Extending the Schizosaccharomyces pombe molecular genetic toolbox

PLoS ONE

Volumn 9, Issue 5, 2014, Pages

  Fennessy, Dorota ^a   Grallert, Agnes ^a   Krapp, Andrea ^b   Cokoja, Adisa ^b,c   Bridge, Alan J ^a,d   Petersen, Janni ^a,e   Patel, Avinash ^a,f   Tallada, Victor A ^a,g   Boke, Elvan ^a,h   Hodgson, Ben ^a   Simanis, Viesturs ^b   Hagan, Iain M ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b EPFL   (Switzerland)

^c Swissmedic   (Switzerland)

^d SWISS INSTITUTE OF BIOINFORMATICS   (Switzerland)

^e UNIVERSITY OF MANCHESTER   (United Kingdom)

^f MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

^g UNIVERSIDAD PABLO DE OLAVIDE   (Spain)

^h HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYGROMYCIN B; KANAMYCIN; NOURSEOTHRICIN; ANTIINFECTIVE AGENT; STREPTOTHRICIN;

ANTIBIOTIC RESISTANCE; ARTICLE; AUXOTROPHY; BACTERIAL GENOME; BACTERIUM TRANSFORMATION; CHROMOSOME 3; CONTROLLED STUDY; DNA INTEGRATION; EXPRESSION VECTOR; GENE CASSETTE; GENE EXPRESSION; GENE FUNCTION; GENE LOCUS; GENE MUTATION; GENE SEQUENCE; GENE TARGETING; GENETIC SELECTION; GENETIC TRANSFORMATION; NONHUMAN; PLASMID; SCHIZOSACCHAROMYCES POMBE; SEQUENCE HOMOLOGY; DRUG EFFECTS; GENE VECTOR; GENETIC ENGINEERING; GENETICS; PROCEDURES; SCHIZOSACCHAROMYCES;

ANTI-BACTERIAL AGENTS; DRUG RESISTANCE, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; GENOME, BACTERIAL; SCHIZOSACCHAROMYCES; SEQUENCE HOMOLOGY, NUCLEIC ACID; STREPTOTHRICINS; TRANSFORMATION, GENETIC;

EID: 84901347614     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0097683     Document Type: Article

References (84)

