A novel 2-step culture model for long-term in vitro maintenance of human pancreatic acinar cells

Pancreas

Volumn 43, Issue 5, 2014, Pages 762-767

  Bläuer, Merja ^a   Sand, Juhani ^a   Nordback, Isto ^a   Laukkarinen, Johanna ^a  

^a TAMPERE UNIVERSITY HOSPITAL   (Finland)

Author keywords

Acinar cells; Culture model; Exocrine cells; Human pancreas; In vitro

Indexed keywords

AMYLASE; CARBACHOL; CERULETIDE; CHOLECYSTOKININ RECEPTOR; MATRIGEL; COLLAGEN; DRUG COMBINATION; LAMININ; PROTEOGLYCAN;

ACINAR CELL; ADULT; AMYLASE RELEASE; ARTICLE; CELL STRUCTURE; CULTURE MEDIUM; CULTURE TECHNIQUE; DISSOCIATION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; IN VITRO STUDY; MONOLAYER CULTURE; PANCREAS; PRIMARY CULTURE; PRIORITY JOURNAL; BIOLOGICAL MODEL; CELL CULTURE; CYTOLOGY; DOSE RESPONSE; DRUG COMBINATION; DRUG EFFECTS; FEMALE; METABOLISM; MIDDLE AGED; PROCEDURES; REPRODUCIBILITY; SECRETION (PROCESS); TIME;

ACINAR CELLS; ADULT; AMYLASES; CARBACHOL; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CERULETIDE; COLLAGEN; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG COMBINATIONS; FEMALE; HUMANS; IN VITRO TECHNIQUES; LAMININ; MIDDLE AGED; MODELS, BIOLOGICAL; PANCREAS; PANCREAS, EXOCRINE; PROTEOGLYCANS; REPRODUCIBILITY OF RESULTS; TIME FACTORS;

EID: 84902506137     PISSN: 08853177     EISSN: 15364828     Source Type: Journal    
DOI: 10.1097/MPA.0000000000000105     Document Type: Article

References (34)

