Scientific Opinion on VTEC-seropathotype and scientific criteria regarding pathogenicity assessment

EFSA Journal

Volumn 11, Issue 4, 2013, Pages

[No Author Info available]

Author keywords

detection; identification; isolation; serogroup; seropathotype; virulence factors; VTEC

Indexed keywords

EID: 84902983303     PISSN: None     EISSN: 18314732     Source Type: Journal    
DOI: 10.2903/j.efsa.2013.3138     Document Type: Article

References (117)

1. Abadias M, Usall J, Anguera M, Solson C and Vinas I, 2008. Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments. International Journal of Food Microbiology, 123, 121–129.
2. Althaus D, Hofer E, Corti S, Julmi A and Stephan R, 2012. Bacteriological survey of ready-to-eat lettuce, fresh-cut fruit, and sprouts collected from the Swiss market. Journal of Food Protection, 75, 1338–1341.
3. Arthur L, Jones S, Fabri M and Odumeru J, 2007. Microbial survey of selected Ontario-grown fresh fruits and vegetables. Journal of Food Protection, 70, 2864–2867.
4. Barrett TJ, Lior H, Green JH, Khakhria R, Wells JG, Bell BP, Greene KD, Lewis J and Griffin PM, 1994. Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel-electrophoresis and phage typing. Journal of Clinical Microbiology, 32, 3013–3017.
5. Bettelheim KA, 2007. The non-O157 Shiga-toxigenic (verocytotoxigenic) Escherichia coli; underrated pathogens. Critical Reviews in Microbiology, 33, 67–87.
6. Bettelheim KA, 2012. Non-O157 Shiga-toxin-producing Escherichia coli. Lancet Infectious Diseases, 12, 12–12.
7. Bielaszewska M, Friedrich AW, Aldick T, Schuerk-Bulgrin R and Karch H, 2006. Shiga toxin activatable by intestinal mucus in Escherichia coli isolated from humans: Predictor for a severe clinical outcome. Clinical Infectious Diseases, 43, 1160–1167.
8. Blanco J, Blanco M, Blanco J, Mora A, Alonso M, Gonzalez E and Bernardez M, 2001. Epidemiology of verocytotoxigenic Escherichia coli (VTEC) in ruminants. In: Verocytotoxigenic E. coli. Eds Duffy G, Garvey P, McDowell DA, Food & Nutrition Press, Inc., Trumbull, Connecticut, USA, 113–148.
9. Bohaychuk VM, Bradbury RW, Dimock R, Fehr M, Gensler GE, King RK, Rieve R and Romero Barrios P, 2009. A microbiological survey of selected Alberta-grown fresh produce from farmers' markets in Alberta, Canada. Journal of Food Protection, 72, 415–420.
10. Bonardi S, Foni E, Chiapponi C, Salsi A and Brindani F, 2007. Detection of verocytotoxin-producing Escherichia coli serogroups O157 and O26 in the cecal content and lymphatic tissue of cattle at slaughter in Italy. Journal of Food Protection, 70, 1493–1497.
11. Bosilevac JM and Koohmaraie M, 2011. Prevalence and characterization of non-O157 Shiga toxin-producing Escherichia coli isolates from commercial ground beef in the United States. Applied and Environmental Microbiology, 77, 2103–2112.
12. Brooks JT, Sowers EG, Wells JG, Greene KD, Griffin PM, Hoekstra RM and Strockbine NA, 2005. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983–2002. Journal of Infectious Diseases, 192, 1422–1429.
13. Buchholz U, Bernard H, Werber D, Bohmer MM, Remschmidt C, Wilking H, Delere Y, an der Heiden M, Adlhoch C, Dreesman J, Ehlers J, Ethelberg S, Faber M, Frank C, Fricke G, Greiner M, Hohle M, Ivarsson S, Jark U, Kirchner M, Koch J, Krause G, Luber P, Rosner B, Stark K and Kuhne M, 2011. German outbreak of Escherichia coli O104:H4 associated with sprouts. New England Journal of Medicine, 365, 1763–1770.