1. Gutz H, Heslot H, Leupold U, Loprieno N (1974) Schizosaccharomyces pombe. In: King RC, editor. Handbook of Genetics. New York: Plenum press. 395-445.
2. Moreno S, Klar A, Nurse P (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods In Enzymology 194: 795-823.
3. Forsburg SL, Rhind N (2006) Basic methods for fission yeast. Yeast 23: 173-183.
4. Reaume SE, Tatum EL (1949) Spontaneous and nitrogen mustard-induced nutritional deficiencies in Saccharomyces cerevisiae. Arch Biochem 22: 331-338.
5. Leupold U (1970) Genetical methods for Schizosaccharomyces pombe. In: Prescott D, editor. Methods in cell physiology. New York: Academic Press. 169-177.
6. Carr AM, MacNeill SA, Hayles J, Nurse P (1989) Molecular cloning and sequence analysis of mutant alleles of the fission yeast cdc2 kinase gene: implications for cdc2 protein structure and function. Molecular and General Genetics, 218: 41-49. (Pubitemid 19176611)
7. Hottinger H, Pearson D, Yamao F, Gamulin V, Cooley L, et al. (1982) Nonsense suppression in Schizosaccharomyces pombe: the S. pombe Sup3-e tRNASer-UGA gene is active in S. cerevisiae. Mol Gen Genet 188: 219-224. (Pubitemid 13177169)
8. Hofer F, Hollerstein H, Janner F, Minet M, Thuriaux P, et al. (1979) The genetic fine structure of nonsense suppressors in Schizosaccharomyces pombe. Current Genetics 1: 45-61.
9. Beach D, Nurse P (1981) High-Frequency Transformation Of the Fission Yeast Schizosaccharomyces-Pombe. Nature 290: 140-142. (Pubitemid 11149687)
10. Russell P, Nurse P (1987) Negative regulation of mitosis by wee1 &, a gene encoding a protein- kinase homolog. Cell 49: 559-567.
11. Boeke JD, LaCroute F, Fink GR (1984) A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Molecular & general genetics : MGG 197: 345-346.
12. Grimm C, Kohli J, Murray J, Maundrell K (1988) Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4+ gene as a selectable marker. Molecular and General Genetics 215: 81-86. (Pubitemid 19021382)
13. Barbet N, Muriel WJ, Carr AM (1992) Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66.
14. Maundrell K (1993) Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 123: 127-130.
15. Maundrell K (1990) Nmt1 of Fission Yeast - a Highly Transcribed Gene Completely Repressed By Thiamine. Journal of Biological Chemistry 265: 10857-10864.
16. Craven RA, Griffiths DJ, Sheldrick KS, Randall RE, Hagan IM, et al. (1998) Vectors for the expression of tagged proteins in Schizosaccharomyces pombe. Gene 221: 59-68. (Pubitemid 28529609)
17. Kikuchi Y, Kitazawa Y, Shimatake H, Yamamoto M (1988) The primary structure of the leu1+ gene of Schizosaccharomyces pombe. Curr Genet 14: 375-379.
18. Apolinario E, Nocero M, Jin M, Hoffman CS (1993) Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr Genet 24: 491-495. (Pubitemid 24038918)
19. Burke JD, Gould KL (1994) Molecular cloning and characterization of the Schizosaccharomyces pombe his3 gene for use as a selectable marker. Mol Gen Genet 242: 169-176. (Pubitemid 24043217)
20. Fujita Y, Giga-Hama Y, Takegawa K (2005) Development of a genetic transformation system using new selectable markers for fission yeast Schizosaccharomyces pombe. Yeast 22: 193-202. (Pubitemid 40298149)
21. Hoffman RL, Hoffman CS (2006) Cloning the Schizosaccharomyces pombe lys2+ gene and construction of new molecular genetic tools. Curr Genet 49: 414-420. (Pubitemid 43733166)
22. Waddell S, Jenkins JR (1995) arg3+, a new selection marker system for Schizosaccharomyces pombe: application of ura4+ as a removable integration marker. Nucleic Acids Res 23: 1836-1837.
23. Ekwall K, Javerzat JP, Lorentz A, Schmidt H, Cranston G, et al. (1995) The chromodomain protein Swi6 - a key component at fission yeast centromeres. Science 269: 1429-1431.
24. Chino A, Watanabe K, Moriya H (2010) Plasmid construction using recombination activity in the fission yeast Schizosaccharomyces pombe. PloS one 5: e9652.
25. Keeney JB, Boeke JD (1994) Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856. (Pubitemid 24085636)
26. Matsuyama A, Arai R, Yashiroda Y, Shirai A, Kamata A, et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nature biotechnology 24: 841-847. (Pubitemid 44086626)
27. Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, et al. (2004) pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast 21: 1289-1305. (Pubitemid 39618520)
28. Hartmuth S, Petersen J (2009) Fission yeast Tor1 functions as part of TORC1 to control mitotic entry through the stress MAPK pathway following nutrient stress. J Cell Sci 122: 1737-1746.
29. Wach A, Brachat A, Pohlmann R, Philippsen P (1994) New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10: 1793-1808.
30. Longtine M, McKenzie A, Demarini DJ, Shah NG, Wach A, et al. (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953-961. (Pubitemid 28328001)
