Increased phosphorylation of ezrin is associated with the migration and invasion of fibroblast-like synoviocytes from patients with rheumatoid arthritis

Rheumatology (United Kingdom)

Volumn 53, Issue 7, 2014, Pages 1291-1300

  Xiao, Youjun ^a   Sun, Mengying ^a   Zhan, Zhongping ^a   Ye, Yujin ^a   Huang, Mingcheng ^a   Zou, Yaoyao ^a   Liang, Liuqin ^a   Yang, Xiuyan ^a   Xu, Hanshi ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

Ezrin; Fibroblast like synoviocytes; Invasion; Phosphorylation; Rheumatoid arthritis; Signal transduction

Indexed keywords

ACTIN; EZRIN; INTERLEUKIN 1BETA; MITOGEN ACTIVATED PROTEIN KINASE P38; RHO KINASE; SMALL INTERFERING RNA; TUMOR NECROSIS FACTOR ALPHA;

ACTIN FILAMENT; ADULT; AGED; ARTICLE; CELL INVASION; CELL MIGRATION; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; FEMALE; FIBROBLAST LIKE SYNOVIOCYTE; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; JOINT DESTRUCTION; MALE; MIDDLE AGED; OSTEOARTHRITIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RHEUMATOID ARTHRITIS; SYNOVIOCYTE; SYNOVIUM; WESTERN BLOTTING;

EZRIN; FIBROBLAST-LIKE SYNOVIOCYTES; INVASION; PHOSPHORYLATION; RHEUMATOID ARTHRITIS; SIGNAL TRANSDUCTION;

ADULT; ARTHRITIS, RHEUMATOID; CELL MOVEMENT; CELLS, CULTURED; CYTOSKELETAL PROTEINS; FEMALE; FIBROBLASTS; HUMANS; INTERLEUKIN-1BETA; MALE; MIDDLE AGED; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHORYLATION; RHO-ASSOCIATED KINASES; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; SYNOVIAL MEMBRANE; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84902995126     PISSN: 14620324     EISSN: 14620332     Source Type: Journal    
DOI: 10.1093/rheumatology/keu013     Document Type: Article

References (35)