14. Caprioli A, Tozzi AE, Rizzoni G and Karch H, 1997. Non-O157 Shiga toxin-producing Escherichia coli infections in Europe. Emerging Infectious Diseases, 3, 578–579.
15. Castro-Rosas J, Cerna-Cortes JF, Mendez-Reyes E, Lopez-Hernandez D, Gomez-Aldapa CA and Estrada-Garcia T, 2012. Presence of faecal coliforms, Escherichia coli and diarrheagenic E. coli pathotypes in ready-to-eat salads, from an area where crops are irrigated with untreated sewage water. International Journal of Food Microbiology, 156, 176–180.
16. Clements A, Young JC, Constantinou N and Frankel G, 2012. Infection strategies of enteric pathogenic Escherichia coli. Gut microbes, 3, 71–87.
17. Coombes BK, Gilmour MW and Goodman CD, 2011. The evolution of virulence in non-O157 Shiga toxin-producing Escherichia coli. Frontiers in Microbiology, 2, 1–3.
18. Coombes BK, Wickham ME, Mascarenhas M, Gruenheid S, Finlay BB and Karmali MA, 2008. Molecular analysis as an aid to assess the public health risk of non-O157 Shiga toxin-producing Escherichia coli strains. Applied and Environmental Microbiology, 74, 2153–2160.
19. De Giusti M, Aurigemma C, Marinelli L, Tufi D, De Medici D, Di Pasquale S, De Vito C and Boccia A, 2010. The evaluation of the microbial safety of fresh ready-to-eat vegetables produced by different technologies in Italy. Journal of Applied Microbiology, 109, 996–1006.
20. DeGrandis S, Law H, Brunton J, Gyles C and Lingwood CA, 1989. Globotetraosylceramide is recognized by the pig edema disease toxin. The Journal of Biological Chemistry, 264, 12520–12525.
21. Devakumar D, Kitching A, Zenner D, Tostmann A and Meltzer M, 2013. Tracking sickness through social networks - the practical use of social network mapping in supporting the management of an E. coli O157 outbreak in a primary school in London. Epidemiology and Infection, doi:10.1017/S0950268813000344.
22. Dudley EG, Thomson NR, Parkhill J, Morin NP and Nataro JP, 2006. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Molecular Microbiology, 61, 1267–1282.
23. EC (European Commission Health & Consumer Protection Directorate-General, Directorate C), 2003. Opinion of the Scientific Committee on veterinary measures relating to public health on verotoxigenic E. coli (VTEC) in foodstuffs. European Commission Health & Consumer Protection Directorate-General, Directorate C.
24. EC (European Commission. DG SANCO), 2012. Surveillance of enteric pathogens in Europe and beyond. International surveillance network for the enteric infections - Salmonella, VTEC O157 and Campylobacter. Annual report 2007. European Commission. Report No: 1786/2002/EC, 197 pp.
25. ECDC (European Centre for Disease Prevention and Control), 2011. Methodology protocol for estimating burden of communicable diseases. 43. www.ecdc.europa.eu/en/publications/Publications/Forms/ECDC_DispForm.aspx?ID=688.
26. EFSA (European Food Safety Authority), 2007. Scientific Opinion of the Panel on Biological Hazards (BIOHAZ) on monitoring of verotoxigenic Escherichia coli (VTEC) and identification of human pathogenic VTEC types. The EFSA Journal, 579, 1–61.
27. EFSA (European Food Safety Authority), 2009. Scientific report on technical specifications for the monitoring and reporting of verotoxigenic Escherichia coli (VTEC) on animals and food (VTEC surveys on animals and food). EFSA Journal, 7(11):1366, 43 pp.
28. EFSA and ECDC (European Food Safety Authority, European Centre for Disease Prevention and Control), 2010. The Community Summary Report on Trends and sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in the European Union in 2008. EFSA Journal, 8(1):1496, 363 pp.
29. EFSA and ECDC (European Food Safety Authority, European Centre for Disease Prevention and Control), 2011. The European Union Summary Report on Trends and sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2009. EFSA Journal, 9(3):2090, 378 pp.