1. Chandra R, Liddle RA. Recent advances in pancreatic endocrine and exocrine secretion. Curr Opin Gastroenterol. 2011;27:439-443.
2. Weiss FU, Halangk W, Lerch MM. New advances in pancreatic cell physiology and pathophysiology. Best Pract Res Clin Gastroenterol. 2008;22:3-15.
3. Apte MV, Pirola RC, Wilson JS. Mechanisms of alcoholic pancreatitis. J Gastroenterol Hepatol. 2010;25:1816-1826.
4. Pandol SJ, Lugea A, Mareninova OA, et al. Investigating the pathobiology of alcoholic pancreatitis. Alcohol Clin Exp Res. 2011;35:830-837.
5. Sah RP, Saluja A. Molecular mechanisms of pancreatic injury. Curr Opin Gastroenterol. 2011;27:444-451.
6. Su KH, Cuthbertson C, Christophi C. Review of experimental animal models of acute pancreatitis. HPB (Oxford). 2006;8:264-286. (Pubitemid 44044243)
7. Case RM. Is the rat pancreas an appropriate model of the human pancreas? Pancreatology. 2006;6:180-190. (Pubitemid 44114486)
8. Parsa I, Marsh WH, Sutton AL, et al. Effects of dimethylnitrosamine on organ-cultured adult human pancreas. Am J Pathol. 1981;102:403-411. (Pubitemid 11143434)
9. Pandol SJ, Gukovsky I, Satoh A, et al. Animal and in vitro models of alcoholic pancreatitis: Role of cholecystokinin. Pancreas. 2003;27:297-300. (Pubitemid 37337893)
10. Thrower E, Husain S, Gorelick F. Molecular basis for pancreatitis. Curr Opin Gastroenterol. 2008;24:580-585.
11. Blinman TA, Gukovsky I, Mouria M, et al. Activation of pancreatic acinar cells on isolation from tissue: Cytokine upregulation via p38 MAP kinase. Am J Physiol Cell Physiol. 2000;279:1993-2003.
12. Lardon J, Bouwens L. Metaplasia in the pancreas. Differentiation. 2005;73:278-286. (Pubitemid 41583374)
13. Bläuer M, Nordback I, Sand J, et al. A novel explant outgrowth culture model for mouse pancreatic acinar cells with long-Term maintenance of secretory phenotype. Eur J Cell Biol. 2011;90:1052-1060.
14. Bläuer M, Tammela TL, Ylikomi T. A novel tissue-slice culture model for non-malignant human prostate. Cell Tissue Res. 2008;332:489-498.
15. Ji B, Bi Y, Simeone D, et al. Human pancreatic acinar cells do not respond to cholecystokinin. Pharmacol Toxicol. 2002;91:327-332. (Pubitemid 36132437)
16. Miyasaka K, Shinozaki H, Jimi A, et al. Amylase secretion from dispersed human pancreatic acini: Neither cholecystokinin a nor cholecystokinin B receptors mediate amylase secretion in vitro. Pancreas. 2002;35:161-165. (Pubitemid 34815913)
17. Susini C, Estival A, Scemama JL, et al. Studies on human pancreatic acini: Action of secretagogues on amylase release and cellular cyclic AMP accumulation. Pancreas. 1986;2:124-129. (Pubitemid 17019910)
18. Fanjul M, Gmyr V, Sengene's C, et al. Evidence for epithelial-mesenchymal transition in adult human pancreatic exocrine cells. J Histochem Cytochem. 2010;58:807-823.
19. Gmyr V, Kerr-Conte J, Belaich S, et al. Adult human cytokeratin 19-positive cells reexpress insulin promoter factor 1 in vitro: Further evidence for pluripotent pancreatic stem cells in humans. Diabetes. 2000;49:1671-1680.
20. Hall PA, Lemoine NR. Rapid acinar to ductal transdifferentiation in cultured human exocrine pancreas. J Pathol. 1992;166:97-103.
21. Lipsett MA, Castellarin ML, Rosenberg L. Acinar plasticity: Development of a novel in vitro model to study human acinar-To-duct-To-islet differentiation. Pancreas. 2007;34:452-457. (Pubitemid 46725787)
22. Rapoport DH, Schicktanz S, Gürleyik E, et al. Isolation and in vitro cultivation turns cells from exocrine human pancreas into multipotent stem-cells. Ann Anat. 2009;191:446-458.
23. Murphy JA, Criddle DN, Sherwood M, et al. Direct activation of cytosolic Ca2+ signaling and enzyme secretion by cholecystokinin in human pancreatic acinar cells. Gastroenterology. 2008;135:632-641.
24. Klein T, Heremans Y, Heimberg H, et al. Investigation and characterization of the duct cell-enriching process during serum-free suspension and monolayer culture using the human exocrine pancreas fraction. Pancreas. 2009;38:36-48.
25. Bläuer M, Heinonen PK, Martikainen PM, et al. A novel organotypic culture model for normal human endometrium: Regulation of epithelial cell proliferation by estradiol and medroxyprogesterone acetate. Hum Reprod. 2005;20:864-871. (Pubitemid 40667829)
26. Kim JB, Stein R, O'Hare MJ. Three-dimensional in vitro tissue culture models of breast cancerVa review. Breast Cancer Res Treat. 2004;85:281-291. (Pubitemid 38987263)
27. Peehl DM. Primary cell cultures as models of prostate cancer development. Endocr Relat Cancer. 2005;12:19-47. (Pubitemid 40477086)
28. Page H, Flood P, Reynaud EG. Three-dimensional tissue cultures: Current trends and beyond. Cell Tissue Res. 2013;352:123-131.
29. Ji B, Bi Y, Simeone D, et al. Human pancreatic acinar cells lack functional responses to cholecystokinin and gastrin. Gastroenterology. 2001;12:1380-1390. (Pubitemid 33140455)
30. Owyang C, Logsdon CD. New insights into neurohormonal regulation of pancreatic secretion. Gastroenterology. 2004;127:957-969. (Pubitemid 39208427)
31. Phillips PA, Yang L, Shulkes A, et al. Pancreatic stellate cells produce acetylcholine and may play a role in pancreatic exocrine secretion. Proc Natl Acad Sci U S A. 2010;40:17397-17402.
32. Gorelick FS, Thrower E. The acinar cell and early pancreatitis responses. Clin Gastroenterol Hepatol. 2009;7:S10YS14.
33. Perides G, Sharma A, Gopal A, et al. Secretin differentially sensitizes rat pancreatic acini to the effects of supramaximal stimulation with caerulein. Am J Physiol Gastrointest Liver Physiol. 2005;289:713-721.
34. Sharma A, Tao X, Gopal A, et al. Calcium dependence of proteinase-Activated receptor 2 and cholecystokinin-mediated amylase secretion from pancreatic acini. Am J Physiol Gastrointest Liver Physiol. 2005;289:686-695.