31. Goldstein AL, McCusker JH (1999) Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15: 1541-1553. (Pubitemid 29472165)
32. Wach A (1996) PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast 12: 259-265.
33. Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH (2002) A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Res 30: e23.
34. Brachmann CB, Davies A, Cost GJ, Caputo E, Li J, et al. (1998) Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132. (Pubitemid 28062863)
35. Bähler J, Steever AB, Wheatley S, Wang YL, Pringle JR, et al. (1998) Role of polo kinase and Mid1p in determining the site of cell division in fission yeast. Journal of Cell Biology 143: 1603-1616. (Pubitemid 29006506)
36. Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM (2005) Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. Yeast 22: 1013-1019. (Pubitemid 41582363)
37. Burland TG, Pallotta D, Tardif MC, Lemieux G, Dove WF (1991) Fission yeast promoter-probe vectors based on hygromycin resistance. Gene 100: 241-245.
38. Sato M, Dhut S, Toda T (2005) New drug-resistant cassettes for gene disruption and epitope tagging in Schizosaccharomyces pombe. Yeast 22: 583-591. (Pubitemid 40932159)
39. Knop M, Siegers K, Pereira G, Zachariae W, Winsor B, et al. (1999) Epitope tagging of yeast genes using a PCR-based strategy: More tags and improved practical routines. Yeast 15: 963-972.
40. Bähler J, Wu JQ, Longtine MS, Shah NG, McKenzie A, et al. (1998) Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14: 943-951. (Pubitemid 28328000)
41. Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, et al. (2004) A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21: 947-962. (Pubitemid 39206863)
42. Sheff MA, Thorn KS (2004) Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast 21: 661-670. (Pubitemid 38868184)
43. Germino M, Sohail H, Germino E, Germino J (2006) A vector for double epitope tagging with a recyclable marker. Yeast 23: 763-769. (Pubitemid 44275561)
44. Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, et al. (2006) HB tag modules for PCR-based gene tagging and tandem affinity purification in Saccharomyces cerevisiae. Yeast 23: 623-632. (Pubitemid 44150710)
45. Snaith HA, Anders A, Samejima I, Sawin KE (2010) New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods in cell biology 97: 147-172.
46. Erler A, Maresca M, Fu J, Stewart AF (2006) Recombineering reagents for improved inducible expression and selection marker re-use in Schizosaccharomyces pombe. Yeast 23: 813-823. (Pubitemid 44430582)
47. Grimm C, Kohli J (1988) Observations on integrative transformation in Schizosaccharomyces pombe. Molecular & general genetics : MGG 215: 87-93. (Pubitemid 19021383)
48. Grallert B, Nurse P, Patterson TE (1993) A study of integrative transformation in Schizosaccharomyces pombe. Molecular & general genetics : MGG 238: 26-32. (Pubitemid 23130629)
49. Critchlow SE, Jackson SP (1998) DNA end-joining: from yeast to man. Trends in biochemical sciences 23: 394-398. (Pubitemid 28511380)
50. Ninomiya Y, Suzuki K, Ishii C, Inoue H (2004) Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining. Proceedings of the National Academy of Sciences of the United States of America 101: 12248-12253. (Pubitemid 39145429)
51. Kooistra R, Hooykaas PJ, Steensma HY (2004) Efficient gene targeting in Kluyveromyces lactis. Yeast 21: 781-792. (Pubitemid 39076918)
52. Nayak T, Szewczyk E, Oakley CE, Osmani A, Ukil L, et al. (2006) A versatile and efficient gene-targeting system for Aspergillus nidulans. Genetics 172: 1557-1566.
53. Poggeler S, Kuck U (2006) Highly efficient generation of signal transduction knockout mutants using a fungal strain deficient in the mammalian ku70 ortholog. Gene 378: 1-10. (Pubitemid 44108460)
54. Villalba F, Collemare J, Landraud P, Lambou K, Brozek V, et al. (2008) Improved gene targeting in Magnaporthe grisea by inactivation of MgKU80 required for non-homologous end joining. Fungal genetics and biology : FG & B 45: 68-75. (Pubitemid 350110406)
55. Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, et al. (2012) Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology. PloS one 7: e39720.
56. Li ZH, Du CM, Zhong YH, Wang TH (2010) Development of a highly efficient gene targeting system allowing rapid genetic manipulations in Penicillium decumbens. Applied microbiology and biotechnology 87: 1065-1076.
57. Roguev A, Wiren M, Weissman JS, Krogan NJ (2007) High-throughput genetic interaction mapping in the fission yeast Schizosaccharomyces pombe. Nature methods 4: 861-866. (Pubitemid 350055586)
58. Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM (2005) Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. Yeast 15: 1013-1019. (Pubitemid 41582363)