30. EFSA and ECDC (European Food Safety Authority, European Centre for Disease Prevention and Control), 2012. The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2010. EFSA Journal, 10(3):2597, 442 pp.
31. EFSA and ECDC (European Food Safety Authority, European Centre for Disease Prevention and Control), 2013. The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2011. EFSA Journal, 11(4):3129, 250 pp.
32. EFSA Panel on Biological Hazards (BIOHAZ), 2012a. Scientific Opinion on a review on the European Union Summary Reports on trends and sources zoonoses, zoonotic agents and food-borne outbreaks in 2009 and 2010 – specifically for the data on Salmonella, Campylobacter, verotoxigenic Escherichia coli, Listeria monocytogenes and foodborne outbreak. EFSA Journal, 10(6):2726, 25 pp.
33. EFSA Panel on Biological Hazards (BIOHAZ), 2012b. Scientific Opinion on an estimation of the public health impact of setting a new target for the reduction of Salmonella in turkeys. EFSA Journal, 10(3):2616, 89 pp.
34. EFSA Panel on Biological Hazards (BIOHAZ), 2012c. Scientific Opinion on Campylobacter in broiler meat production: control options and performance objectives and/or targets at different stages of the food chain. EFSA Journal, 9(4):2105, 141 pp.
35. EFSA Panel on Biological Hazards (BIOHAZ), 2013. Scientific Opinion on the risk posed by pathogens in food of non-animal origin. Part 1 (outbreak data analysis and risk ranking of food/pathogen combinations). EFSA Journal, 11(1):3025, 138 pp.
36. Ennis C, McDowell DA and Bolton DJ, 2012. The prevalence, distribution and characterisation of Shiga toxin-producing Escherichia coli (STEC) serotypes and virulotypes from a cluster of bovine farms. Journal of Applied Microbiology, 113, 1238–1248.
37. Ethelberg S, Olsen KEP, Scheutz F, Jensen C, Schiellerup P, Engberg J, Petersen AM, Olesen B, Gerner-Smidt P and Molbak K, 2004. Virulence factors for hemolytic uremic syndrome, Denmark. Emerging Infectious Diseases, 10, 842–847.
38. Fitzpatrick M, 1999. Haemolytic uraemic syndrome and E. coli O157. British Medical Journal, 318, 684–685.
39. Frank C, Faber MS, Askar M, Bernard H, Fruth A, Gilsdorf A, Hohle M, Karch H, Krause G, Prager R, Spode A, Stark K, Werber D and Team HUSI, 2011a. Large and ongoing outbreak of haemolytic uraemic syndrome, Germany, May 2011. Eurosurveillance, 16, 2–4.
40. Frank C, Werber D, Cramer JP, Askar M, Faber M, an der Heiden M, Bernard H, Fruth A, Prager R, Spode A, Wadl M, Zoufaly A, Jordan S, Kemper MJ, Follin P, Muller L, King LA, Rosner B, Buchholz U, Stark K, Krause G and Team HUSI, 2011b. Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in Germany - preliminary report. The New England Journal of Medicine, 365, 1771–1780.
41. Fratamico PM, Bagi LK, Cray WCJr., Narang N, Yan X, Medina M and Liu Y, 2011. Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef. Foodborne Pathogens and Disease, 8, 601–607.
42. Friedrich AW, Bielaszewska M, Zhang WL, Pulz M, Kuczius T, Ammon A and Karch H, 2002. Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. The Journal of Infectious Diseases, 185, 74–84.
43. Gillespie I, Little C and Mitchell R, 2000. Microbiological examination of cold ready-to-eat sliced meats from catering establishments in the United Kingdom. Journal of Applied Microbiology, 88, 467–474.
44. Gomez-Govea M, Solis-Soto L, Heredia N, Garcia S, Moreno G, Tovar O and Isunza G, 2012. Analysis of microbial contamination levels of fruits and vegetables at retail in Monterrey, Mexico. Journal of Food Agriculture & Environment, 10, 152–156.
