Alcohol dehydrogenases from Kluyveromyces marxianus: Heterologous expression in Escherichia coli and biochemical characterization

BMC Biotechnology

Volumn 14, Issue , 2014, Pages

  Liang, Jing juan ^a   Zhang, Mei ling ^a   Ding, Meng ^a   Mai, Zhi mao ^a   Wu, San xing ^a   Du, Yue ^a   Feng, Jia xun ^a  

^a GUANGXI UNIVERSITY   (China)

Author keywords

Alcohol dehydrogenase; Characterization; Expression; Gene cloning; Kluyveromyces marxianus

Indexed keywords

ACETALDEHYDE; CHARACTERIZATION; CLONING; ESCHERICHIA COLI; FERMENTATION; GENE ENCODING; YEAST;

ALCOHOL DEHYDROGENASE; BIOCHEMICAL CHARACTERISTICS; BIOCHEMICAL CHARACTERIZATION; ETHANOL FERMENTATION; EXPRESSION; GENE CLONING; HETEROLOGOUS EXPRESSION; KLUYVEROMYCES MARXIANUS;

ETHANOL;

2 PROPANOL; 5 HYDROXYMETHYLFURFURAL; ACETALDEHYDE; ALCOHOL; ALCOHOL DEHYDROGENASE; ALCOHOL DERIVATIVE; ANISALDEHYDE; BENZALDEHYDE; BUTANOL; CINNAMALDEHYDE; CROTONALDEHYDE; ENTEROPEPTIDASE; FUNGAL PROTEIN; FURFURAL; GLUTARALDEHYDE; GLYCERALDEHYDE; GLYCEROL; GLYOXAL; HYBRID PROTEIN; ISOBUTANOL; ISOPENTYL ALCOHOL; ISOVALERALDEHYDE; KMADH2 PROTEIN; KMADH3 PROTEIN; KMADH4 PROTEIN; METHANOL; PENTANOL; PROPANOL; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VANILLIN;

ALCOHOL PRODUCTION; AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA SEQUENCE; ELECTROPHORESIS; ENZYME ANALYSIS; ENZYME SPECIFICITY; ESCHERICHIA COLI; FERMENTATION; FUNGAL GENE; FUNGAL STRAIN; FUNGUS GROWTH; GENE EXPRESSION; HETEROLOGOUS EXPRESSION; HIGH TEMPERATURE; KLUYVEROMYCES MARXIANUS; KMADH GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PH; PROTEIN DEGRADATION; PROTEIN PURIFICATION; REDUCTION; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; ZYMOGRAPHY; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETICS; KINETICS; KLUYVEROMYCES; METABOLISM; MOLECULAR GENETICS; PHYLOGENY; SEQUENCE ALIGNMENT; TEMPERATURE;

ALCOHOL DEHYDROGENASE; AMINO ACID SEQUENCE; ESCHERICHIA COLI; ETHANOL; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; KINETICS; KLUYVEROMYCES; MOLECULAR SEQUENCE DATA; PHYLOGENY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84902548623     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-14-45     Document Type: Article

References (35)

