Chemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network

Journal of Cell Science

Volumn 121, Issue 6, 2008, Pages 843-853

  Dischinger, Sandra ^a,b   Krapp, Andrea ^a,b   Xie, Linfeng ^c   Paulson, James R ^c   Simanis, Viesturs ^a,b  

^a SWISS INST FOR EXP CANCER RESEARCH   (Switzerland)

^b EPFL   (Switzerland)

^c UNIVERSITY OF WISCONSIN OSHKOSH   (United States)

Author keywords

cdc2; Cell cycle; Chemical genetics; Cytokinesis; Yeast

Indexed keywords

1,3 BETA GLUCAN SYNTHASE; ADENOSINE TRIPHOSPHATE DERIVATIVE; CYCLIN DEPENDENT KINASE 1;

ARTICLE; CELL CYCLE ARREST; CELL CYCLE PHASE; CELL DIVISION; CELL MUTANT; CONTROLLED STUDY; CYTOKINESIS; DNA REPLICATION; GENE FREQUENCY; GENE INACTIVATION; GENE MUTATION; GENETIC ANALYSIS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; SCHIZOSACCHAROMYCES POMBE; SIGNAL TRANSDUCTION;

CDC2 PROTEIN KINASE; CELL CYCLE; CELL CYCLE PROTEINS; CYTOKINESIS; MEIOSIS; MITOSIS; MUTATION; PROTEIN KINASES; PROTEIN TYROSINE PHOSPHATASES; PYRAZOLES; PYRIMIDINES; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS;

SCHIZOSACCHAROMYCES POMBE; SCHIZOSACCHAROMYCETACEAE;

EID: 42549095588     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: 10.1242/jcs.021584     Document Type: Article

References (106)