45. Gould LH, Bopp C, Strockbine N, Atkinson R, Baselski V, Body B, Carey R, Crandall C, Hurd S, Kaplan R, Neill M, Shea S, Somsel P, Tobin-D'Angelo M, Griffin PM and Gerner-Smidt P, 2009. Recommendations for diagnosis of Shiga toxin-producing Escherichia coli infections by clinical laboratories. MMWR. Recommendations and reports: Morbidity and mortality weekly report. Recommendations and reports. Centers for Disease Control, 58, 1–14.
46. Gyles CL, 2007. Shiga toxin-producing Escherichia coli: An overview. Journal of Animal Science, 85, E45–E62.
47. Haagsma JA, Geenen PL, Ethelberg S, Fetsch A, Hansdotter F, Jansen A, Korsgaard H, O'Brien SJ, Scavia G, Spitznagel H, Stefanoff P, Tam CC and Havelaar AH, 2012. Community incidence of pathogen-specific gastroenteritis: reconstructing the surveillance pyramid for seven pathogens in seven European Union member states. Epidemiology and Infection, DOI: 10.1017/S0950268812002166.
48. Havelaar AH, Haagsma JA, Mangen MJJ, Kemmeren JM, Verhoef LPB, Vijgen SMC, Wilson M, Friesema IHM, Kortbeek LM, Van Duynhoven Y and Van Pelt W, 2012. Disease burden of foodborne pathogens in the Netherlands, 2009. International Journal of Food Microbiology, 156, 231–238.
49. Havelaar AH, Ivarsson S, Lofdahl M and Nauta MJ, 2013. Estimating the true incidence of campylobacteriosis and salmonellosis in the European Union, 2009. Epidemiology and Infection, 141, 293–302.
50. Hussein HS, 2007. Prevalence and pathogenicity of Shiga toxin-producing Escherichia coli in beef cattle and their products. Journal of Animal Science, 85, E63–E72.
51. Hyytia-Trees E, Smole SC, Fields PA, Swaminathan B and Ribot EM, 2006. Second generation subtyping: A proposed PulseNet protocol for multiple-locus variable-number tandem repeat analysis of Shiga toxin-producing Escherichia coli O157 (STEC O157). Foodborne Pathogens and Disease, 3, 118–131.
52. Imamovic L, Tozzoli R, Michelacci V, Minelli F, Marziano ML, Caprioli A and Morabito S, 2010. OI-57, a genomic island of Escherichia coli O157, is present in other seropathotypes of Shiga toxin-producing E. coli associated with severe human disease. Infection and Immunity, 78, 4697–4704.
53. Jacewicz M, Clausen H, Nudelman E, Donohue-Rolfe A and Keusch GT, 1986. Pathogenesis of Shigella diarrhea. XI. Isolation of a Shigella toxin-binding glycolipid from rabbit jejunum and HeLa-cells and its identification as globotriaosylceramide. Journal of Experimental Medicine, 163, 1391–1404.
54. Jenkins C, Lawson AJ, Cheasty T and Willshaw GA, 2012. Assessment of a real-time PCR for the detection and characterization of verocytotoxigenic Escherichia coli. Journal of medical microbiology, 61, 1082–1085.
55. Johannessen GS, Loncarevic S and Kruse H, 2002. Bacteriological analysis of fresh produce in Norway. International Journal of Food Microbiology, 77, 199–204.
56. Johnson KE, Thorpe CM and Sears CL, 2006. The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clinical Infectious Diseases, 43, 1587–1595.
57. Johnston LM, Jaykus LA, Moll D, Anciso J, Mora B and Moe CL, 2006. A field study of the microbiological quality of fresh produce of domestic and Mexican origin. International Journal of Food Microbiology, 112, 83–95.
58. Johnston LM, Jaykus LA, Moll D, Martinez MC, Anciso J, Mora B and Moe CL, 2005. A field study of the microbiological quality of fresh produce. Journal of Food Protection, 68, 1840–1847.
59. Kalchayanand N, Arthur TM, Bosilevac JM, Wells JE and Wheeler TL, 2013. Chromogenic agar medium for detection and isolation of Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 from fresh beef and cattle feces. Journal of Food Protection, 76, 192–199.