59. Grallert A, Beuter C, Craven RA, Bagley S, Wilks D, et al. (2006) S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner. Genes Dev 20: 2421-2436. (Pubitemid 44320390)
60. Petersen J, Hagan IM (2005) Polo kinase links the stress pathway to cell cycle control and tip growth in fission yeast. Nature 435: 507-512. (Pubitemid 40734248)
61. Grallert A, Chan KY, Alonso-Nunez ML, Madrid M, Biswas A, et al. (2013) Removal of Centrosomal PP1 by NIMA Kinase Unlocks the MPF Feedback Loop to Promote Mitotic Commitment in S. pombe. Current biology : CB 23: 213-222.
62. Grallert A, Connolly Y, Smith DL, Simanis V, Hagan IM (2012) The S. pombe cytokinesis NDR kinase Sid2 activates Fin1 NIMA kinase to control mitotic commitment through Pom1/Wee1. Nature cell biology 14: 438-445.
63. Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N (2004) In vivo labeling of fission yeast DNA with thymidine and thymidine analogs. Methods 33: 213-219. (Pubitemid 38648842)
64. Egel R (1971) Physiological aspects of conjugation in fission yeast. Planta 98: 89-96.
65. Egel R, Willer M, Kjaerulff S, Davey J, Nielsen O (1994) Assessment of pheromone production and response in fission yeast by a halo test of induced sporulation. Yeast 10: 1347-1354. (Pubitemid 24323190)
66. Maekawa H, Usui T, Knop M, Schiebel E (2003) Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions. Embo J 22: 438-449. (Pubitemid 36193589)
67. Grallert A, Krapp A, Bagley S, Simanis V, Hagan IM (2004) Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity. Genes Dev 18: 1007-1021. (Pubitemid 38620241)
68. Basi G, Schmid E, Maundrell K (1993) TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene 123: 131-136. (Pubitemid 23035805)
69. Wilhelm BT, Marguerat S, Watt S, Schubert F, Wood V, et al. (2008) Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution. Nature 453: 1239-1243. (Pubitemid 351913597)
70. Forsberg SL (1993) Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Research 21: 2955-2956.
71. Kelly DA, Hoffman CS (2002) Gap repair transformation in fission yeast to exchange plasmid-selectable markers. BioTechniques 33: 978, 980, 982.
72. Miyoshi T, Sadaie M, Kanoh J, Ishikawa F (2003) Telomeric DNA ends are essential for the localization of Ku at telomeres in fission yeast. The Journal of biological chemistry 278: 1924-1931. (Pubitemid 36801433)
73. Kibe T, Tomita K, Matsuura A, Izawa D, Kodaira T, et al. (2003) Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer. Nucleic acids research 31: 5054-5063. (Pubitemid 37441873)
74. Petersen J, Hagan IM (2003) S. pombe aurora kinase/survivin is required for chromosome condensation and the spindle checkpoint attachment response. Curr Biol 13: 590-597. (Pubitemid 36391938)
75. Bitton DA, Grallert A, Scutt PJ, Yates T, Li Y, et al. (2011) Programmed fluctuations in sense/antisense transcript ratios drive sexual differentiation in S. pombe. Molecular systems biology 7: 559.
76. Fankhauser C, Simanis V (1994) The Cdc7 protein-kinase is a dosage-dependent regulator of septum formation in fission yeast. Embo Journal 13: 3011-3019. (Pubitemid 24213453)
77. Tanaka K, Petersen J, MacIver F, Mulvihill DP, Glover DM, et al. (2001) The role of Plo1 kinase in mitotic commitment and septation in Schizosaccharomyces pombe. EMBO Journal 20: 1259-1270. (Pubitemid 32233966)
78. Grallert A, Patel A, Tallada VA, Chan KY, Bagley S, et al. (2013) Centrosomal MPF triggers the mitotic and morphogenetic switches of fission yeast. Nature cell biology 15: 88-95.
79. Drummond DR, Hagan IM (1998) Mutations in the bimC box of Cut7 indicate divergence of regulation within the bimC family of kinesin related proteins. Journal of Cell Science 111: 853-865. (Pubitemid 28196069)
80. Tay YD, Patel A, Kaemena DF, Hagan IM (2013) Mutation of a conserved residue enhances the sensitivity of analogue-sensitised kinases to generate a novel approach to the study of mitosis in fission yeast. Journal of cell science 126: 5052-5061.
81. Murone M, Simanis V (1996) The fission yeast dma1 gene is a component of the spindle assembly checkpoint, required to prevent septum formation and premature exit from mitosis if spindle function is compromised. Embo Journal 15: 6605-6616. (Pubitemid 26413793)
82. Schmidt S, Sohrmann M, Hofmann K, Woolard A, Simanis V (1997) The Spg1 GTPase is an essential dosage-dependent inducer of septum formation in Schizosaccharomyces pombe. Genes and Development 11: 1519-1534. (Pubitemid 27274397)
83. Dischinger S, Krapp A, Xie L, Paulson JR, Simanis V (2008) Chemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network. J Cell Sci 121: 843-853. (Pubitemid 351578120)
84. Krapp A, Collin P, Cokoja A, Dischinger S, Cano E, et al. (2006) The Schizosaccharomyces pombe septation initiation network (SIN) is required for spore formation in meiosis. J Cell Sci 119: 2882-2891. (Pubitemid 44177866)