1. Huber LC, Distler O, Tarner I et al. Synovial fibroblasts: key players in rheumatoid arthritis. Rheumatology 2006; 45:669-75.
2. Pap T, Muller-Ladner U, Gay RE, Gay S. Fibroblast biology:role of synovial fibroblast in the pathogenesis of rheumatoid arthritis. Arthritis Res 2000; 2:361-7.
3. Firestein GS, Zvaifler NJ. How important are T cells in chronic rheumatoid synovitis?: II. T cell-independent mechanisms from begin to end. Arthritis Rheum 2002; 46:298-308.
4. Müller-Ladner U, Kriegsmann J, Franklin BN et al. Synovial fibroblasts of patients with rheumatoid arthritis attach to and invade normal human cartilage when engrafted into SCID mice. Am J Pathol 1996; 149:1607-15.
5. Lefèvre S, Knedla A, Tennie C et al. Synovial fibroblasts spread rheumatoid arthritis to unaffected joints. Nat Med 2009; 15:1414-20.
6. Pap T, Meinecke I, Muller-Ladner U, Gay S. Are fibroblasts involved in joint destruction? Ann Rheum Dis 2005; 64(Suppl 4):iv52-4.
7. Smith CA. Properties of synovial cells in culture. J Exp Med 1971;134:306s-12.
8. Firestein GS, Echeverri F, Yeo M, Zvaifler NJ, Green DR. Somatic mutations in the p53 tumor suppressor gene in rheumatoid arthritis synovium. Proc Natl Acad Sci USA 1997; 94:10895-900.
9. Roivainen A, Jalava J, Pirila L et al. H-ras oncogene point mutation in arthritic synovium. Arthritis Rheum 1997; 40:1636-43.
10. Bretscher A, Reczek D, Berryman M. Ezrin: a protein requiring conformational activation to link microfilaments to the plasma membrane in the assembly of cell surface structures. J Cell Sci 1997; 110:3011-18.
11. Louver-Vallee S. ERM proteins: from cellular architecture to cell signaling. Biol Cell 2000; 92:305-16.
12. Saotome I, Curto M, McClatchey AI. Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine. Dev Cell 2004; 6:855-64.
13. Faure S, Salazar-Fontana LI, Semichon M et al. ERM proteins regulate cytoskeleton relaxation promoting T cell-APC conjugation. Nat Immunol 2004; 5:272-79.
14. Bretscher A, Edwards K, Fehon RG. ERM proteins and merlin: integrators at the cell cortex. Nat Rev Mol Cell Biol 2002; 3:586-99.
15. Yu Y, Khan J, Khanna C et al. Expression profiling identifies the cytoskeletal organizer ezrin and the developmental homeoprotein Six-1 as key metastatic regulators. Nat Med 2004; 10:175-81.
16. Khanna C, Wan X, Bose S et al. The membranecytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat Med 2004; 10:182-6.
17. Arnett FC, Edworthy SM, Bloch DA et al. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 1988; 31:315-24.
18. Altman R, Asch E, Bloch D et al. Development of criteria for the classification and reporting of osteoarthritis: classification of osteoarthritis of the knee. Arthritis Rheum 1986; 29:1039-49.
19. Tolboom TC, van der Helm-Van Mil AH, Nelissen RG et al. Invasiveness of fibroblast-like synoviocytes is an individual patient characterize associated with the rate of joint destruction in patients with rheumatoid arthritis. Arthritis Rheum 2005; 52:1999-2002.
20. Müller-Ladner U, Pap T, Gay RE, Neidhart M, Gay S. Mechanisms of disease: the molecular and cellular basis of joint destruction in rheumatoid arthritis. Nat Clin Pract Rheumatol 2005; 1:102-10.
21. Volin MV, Huynh N, Klosowska K, Chong KK, Woods JM. Fractalkine is a novel chemoattractant for rheumatoid arthritis fibroblast-like synoviocyte signaling through MAP kinases and Akt. Arthritis Rheum 2007; 56:2512-22.
22. Gravallese EM, Manning C, Tsay A et al. Synovial tissue in rheumatoid arthritis is a source of osteoclast differentiation factor. Arthritis Rheum 2000; 43:250-8.
23. Mak H, Naba A, Varma S et al. Ezrin phosphorylation on tyrosine 477 regulates invasion and metastasis of breast cancer cells. BMC Cancer 2012; 12:82.
24. Oda Y, Aishima S, Morimatsu K et al. Differential ezrin and phosphorylated ezrin expression profiles between pancreatic intraepithelial neoplasia, intraductal papillary mucinous neoplasm, and invasive ductal carcinoma of the pancreas. Hum Pathol 2013; 44:1487-98.
25. Weng WH, Yeh CN, Cheng YF, Lenka G, Liaw YW. Phosphorylated T567 ezrin is associated with merlin expression in KIT-mutant gastrointestinal stromal tumors. Mol Med Rep 2012; 5:17-21.
26. Ren L, Hong SH, Chen QR et al. Dysregulation of ezrin phosphorylation prevents metastasis and alters cellular metabolism in osteosarcoma. Cancer Res 2012; 72:1001-2.
27. Agbor TA, Demma ZC, Mumy KL, Bien JD, McCormick BA. The ERM protein, ezrin, regulates neutrophil transmigration by modulating the apical localization of MRP2 in response to the SipA effector protein during salmonella typhimurium infection. Cell Microbiol 2011; 13:2007-21.
28. Parameswaran N, Enyindah-Asonye G, Bagheri N, Shah NB, Gupta N. Spatial coupling of JNK activation to the B cell antigen receptor by tyrosine-phosphorylated ezrin. J Immunol 2013; 190:2017-26.
29. Laragione T, Gulko PS. mTOR regulates the invasive properties of synovial fibroblasts in rheumatoid arthritis. Mol Med 2010; 16:352-8.
30. Yasui Y, Amano M, Nagata K et al. Roles of Rhoassociated kinase in cytokinesis; mutations in Rhoassociated kinase phosphorylation sites impair cytokinetic segregation of glial filaments. J Cell Biol 1998; 143:1249-58.
31. Aidinis V, Carninci P, Armaka M et al. Cytoskeletal rearrangements in synovial fibroblasts as a novel determinant of modeled rheumatoid arthritis. PLoS Genet 2005;1:e48.
32. Vasilopoulos Y, Gkretsi V, Armaka M, Aidinis V, Kollias G. Actin cytoskeleton dynamics linked to synovial fibroblast activation as a novel pathogenic principle in TNF-driven arthritis. Ann Rheum Dis 2007;66(Suppl III):iii23-8.
33. Hu Z, Wang Y, Graham WV et al. MAPKAPK-2 is a critical signaling intermediate in NHE3 activation following Na+-glucose cotransport. J Biol Chem 2006; 281:24247-53.
34. Wakayama Y, Miura K, Sabe H, Mochizuki N. EphrinA1-EphA2 signal induces compaction and polarization of Madin-Darby canine kidney cells by inactivating ezrin through negative regulation of RhoA. J Biol Chem 2011; 286:44243-53.
35. Tang F, Zou F, Peng Z et al. N,N0-dinitrosopiperazinemediated ezrin phosphorylation via activating Rho kinase and protein kinase C involves in metastasis of nasopharyngeal carcinoma 6-10B cells. J Biol Chem 2011; 286:36956-67.