60. Kaper JB, Nataro JP and Mobley HLT, 2004. Pathogenic Escherichia coli. Nature Reviews Microbiology, 2, 123–140.
61. Karch H, Denamur E, Dobrindt U, Finlay BB, Hengge R, Johannes L, Ron EZ, Tonjum T, Sansonetti PJ and Vicente M, 2012. The enemy within us: lessons from the 2011 European Escherichia coli O104:H4 outbreak. Embo Molecular Medicine, 4, 841–848.
62. Karmali MA, Mascarenhas M, Shen SH, Ziebell K, Johnson S, Reid-Smith R, Isaac-Renton J, Clarks C, Rahn K and Kaper JB, 2003. Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease. Journal of Clinical Microbiology, 41, 4930–4940.
63. Khakhria R, Duck D and Lior H, 1990. Extended phage-typing scheme for Escherichia coli O157:H7. Epidemiology and Infection, 105, 511–520.
64. Koseki S, Mizuno Y, Kawasaki S and Yamamoto K, 2011. A survey of iceberg lettuce for the presence of Salmonella, Escherichia coli O157:H7, and Listeria monocytogenes in Japan. Journal of Food Protection, 74, 1543–1546.
65. Lindberg AA, Brown JE, Stromberg N, Westlingryd M, Schultz JE and Karlsson KA, 1987. Identification of the carbohydrate receptor for Shiga toxin produced by Shigella dysenteriae type-1. The Journal of Biological Chemistry, 262, 1779–1785.
66. Little CL and De Louvois J, 1998. The microbiological examination of butchery products and butchers' premises in the United Kingdom. Journal of Applied Microbiology, 85, 177–186.
67. Locking ME, Pollock KGJ, Allison LJ, Rae L, Hanson MF and Cowden JM, 2011. Escherichia coli O157 infection and secondary spread, Scotland, 1999–2008. Emerging Infectious Diseases, 17, 524–527.
68. Loncarevic S, Johannessen GS and Rorvik LM, 2005. Bacteriological quality of organically grown leaf lettuce in Norway. Letters in Applied Microbiology, 41, 186–189.
69. Lynn RM, O'Brien SJ, Taylor CM, Adak GK, Chart H, Cheasty T, Coia JE, Gillespie IA, Locking ME, Reilly WJ, Smith HR, Waters A and Willshaw GA, 2005. Childhood hemolytic uremic syndrome, United Kingdom and Ireland. Emerging Infectious Diseases, 11, 590–596.
70. Mauro SA and Koudelka GB, 2011. Shiga toxin: expression, distribution, and its role in the environment. Toxins, 3, 608–625.
71. Mellmann A, Bielaszewska M, Kock R, Friedrich AW, Fruth A, Middendorf B, Harmsen D, Schmidt MA and Karch H, 2008. Analysis of collection of hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli. Emerging Infectious Diseases, 14, 1287–1290.
72. Mellmann A, Harmsen D, Cummings CA, Zentz EB, Leopold SR, Rico A, Prior K, Szczepanowski R, Ji YM, Zhang WL, McLaughlin SF, Henkhaus JK, Leopold B, Bielaszewska M, Prager R, Brzoska PM, Moore RL, Guenther S, Rothberg JM and Karch H, 2011. Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology. PLoS One, 6.
73. Miko A, Lindstedt B-A, Brandal LT, Lobersli I and Beutin L, 2010. Evaluation of multiple-locus variable number of tandem-repeats analysis (MLVA) as a method for identification of clonal groups among enteropathogenic, enterohaemorrhagic and avirulent Escherichia coli O26 strains. Fems Microbiology Letters, 303, 137–146.
74. Monaghan A, Byrne B, Fanning S, Sweeney T, McDowell D and Bolton DJ, 2011. Serotypes and virulence profiles of non-O157 Shiga toxin-producing Escherichia coli isolates from bovine farms. Applied and Environmental Microbiology, 77, 8662–8668.
