Development of a multiplex polymerase chain reaction assay for diarrheagenic Escherichia coli and Shigella spp. and its evaluation on colonies, culture broths, and stool

Diagnostic Microbiology and Infectious Disease

Volumn 73, Issue 2, 2012, Pages 121-128

  Taniuchi, Mami ^a   Walters, Carol C ^b   Gratz, Jean ^a,c   Maro, Athanasia ^c   Kumburu, Happiness ^c   Serichantalergs, Oralak ^d   Sethabutr, Orntipa ^d   Bodhidatta, Ladaporn ^d   Kibiki, Gibson ^c   Toney, Denise M ^b   Berkeley, Lynette ^e   Nataro, James P ^f   Houpt, Eric R ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

^b Virginia Department of General Services   (United States)

^c KILIMANJARO CHRISTIAN MEDICAL CENTRE   (Tanzania)

^d ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES   (Thailand)

^e UNIVERSITY OF MARYLAND   (United States)

^f UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Diarrhea; Diarrheagenic E. coli; Enteroaggregative E. coli; Enterohemorrhagic E. coli; Enteroinvasive E. coli; Enteropathogenic E. coli; Enterotoxigenic E. coli; Luminex; Multiplex PCR; PCR; Shiga toxin producing E. coli; Shigella; Virulence genes

Indexed keywords

DNA;

AAIC GENE; AATA GENE; AMPLICON; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM COLONY; BACTERIUM CULTURE; BACTERIUM DETECTION; BFPA GENE; DIARRHEAGENIC ESCHERICHIA COLI; DNA EXTRACTION; EAE GENE; FECES; FECES CULTURE; GENE IDENTIFICATION; IPAH GENE; LIMIT OF DETECTION; LT GENE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SHIGELLA; STX1 GENE; STX2 GENE; TANZANIA; VIRULENCE GENE;

COLONY COUNT, MICROBIAL; DIARRHEA; ENTEROBACTERIACEAE INFECTIONS; ESCHERICHIA COLI; FECES; HUMANS; LIMIT OF DETECTION; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; SHIGELLA; STATISTICS, NONPARAMETRIC;

EID: 84861185196     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.03.008     Document Type: Article

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