Production of MBP-HepA fusion protein in recombinant Escherichia coli by optimization of culture medium

Biochemical Engineering Journal

Volumn 34, Issue 2, 2007, Pages 114-121

  Chen, Yin ^a   Xing, Xin Hui ^a   Ye, Fengchun ^a   Kuang, Ying ^a   Luo, Mingfang ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Fusion protein; HepA; MBP; Orthogonal experimental design; Recombinant Escherichia coli; Vector modification

Indexed keywords

FUSION PROTEIN; ORTHOGONAL EXPERIMENTAL DESIGN; RECOMBINANT ESCHERICHIA COLI; VECTOR MODIFICATION;

CELL CULTURE; DRUG PRODUCTS; ESCHERICHIA COLI; MALTOSE; MOLECULAR WEIGHT;

PROTEINS;

HEPARIN LYASE; HEPARINASE I; HYBRID PROTEIN; LOW MOLECULAR WEIGHT HEPARIN; MALTOSE BINDING PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM CULTURE; BIOREACTOR; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE OPTIMIZATION; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; EXPRESSION VECTOR; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS;

ESCHERICHIA COLI;

EID: 33947305443     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2006.11.020     Document Type: Article

References (27)

1. Galliher P.M., Cooney C.L., Langer R., and Linhardt R.J. Heparinase production by Flavobacterium heparinum. Appl. Environ. Microbiol. 41 (1981) 360-365
2. Ernst S., Rhomberg A.J., Biemann K., and Sasisekharan R. Direct evidence for a predominantly exolytic processive mechanism for depolymerization of heparin-like glycosaminoglycans by heparinase I. Proc. Natl. Acad. Sci. U.S.A. 95 (1998) 4182-4187
3. Langer R., Linhardt R.J., Hoffberg S., Larsen A.K., Cooney C.L., Tapper D., and Klein M. An enzymatic system for removing heparin in extracorporeal therapy. Science 217 (1982) 261-263
4. Yang V.C., Linhardt R.J., Bernstein H., Cooney C.L., and Langer R. Purification and characterization of heparinase from Flavobacterium heparinum. J. Biol. Chem. 260 (1985) 1849-1857
5. Sasisekharan R., Bulmer M., Moremen K.W., Cooney C.L., and Langer R. Cloning and expression of heparinase I gene from Flavobacterium heparinum. Proc. Natl. Acad. Sci. U.S.A. 90 (1993) 3660-3664
6. Ernst S., Venkataraman G., Winkler S., Godavarti R., Langer R., Cooney C.L., and Sasisekharan R. Expression in Escherichia coli, purification and characterization of heparinase I from Flavobacterium heparinum. Biochem. J. 315 (1996) 589-597
7. Shpigel E., Goldlust A., Efroni G., Avraham A., Eshel A., Dekel M., and Shoseyov O. Immobilization of recombinant heparinase I fused to cellulose-binding domain. Biotechnol. Bioeng. 65 (1999) 17-23
8. Chen Y., Xing X.H., and Lou K. Construction of recombinant Escherichia coli for over-production of soluble heparinase I by fusion to maltose-binding protein. Biochem. Eng. J. 23 (2005) 155-159
9. Kapust R.B., and Waugh D.S. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 8 (1999) 1668-1674
10. Kuang Y., Xing X.-H., Chen Y., Ye F.C., Chen Y., Yang Y.Y., Liu Z., and Bi R.C. Production of heparin oligosaccharides by fusion protein of MBP-heparinase I and the enzymatic thermostability. J. Mol. Catal. B: Enzym. 43 (2006) 90-95
11. Naal Z., Park J.H., Bernhard S., Shapleigh J.P., Batt C.A., and Abruna H.D. Amperometric TNT biosensor based on the oriented immobilization of a nitroreductase maltose binding protein fusion. Anal. Chem. 74 (2002) 140-148
12. Liu D., Shriver Z., Godavarti R., Venkataraman G., and Sasisekharan R. The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymatic activity. J. Biol. Chem. 274 (1999) 4089-4095
13. Silveira R.G., Kakizono T., Takemoto S., Nishio N., and Nagai S. Medium optimization by an orthogonal array design for the growth of Methanosarcina barkeri. J. Ferm. Bioeng. 72 (1991) 20-25
14. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31 (1959) 426-428
15. Sambrook J., and Russell D.W. Molecular Cloning. 3rd ed. (2001), Cold Spring Harbor, New York
16. Su H., Blain F., Musil R.A., Zimmermann J.J., Gu K., and Bennett D.C. Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum. Appl. Environ. Microbiol. 62 (1996) 2723-2734
17. Yeliseev A.A., Wong K.K., Soubias O., and Gawrisch K. Expression of human peripheral cannabinoid receptor for structural studies. Protein Sci. 14 (2005) 2638-2653
18. Weiss H.M., and Grisshammer R. Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli. Eur. J. Biochem. 269 (2002) 82-92
19. White J.F., Trinh L.B., Shiloach J., and Grisshammer R. Automated large-scale purification of a G protein-coupled receptor for neurotensin. FEBS Lett. 564 (2004) 289-293
20. Shriver Z., liu D., Hu Y., and Sasisekharan R. Biochemical investigations and mapping the calcium-binding sites of heparinase I from Flavobacterium heparinum. J. Biol. Chem. 274 (1999) 4082-4088
21. Watanabe M., Tsuda H., Yamada S., Shibata Y., Nakamura T., and Sugahara K. Characterization of heparinase from an oral bacterium Prevotella heparinolytica. J. Biochem. 123 (1998) 283-288
22. Kim B.T., Kim W.S., Kim Y.S., Linhardt R.J., and Kim D.H. Purification and characterization of a novel heparinase from Bacteroides stercoris HJ-15. J. Biochem. 128 (2000) 323-328
23. Shin C.S., Hong M.S., Bae C.S., and Lee J. Enhanced production of human mini-proinsulin in fed-batch cultures at high cell density of Escherichia coli BL21(DE3)[pET-3aT2M2]. Biotechnol. Prog. 13 (1997) 249-257
24. Lee C., Sun W.J., Burgess B.W., Junker B.H., Reddy J., Buckland B.C., and Greasham R.L. Process optimization for large-scale production of TGF-a-PE40 in recombinant Escherichia coli: effect of medium composition and induction timing on protein expression. J. Ind. Microbiol. Biotechnol. 18 (1997) 260-266
25. M.I. Viitanen, A. Vasala, P. Neubauer, T. Alatossava, Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli, Microbial Cell Factories 2:2, 2003.
26. Shiloach J., and Fass R. Growing E. coli to high cell density-a historical perspective on method development. Biotechnol. Adv. 23 (2005) 345-357
27. Lohse D.L., and Linhardt R.J. Purification and characterization of heparin lyases from Flavobacterium heparinum. J. Biol. Chem. 276 (1992) 24347-24355