75. Monaghan A, Byrne B, Fanning S, Sweeney T, McDowell D and Bolton DJ, 2012. Serotypes and virulotypes of non-O157 shiga-toxin producing Escherichia coli (STEC) on bovine hides and carcasses. Food Microbiology, 32, 223–229.
76. Mora A, Leon SL, Blanco M, Blanco JE, Lopez C, Dahbi G, Echeita A, Gonzalez EA and Blanco J, 2007. Phage types, virulence genes and PFGE profiles of Shiga toxin-producing Escherichia coli O157:H7 isolated from raw beef, soft cheese and vegetables in Lima (Peru). International Journal of Food Microbiology, 114, 204–210.
77. Morabito S, Tozzoli R, Oswald E and Caprioli A, 2003. A mosaic pathogenicity island made up of the locus of enterocyte effacement and a pathogenicity of Escherichia coli O157:H7 is frequently present in attaching and effacing E. coli. Infection and Immunity, 71, 3343–3348.
78. Nataro JP and Kaper JB, 1998. Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11, 142–201.
79. Nataro JP, Steiner T and Guerrant RL, 1998. Enteroaggregative Escherichia coli. Emerging Infectious Diseases, 4, 251–261.
80. Noller AC, McEllistrem MC, Stine OC, Morris JG, Boxrud DJ, Dixon B and Harrison LH, 2003. Multilocus sequence typing reveals a lack of diversity among Escherichia coli O157:H7 isolates that are distinct by pulsed-field gel electrophoresis. Journal of Clinical Microbiology, 41, 675–679.
81. O'Brien AD, Karmali MA and Scotland SM, 1994. A proposal for rationalization of the Escherichia coli cytotoxins. In: Recent advances in verocytotoxin-producing Escherichia coli infections. Elsevier Science, B.V., Amsterdam, the Netherlands, 147–149.
82. OIE (World Organisation for Animal Health), 2008. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees). Chapter 2.9.11. Verocytotoxigenic Escherichia coli. World Organisation for Animal Health, 1294–1304 pp.
83. Oliveira M, Usall J, Vinas I, Anguera M, Gatius F and Abadias M, 2010. Microbiological quality of fresh lettuce from organic and conventional production. Food Microbiology, 27, 679–684.
84. Pennington TH, 2000. VTEC: lessons learned from British outbreaks. Journal of Applied Microbiology, 88, 90S–98S.
85. Persson S, Olsen KEP, Ethelberg S and Scheutz F, 2007. Subtyping method for Escherichia coli Shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations. Journal of Clinical Microbiology, 45, 2020–2024.
86. Pierard D, Huyghens L and Lauwers S, 1991. Diarrhea associated with Escherichia coli producing porcine edema disease verotoxin. Lancet, 338, 762–762.
87. Possé B, De Zutter L, Heyndrickx M and Herman L, 2008. Novel differential and confirmation plating media for Shiga toxin-producing Escherichia coli serotypes O26, O103, O111, O145 and sorbitolpositive and -negative O157. Fems Microbiology Letters, 282, 124–131.
88. Rasko DA, Webster DR, Sahl JW, Bashir A, Boisen N, Scheutz F, Paxinos EE, Sebra R, Chin CS, Iliopoulos D, Klammer A, Peluso P, Lee L, Kislyuk AO, Bullard J, Kasarskis A, Wang S, Eid J, Rank D, Redman JC, Steyert SR, Frimodt-Moller J, Struve C, Petersen AM, Krogfelt KA, Nataro JP, Schadt EE and Waldor MK, 2011. Origins of the E. coli strain causing an outbreak of Hemolytic-Uremic Syndrome in Germany. New England Journal of Medicine, 365, 709–717.
89. Roldgaard BB, Scheutz F, Boel J, Aabo S, Schultz AC, Cheasty T, Nielsen EM, Olsen KEP and Christensen BB, 2004. VTEC O157 subtypes associated with the most severe clinical symptoms in humans constitute a minor part of VTEC O157 isolates from Danish cattle. International Journal of Medical Microbiology, 294, 255–259.
90. Sagoo SK, Little CL and Mitchell RT, 2001. The microbiological examination of ready-to-eat organic vegetables from retail establishments in the United Kingdom. Letters in Applied Microbiology, 33, 434–439.
