A tool for selective inline quantification of co-eluting proteins in chromatography using spectral analysis and partial least squares regression

Biotechnology and Bioengineering

Volumn 111, Issue 7, 2014, Pages 1365-1373

  Brestrich, Nina ^a   Briskot, Till ^a   Osberghaus, Anna ^a   Hubbuch, Jürgen ^a  

^a KARLSRUHE INSTITUTE OF TECHNOLOGY   (Germany)

Author keywords

Bioprocess monitoring; Chemometrics; Inline monitoring; Partial least squares regression; Process analytical technology; Protein analytics; Selective protein quantification

Indexed keywords

CHEMICAL DETECTION; CHROMATOGRAPHIC ANALYSIS; LEAST SQUARES APPROXIMATIONS; LIQUID CHROMATOGRAPHY; MATLAB; MIXTURES; SPECTRUM ANALYSIS;

BIOPROCESS MONITORING; CHEMOMETRICS; IN-LINE MONITORING; PARTIAL LEAST SQUARES REGRESSION; PROCESS ANALYTICAL TECHNOLOGY; PROTEIN QUANTIFICATION;

PROTEINS;

BUFFER; CO ELUTING PROTEIN; CYTOCHROME C; LYSOZYME; PROTEIN; RIBONUCLEASE A; SEPHAROSE; UNCLASSIFIED DRUG;

ARTICLE; BIOPROCESS; CALIBRATION; CHEMOMETRICS; CHROMATOGRAPHY; COMPUTER PROGRAM; FRACTIONATION; ION EXCHANGE CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; PARTIAL LEAST SQUARES REGRESSION; PROCESS MONITORING; PROTEIN PURIFICATION; REACTION TIME; SPECTROSCOPY; VALIDATION PROCESS; DEVICES; PROCEDURES; REGRESSION ANALYSIS;

CHROMATOGRAPHY; LEAST-SQUARES ANALYSIS; PROTEINS; SOFTWARE; SPECTRUM ANALYSIS;

EID: 84901596042     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25194     Document Type: Article

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