1. Lane MM, Morrissey JP. Kluyveromyces marxianus: a yeast emerging from its sister's shadow. Fungal Biol Rev 2010, 24:17-26.
2. Schaffrath R, Breunig KD. Genetics and molecular physiology of the yeast Kluyveromyces lactis. Fungal Genet Biol 2000, 30:173-190. 10.1006/fgbi.2000.1221, 11035939.
3. Fonseca GG, Heinzle E, Wittmann C, Gombert AK. The yeast Kluyveromyces marxianus and its biotechnological potential. Appl Microbiol Biotechnol 2008, 79:339-354. 10.1007/s00253-008-1458-6, 18427804.
4. Nonklang S, Abdel-Banat BMA, Cha-aim K, Moonjai N, Hoshida H, Limtong S, Yamada M, Akada R. High-temperature ethanol fermentation and transformation with linear DNA in the thermotolerant yeast Kluyveromyces marxianus DMKU3-1042. Appl Environ Microbiol 2008, 74:7514-7521. 10.1128/AEM.01854-08, 2607150, 18931291.
5. Wilkins MR, Mueller M, Eichling S, Banat IM. Fermentation of xylose by the thermotolerant yeast strains Kluyveromyces marxianus IMB2, IMB4, and IMB5 under anaerobic conditions. Process Biochem 2008, 43:346-350.
6. Anderson PJ, McNeil K, Watson K. High-efficiency carbohydrate fermentation to ethanol at temperatures above 40°C by Kluyveromyces marxianus var. marxianus isolated from sugar mills. Appl Environ Microbiol 1986, 51:1314-1320. 239064, 16347089.
7. Banat IM, Nigam P, Marchant R. Isolation of thermotolerant, fermentative yeasts growing at 52°C and producing ethanol at 45°C and 50°C. World J Microbiol Biotechnol 1992, 8:259-263. 10.1007/BF01201874, 24425473.
8. Bai FW, Anderson WA, Moo-Young M. Ethanol fermentation technologies from sugar and starch feedstocks. Biotechnol Adv 2008, 26:89-105. 10.1016/j.biotechadv.2007.09.002, 17964107.
9. Ballesteros M, Oliva JM, Negro MJ, Manzanares P, Ballesteros I. Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SSF) with Kluyveromyces marxianus CECT 10875. Proc Biochem 2004, 39:1843-1848.
10. Tomás-Pejó E, García-Aparicio M, Negro MJ, Oliva JM, Ballesteros M. Effect of different cellulase dosages on cell viability and ethanol production by Kluyveromyces marxianus in SSF processes. Bioresour Technol 2009, 100:890-895. 10.1016/j.biortech.2008.07.012, 18722114.
11. de Smidt O, du Preez JC, Albertyn J. The alcohol dehydrogenases of Saccharomyces cerevisiae: a comprehensive review. FEMS Yeast Res 2008, 8:967-978. 10.1111/j.1567-1364.2008.00387.x, 18479436.
12. Lertwattanasakul N, Sootsuwan K, Limtong S, Thanonkeo P, Yamada M. Comparison of the gene expression patterns of alcohol dehydrogenase isozymes in the thermotolerant yeast Kluyveromyces marxianus and their physiological functions. Biosci Biotechnol Biochem 2007, 71:1170-1182. 10.1271/bbb.60622, 17485854.
13. Saliola M, Shuster JR, Falcone C. The alcohol dehydrogenase system in the yeast, Kluyveromyces lactis. Yeast 1990, 6:193-204. 10.1002/yea.320060304, 2190430.
14. Shain DH, Salvadore C, Denis CL. Evolution of the alcohol dehydrogenase (ADH) genes in yeast: characterization of a fourth ADH in Kluyveromyces lactis. Mol Gen Genet 1992, 232:479-488.
15. Jeong H, Lee DH, Kim SH, Kim HJ, Lee K, Song JY, Kim BK, Sung BH, Park JC, Sohn JH, Koo HM, Kim JF. Genome sequence of the thermotolerant yeast Kluyveromyces marxianus var. marxianus KCTC 17555. Eukaryot Cell 2012, 11:1584-1585. 10.1128/EC.00260-12, 3536290, 23193140.
16. Ladrière JM, Delcour J, Vandenhaute J. Sequence of a gene coding for a cytoplasmic alcohol dehydrogenase from Kluyveromyces marxianus ATCC 12424. Biochim Biophys Acta 1993, 1173:99-101. 10.1016/0167-4781(93)90252-9, 8485163.
17. Ladrière JM, Georis I, Guèrineau M, Vandenhaute J. Kluyveromyces marxianus exhibits an ancestral Saccharomyces cerevisiae genome organization downstream of ADH2. Gene 2000, 255:83-91. 10.1016/S0378-1119(00)00310-3, 10974568.