91. Sagoo SK, Little CL and Mitchell RT, 2003a. Microbiological quality of open ready-to-eat salad vegetables: Effectiveness of food hygiene training of management. Journal of Food Protection, 66, 1581–1586.
92. Sagoo SK, Little CL, Ward L, Gillespie IA and Mitchell RT, 2003b. Microbiological study of ready-to-eat salad vegetables from retail establishments uncovers a national outbreak of salmonellosis. Journal of Food Protection, 66, 403–409.
93. Samuel JE, Perera LP, Ward S, Obrien AD, Ginsburg V and Krivan HC, 1990. Comparison of the glycolipid receptor specificities of Shiga-like toxin type-II and Shiga-like toxin type-II variants. Infection and Immunity, 58, 611–618.
94. Santos MI, Cavaco A, Gouveia J, Novais MR, Nogueira PJ, Pedroso L and Ferreira M, 2012. Evaluation of minimally processed salads commercialized in Portugal. Food Control, 23, 275–281.
95. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, Jones JL and Griffin PM, 2011. Foodborne illness acquired in the United States - major pathogens. Emerging Infectious Diseases, 17, 7–15.
96. Scheutz F and Ethelberg S (Statens Serum Institut), 2008. Nordic Meeting on detection and surveillance of VTEC infections in humans.
97. Scheutz F, Teel LD, Beutin L, Pierard D, Buvens G, Karch H, Mellmann A, Caprioli A, Tozzoli R, Morabito S, Strockbine NA, Melton-Celsa AR, Sanchez M, Persson S and O'Brien AD, 2012. Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature. Journal of Clinical Microbiology, 50, 2951–2963.
98. Schimmer B, Nygard K, Eriksen HM, Lassen J, Lindstedt BA, Brandal LT, Kapperud G and Aavitsland P, 2008. Outbreak of haemolytic uraemic syndrome in Norway caused by stx(2)-positive Escherichia coli O103:H25 traced to cured mutton sausages. Bmc Infectious Diseases, 8, 41.
99. Sekse C, O'Sullivan K, Granum PE, Rorvik LM, Wasteson Y and Jorgensen HJ, 2009. An outbreak of Escherichia coli O103:H25 - Bacteriological investigations and genotyping of isolates from food. International Journal of Food Microbiology, 133, 259–264.
100. Tam CC, O'Brien SJ, Tompkins DS, Bolton FJ, Berry L, Dodds J, Choudhury D, Halstead F, Iturriza-Gomara M, Mather K, Rait G, Ridge A, Rodrigues LC, Wain J, Wood B, Gray JJ and Comm IIDSE, 2012. Changes in causes of acute gastroenteritis in the United Kingdom over 15 years: Microbiologic findings from 2 prospective, population-based studies of infectious intestinal disease. Clinical Infectious Diseases, 54, 1275–1286.
101. Taniuchi M, Walters CC, Gratz J, Maro A, Kumburu H, Serichantalergs O, Sethabutr O, Bodhidatta L, Kibiki G, Toney DM, Berkeley L, Nataro JP and Houpt ER, 2012. Development of a multiplex polymerase chain reaction assay for diarrheagenic Escherichia coli and Shigella spp. and its evaluation on colonies, culture broths, and stool. Diagnostic Microbiology and Infectious Disease, 73, 121–128.
102. Thomas A, Cheasty T, Chart H and Rowe B, 1994. Isolation of verocytotoxin-producing Escherichia coli serotypes O9ab:H- and O101:H carrying VT2 variant gene-sequences from a patient with hemolytic-uraemic-syndrome. European Journal of Clinical Microbiology & Infectious Diseases, 13, 1074–1076.
103. Tillman GE, Wasilenko JL, Simmons M, Lauze TA, Minicozzi J, Oakley BB, Narang N, Fratamico P and Cray WC, 2012. Isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 from ground beef using Modified Rainbow Agar and postimmunomagnetic separation acid treatment. Journal of Food Protection, 75, 1548–1554.
