Monitoring the fractionation of a whey protein isolate during dead-end membrane filtration using fluorescence and chemometric methods

Biotechnology Progress

Volumn 26, Issue 1, 2010, Pages 168-178

  Elshereef, Rand ^a   Budman, Hector ^a   Moresoli, Christine ^a   Legge, Raymond L ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

Author keywords

LActalbumin; lactoglobulin; Bioprocess monitoring; Chemometric; Fluorescence spectroscopy; Membrane ultrafiltration

Indexed keywords

BIOPROCESS MONITORING; BOVINE SERUM ALBUMINS; CALIBRATION MODEL; CHEMOMETRIC METHOD; CHEMOMETRICES; FIBEROPTIC PROBES; IN-SITU MONITORING; INDIVIDUAL PROTEINS; INTRINSIC PROTEIN FLUORESCENCE; LACTOGLOBULIN; MEMBRANE FILTRATIONS; MEMBRANE ULTRAFILTRATION; MODEL SYSTEM; MULTICOMPONENTS; MULTIVARIATE CALIBRATION; MULTIVARIATE REGRESSION MODELS; MULTIVARIATE STATISTICAL TECHNIQUES; MULTIWAVELENGTH FLUORESCENCE; NEW APPROACHES; NON DESTRUCTIVE; PROTEIN CONCENTRATIONS; REASONABLE ACCURACY; REGRESSION MODEL; RETENTATE; WHEY PROTEIN ISOLATE;

BODY FLUIDS; CALIBRATION; CHROMATOGRAPHIC ANALYSIS; CONCENTRATION (PROCESS); FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; MEMBRANES; MICROFILTRATION; MULTIVARIANT ANALYSIS; REGRESSION ANALYSIS; SIZE EXCLUSION CHROMATOGRAPHY; ULTRAFILTRATION;

PROTEINS;

BOVINE SERUM ALBUMIN; LACTALBUMIN; LACTOGLOBULIN; MILK PROTEIN; WHEY PROTEIN;

ANIMAL; ARTICLE; ARTIFICIAL MEMBRANE; CATTLE; FIBER OPTICS; FLUORESCENCE; ISOLATION AND PURIFICATION; METHODOLOGY; MULTIVARIATE ANALYSIS; SPECTROFLUOROMETRY;

ANIMALS; CATTLE; FIBER OPTIC TECHNOLOGY; FLUORESCENCE; LACTALBUMIN; LACTOGLOBULINS; MEMBRANES, ARTIFICIAL; MILK PROTEINS; MULTIVARIATE ANALYSIS; SERUM ALBUMIN, BOVINE; SPECTROMETRY, FLUORESCENCE;

BOVINAE;

EID: 76849097265     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.293     Document Type: Article

References (24)

1. Huisman IH, Pradanos P, Hernandez A. The effect of protein-protein and protein-membrane interactions on membrane fouling in ultrafiltration. J Membr Sci. 2000;179:79-90.
2. Ghosh R. Protein Bioseparation Using Ultrafiltration: Theory, Applications and New Developments. London: Imperial College Press; 2003.
3. Wan H, Lu JR, Cui ZF. Separation of lysozyme from chicken egg white using ultrafiltration. Sep Purif Technol. 2006;48:133-142.
4. Deshpande SS. Principles and applications of luminescence spectroscopy. Crit Rev Food Sci Nutr. 2001;41:155-224
5. Baker A. Organic Substances and Sediments in Water, Vol. II: Processes and Analytical. Chelsea: Lewis Publishers; 1991.
6. Crespo JPSG, Trotin M, Hough D, Howell JA. Use of fluores-cence labeling to monitor protein fractionation by ultrafiltration under controlled permeate flux. J Membr Sci. 1999;155:209-230.
7. Guilbault GG. Practical Fluorescence. Theory, Methods and Techniques. New York: Marcel Dekker Inc.; 1973.
8. Lacowicz JR. Principles of Fluorescence Spectroscopy, 2nd ed. New York: Plenum; 1999.
9. Mycek MA, Pogue BW. Handbook of Biomedical Fluorescence. New York: Marcel Dekker; 2003.
10. Mayer LM, Schick LL, Loder TC. Dissolved protein fluore scence in two Maine estuaries. Mar Chem. 1999;64:171-179.
11. Elshereef R, Budman H, Moresoli C, Legge RL. Fluorescence-based soft-sensor for monitoring β-lactoglobulin and α-LActalbumin solubility during thermal aggregation. Biotechnol Bioeng. 2007;99:567-577.
12. Weinbreck F, Wientjes RHW, Nieuwenhuijse H, Robijn GW. Rheological properties of whey protein/gum arabic coacervates. J Rheol. 2004;48:1215-1228.
13. Elshereef R, Budman H, Moresoli C, Legge RL. Fluorescence spectroscopy as a tool for monitoring solubility and aggregation behavior of β-lactoglobulin after heat treatment. Biotechnol Bioeng. 2006;95:863-874.
14. Geladi P, Kowalski B. Partial least-squares regression: a tutorial. Anal Chim Acta. 1986;185:1-17.
15. Qin SJ, McAvoy TJ. Nonlinear PLS modeling using neural net-works. Comp Chem Eng. 1992;16:379-397.
16. Lemberge P, Van Espen PJ. Quantitative energy-dispersive X-ray fluorescence analysis of liquids using partial least-squares regression. X Ray Spectrom. 1999;28:77-85.
17. Ohno T, Bro R. Dissolved organic matter characterization using multi-way spectral decomposition of fluorescence landscapes. Soil Sci Soc Am J. 2006;70:2028-2037.
18. Ma CQ, Li KA, Zhao FL, Tong SY. A study on the reaction mechanism between chrome-azurol S and bovine serum albumin. Acta Chim Sinica. 1999;57:389-395.
19. Rao CM. Synchronous scan fluorescence spectroscopy of proteins and human eye lenses. Biochem Biophys Res Commun. 1991;176:1351-1357.
20. Christensen J, Norgaard L, Bro R, Engelsen SB. Multivariate autofluorescence of intact food systems. Chem Rev. 2006;106: 1979-1993.
21. Bhattacharjee S, Ghosh S, Datta S, Bhattacharjee C. Studies on ultra-filtration of casein whey using a rotating disk module: effects of pH and membrane disk rotation. Desalination. 2006;195:95-108.
22. Ghosh R, Cui ZF. Fractionation of BSA and lysozyme using ultrafiltration: effect of pH and membrane surface pretreatment. J Membr Sci. 1998;139:17-28.
23. Ghosh R, Cui ZF. Purification of lysozyme using ultrafiltration. Biotechnol Bioeng. 2000;68:191-202.
24. Ghosh R, Cui ZF. Analysis of protein transport and polarization through membrane using pulsed sample injection technique. J Membr Sci. 2000;175:75-84.