18. Lertwattanasakul N, Shigemoto E, Rodrussamee N, Limtong S, Thanonkeo P, Yamada M. The crucial role of alcohol dehydrogenase Adh3 in Kluyveromyces marxianus mitochondrial metabolism. Biosci Biotechnol Biochem 2009, 73:2720-2726. 10.1271/bbb.90609, 19966463.
19. Pang Z-W, Liang J-J, Qin X-J, Wang J-R, Feng J-X, Huang R-B. Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus. Biotechnol Lett 2010, 32:1847-1851. 10.1007/s10529-010-0384-8, 20803163.
20. Bennetzen JL, Hall BD. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase. J Biol Chem 1982, 257:3018-3025.
21. Suwannarangsee S, Oh DB, Seo JW, Kim CH, Rhee SK, Kang HA, Chulalaksananukul W, Kwon O. Characterization of alcohol dehydrogenase 1 of the thermotolerant methylotrophic yeast Hansenula polymorpha. Appl Microbiol Biotechnol 2010, 88:497-507. 10.1007/s00253-010-2752-7, 20635082.
22. Thomson JM, Gaucher EA, Burgan MF, De Kee DW, Li T, Aris JP, Benner SA. Resurrecting ancestral alcohol dehydrogenases from yeast. Nat Genet 2005, 37:630-635. 10.1038/ng1553, 3618678, 15864308.
23. Young ET, Sloan J, Miller B, Li N, van Riper K, Dombek KM. Evolution of a glucose-regulated ADH gene in the genus Saccharomyces. Gene 2000, 245:299-309. 10.1016/S0378-1119(00)00035-4, 10717481.
24. Jornvall H, Eklund H, Branden CI. Subunit conformation of yeast alcohol dehydrogenase. J Biol Chem 1978, 253:8414-8419.
25. Park YC, Yun NR, San KY, Bennett GN. Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene of Candida utilis ATCC 9950. J Ind Microbiol Biotechnol 2006, 33:1032-1036. 10.1007/s10295-006-0154-8, 16855819.
26. Bozzi A, Saliola M, Falcone C, Bossa F, Martini F. Structural and biochemical studies of alcohol dehydrogenase isozymes from Kluyveromyces lactis. Biochim Biophys Acta 1997, 1339:133-142. 10.1016/S0167-4838(96)00225-7, 9165108.
27. Brisdelli F, Saliola M, Pascarella S, Luzi C, Franceschini N, Falcone C, Martini F, Bozzi A. Kinetic properties of native and mutagenized isoforms of mitochondrial alcohol dehydrogenase III purified from Kluyveromyces lactis. Biochimie 2004, 86:705-712. 10.1016/j.biochi.2004.08.004, 15556281.
28. Ganzhorn AJ, Green DW, Hershey AD, Gould RM, Plapp BV. Kinetic characterization of yeast alcohol dehydrogenases. Amino acid residue 294 and substrate specificity. J Biol Chem 1987, 262:3754-3761.
29. Pal S, Park DH, Plapp BV. Activity of yeast alcohol dehydrogenases on benzyl alcohols and benzaldehydes: characterization of ADH1 from Saccharomyces carlsbergensis and transition state analysis. Chem Biol Interact 2009, 178:16-23. 10.1016/j.cbi.2008.10.037, 2712655, 19022233.
30. Lin Y, He P, Wang Q, Lu D, Li Z, Wu C, Jiang N. The alcohol dehydrogenase system in the xylose-fermenting yeast Candida maltosa. PLoS One 2010, 5:e11752. 10.1371/journal.pone.0011752, 2909261, 20668703.
31. Eklund H, Branden CI, Jornvall H. Structural comparisons of mammalian, yeast and bacillar alcohol dehydrogenases. J Mol Biol 1976, 102:61-73. 10.1016/0022-2836(76)90073-5, 775100.
32. Sambrook J, Russell DW. Molecular cloning: a laboratory mannual. 3rd ed 2001, Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
33. LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy JM. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Nat Biotechnol 1993, 11:187-193.
34. Cho JY, Jeffries TW. Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis. Appl Environ Microbiol 1999, 65:2363-2368. 91349, 10347014.
35. Lineweaver H, Burk D. The determination of enzyme dissociation constants. J Am Chem Soc 1934, 56:658-666.