104. Todd WTA and Dundas S, 2001. The management of VTEC O157 infection. International Journal of Food Microbiology, 66, 103–110.
105. Tompkins DS, Hudson MJ, Smith HR, Eglin RP, Wheeler JG, Brett MM, Owen RJ, Brazier JS, Cumberland P, King V and Cook PE, 1999. A study of infectious intestinal disease in England: microbiological findings in cases and controls. Communicable Disease and Public Health, 2, 108–113.
106. Tozzi AE, Caprioli A, Minelli F, Gianviti A, De Petris L, Edefonti A, Montini G, Ferretti A, De Palo T, Gaido M, Rizzoni G and Hemolytic Yremic Syndrome S, 2003. Shiga toxin-producing Escherichia coli infections associated with hemolytic uremic syndrome, Italy, 1988–2000. Emerging Infectious Diseases, 9, 106–108.
107. Tozzoli R, Caprioli A and Morabito S, 2005. Detection of toxB, a plasmid virulence gene of Escherichia coli O157, in enterohemorrhagic and enteropathogenic E. coli. Journal of Clinical Microbiology, 43, 4052–4056.
108. Underwood AP, Dallman T, Thomson NR, Williams M, Harker K, Perry N, Adak B, Willshaw G, Cheasty T, Green J, Dougan G, Parkhill J and Wain J, 2013. Public health value of next-generation DNA sequencing of Enterohemorrhagic Escherichia coli isolates from an outbreak. Journal of Clinical Microbiology, 51, 232–237.
109. USDA (U.S. Department of Agriculture), 2012. Risk Profile for Pathogenic Non-O157 Shiga Toxin-Producing Escherichia coli (non-O157 STEC). USDA Food Safety and Inspection Service, 64 pp.
110. Whittaker PJ, Sopwith W, Quigley C, Gillespie I, Willshaw GA, Lycett C, Surman-Lee S, Baxter D, Adak GK and Syed Q, 2009. A national outbreak of verotoxin-producing Escherichia coli O157 associated with consumption of lemon-and-coriander chicken wraps from a supermarket chain. Epidemiology and Infection, 137, 375–382.
111. Wickham ME, Lupp C, Mascarenhas M, Vazquez A, Coombes BK, Brown NF, Coburn BA, Deng WY, Puente JL, Karmali MA and Finlay BB, 2006. Bacterial genetic determinants of non-O157 STEC outbreaks and hemolytic-uremic syndrome after infection. Journal of Infectious Diseases, 194, 819–827.
112. Willshaw GA, Cheasty T, Smith HR, O'Brien SJ and Adak GK, 2001a. Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995–1998. Journal of medical microbiology, 50, 135–142.
113. Willshaw GA, Smith HR, Cheasty T and O'Brien SJ, 2001b. Use of strain typing to provide evidence for specific interventions in the transmission of VTEC O157 infections. International Journal of Food Microbiology, 66, 39–46.
114. Yang Z, Kovar J, Kim J, Nietfeldt J, Smith DR, Moxley RA, Olson ME, Fey PD and Benson AK, 2004. Identification of common subpopulations of non-sorbitol-fermenting, beta-glucuronidase-negative Escherichia coli O157:H7 from bovine production environments and human clinical samples. Applied and Environmental Microbiology, 70, 6846–6854.
115. Zhang WL, Mellmann A, Sonntag AK, Wieler L, Bielaszewska M, Tschape H, Karch H and Friedrich AW, 2007a. Structural and functional differences between disease-associated genes of enterohaemorrhagic Escherichia coli O111. International Journal of Medical Microbiology, 297, 17–26.
116. Zhang YX, Laing C, Steele M, Ziebell K, Johnson R, Benson AK, Taboada E and Gannon VPJ, 2007b. Genome evolution in major Escherichia coli O157:H7 lineages. Bmc Genomics, 8, 16.
117. Zweifel C, Schumacher S, Beutin L, Blanco J and Stephan R, 2006. Virulence profiles of Shiga toxin 2e-producing Escherichia coli isolated from healthy pig at slaughter. Veterinary Microbiology, 117, 328–332.