun ^a   Zhou, Lilin ^a   Zhang, Huijuan ^a   Guo, Jun ^a   Mei, Xiang ^a   Zhang, Chong ^a   Yuan, Jinying ^a   Xing, Xin Hui ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Adsorption; Affinity; Enzyme biocatalysis; Heparinase I; Immobilization; Magnetic nanoparticles

Indexed keywords

ADSORPTION; BIOSYNTHESIS; ENZYME IMMOBILIZATION; MALTOSE; MOLECULAR WEIGHT DISTRIBUTION; NANOMAGNETICS; POLYETHYLENE OXIDES; POLYSACCHARIDES; RADIOACTIVE WASTE VITRIFICATION; RECOMBINANT PROTEINS; REUSABILITY;

ADSORPTION CAPACITIES; AFFINITY; ANTICOAGULANT ACTIVITIES; ENZYMATIC PRODUCTION; ENZYME BIOCATALYSIS; LOW MOLECULAR WEIGHT HEPARIN (LMWHS); MAGNETIC NANO-PARTICLES; MALTOSE BINDING PROTEINS;

NANOPARTICLES;

LOW MOLECULAR WEIGHT HEPARIN; MAGNETIC NANOPARTICLE; MALTOSE BINDING PROTEIN; RECOMBINANT HEPARINASE I; UNCLASSIFIED DRUG;

ADSORPTION; ANTICOAGULATION; ARTICLE; DEPOLYMERIZATION; IMMOBILIZATION; MOLECULAR WEIGHT; PRIORITY JOURNAL;

EID: 84903139918     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2014.05.021     Document Type: Article

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