1. Alfa, C. E., Booher, R., Beach, D. and Hyams, J. S. (1989). Fission yeast cyclin: subcellular localisation and cell cycle regulation. J. Cell Sci. Suppl. 12, 9-19.
2. Alin, C. E., Ducommun, B., Beach, D. and Hyams, J. S. (1990). Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast. Nature 347, 680-682.
3. Bahler, J., Steever, A. B., Wheatley, S., Wang, Y., Pringle, J. R., Gould, K. L. and McCollum, D. (1998a). Role of polo kinase and Mid1p in determining the site of cell division in fission yeast. J. Cell Biol. 143, 1603-1616.
4. Bahler, J., Wu, J. Q., Longtine, M. S., Shah, N. G., McKenzie, A., 3rd, Steever, A. B., Wach, A., Philippsen, P. and Pringle, J. R. (1998b). Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14, 943-951.
5. Balasubramanian, M. K., McCollum, D. and Gould, K. L. (1997). Cytokinesis in fission yeast Schizosaccharomyces pombe. Meth. Enzymol. 283, 494-506.
6. Bardin, A. J. and Amon, A. (2001). Men and sin: what's the difference? Nat. Rev. Mol. Cell Biol. 2, 815-826.
7. Beach, D, Durkacz, B. and Nurse, P. (1982). Functionally homologous cell cycle control genes in budding and fission yeast. Nature 300, 706-709.
8. Berthet, C. and Kaldis, P. (2007). Cell-specific responses to loss of cyclin-dependent kinases. Oncogene 26, 4469-4477.
9. Bishop, A. C., Kong, C.-Y., Shah, K., Witucki, L., Shokat, K. M. and Liu, Y. (1999). Generation of monospecific nanomolar tyrosine kinase inhibitors via a chemical genetic approach. J. Am. Chem. Soc. 121, 627-631.
10. Bishop, A. C. Ubersax, J. A., Petsch, D. T., Matheos, D. P., Gray, N. S., Blethrow, J., Shimizu, E., Tsien, J. Z., Schultz, P. G., Rose, M. D. et al. (2000). A chemical switch for inhibitor-sensitive alleles of any protein kinase. Nature 407, 395-401.
11. Booher, R. and Beach, D. (1986). Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe. Mol. Cell. Biol. 6, 3523-3530.
12. Booher, R. and Beach, D. (1988). Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules. EMBO J. 7, 2321-2327.
13. Broek, D., Bartlett, R., Crawford, K. and Nurse, P. (1991). Involvement of p34cdc2 in establishing the dependency of S phase on mitosis. Nature 349, 388-393.
14. Chang, L. and Gould, K. L. (2000). Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast. Proc. Natl. Acad. Sci. USA 97, 5249-5254.
15. Chang, L., Morrell, J. L., Feoktistova, A. and Gould, K. L. (2001). Study of cyclin proteolysis in anaphase-promoting complex (APC) mutant cells reveals the requirement for APC function in the final steps of the fission yeast septation initiation network. Mol. Cell. Biol. 21, 6681-6694.
16. Connolly, T. and Beach, D. (1994). Interaction between the Cig1 and Cig2 B-type cyclins in the fission yeast cell cycle. Mol. Cell. Biol. 14, 768-776.
17. Cortes, J. C., Konomi, M., Martins, I. M., Munoz, J., Moreno, M. B., Osumi, M., Duran, A. and Ribas, J. C. (2007). The (1,3)beta-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation. Mol. Microbiol. 65, 201-217.
18. Cueille, N. Salimova, E., Esteban, V., Blanco, M., Moreno, S., Bueno, A. and Simanis, V. (2001). Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis. J. Cell Sci. 114, 2649-2664.
19. Decottignies, A., Zarzov, P. and Nurse, P. (2001). In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis. J. Cell Sci. 114, 2627-2640.
20. Esteban, V., Blanco, M., Cueille, N., Simanis, V., Moreno, S. and Bueno, A. (2004). A role for the Cdc14-family phosphatase Flp1p at the end of the cell cycle in controlling the rapid degradation of the mitotic inducer Cdc25p in fission yeast. J. Cell Sci. 117, 2461-2468.
21. Fankhauser, C. and Simanis, V. (1993). The Schizosaccharomyces pombe cdc14 gene is required for septum formation and can also inhibit nuclear division. Mol. Biol. Cell 4, 531-539.
22. Fankhauser, C. and Simanis, V. (1994). The cdc7 protein kinase is a dosage dependent regulator of septum formation in fission yeast. EMBO J. 13, 3011-3019.
23. Fisher, D. and Nurse, P. (1995). Cyclins of the fission yeast Schizosaccharomyces pombe. Semin. Cell Biol. 6, 73-78.
24. Fisher, D. L. and Nurse, P. (1996). A single fission yeast mitotic cyclin B p34cdc2 kinase promotes both S-phase and mitosis in the absence of G1 cyclins. EMBO J. 15, 850-860.
25. Furge, K. A., Wong, K., Armstrong, J., Balasubramanian, M. and Albright, C. F. (1998). Byr4 and Cdc16 form a two-component GTPase-activating protein for the Spg1 GTPase that controls septation in fission yeast. Curr. Biol. 8, 947-954.
26. Ghiara, J. B., Richardson, H. E., Sugimoto, K., Henze, M., Lew, D. J. Wittenberg, C. and Read, S. I. (1991). A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis. Cell 65, 163-174.
27. Gould, K. (2004). Protein kinases driving the Cell Cycle. In The Molecular Biology of Schizosaccharomyces pombe (ed. R. Egel), pp. 27-40. Heidelberg: Springer-Verlag.
28. Gould, K. L. and Nurse, P. (1989). Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. Nature 342, 39-45.
29. Gould, K. L., Moreno, S. Owen, D. J. Sazer, S. and Nurse, P. (1991). Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function. EMBO J. 10, 3297-3309.
30. Grallert, A., Krapp, A., Bagley, S, Simanis, V. and Hagan, I. M. (2004). Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity. Genes Dev. 19, 1007-1021.
31. Guertin, D. A., Chang, L., Irshad, F., Gould, K. L. and McCollum, D. (2000). The role of the sid1p kinase and cdc14p in regulating the onset of cytokinesis in fission yeast. EMBO J. 19, 1803-1815.
32. Hagan, I. M. and Hyams, J. S. (1988). The use of cell division cycle mutants to investigate the control of microtubule distribution in the fission yeast Schizosaccharomyces pombe. J. Cell Sci. 89, 343-357.
33. Hagan, I., Hayles, J. and Nurse, P. (1988). Cloning and sequencing of the cyclin-related cdc13+ gene and a cytological study of its role in fission yeast mitosis. J. Cell Sci. 91, 587-595.
34. Hanefeld, U., Rees, C. W., White, A. J. P. and Williams, D. J. (1996). One-pot synthesis of tetrasubstituted pyrazoles-proof of regiochemistry. J. Chem. Soc. Perkin Trans. I 1996, 1545-1552.
35. Hayles, J., Aves, S. and Nurse, P. (1986). suc1 is an essential gene involved in both the cell cycle and growth in fission yeast. EMBO J. 5, 3373-3379.
36. He, X., Patterson, T. E. and Sazer, S. (1997). The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex. Proc. Natl. Acad. Sci. USA 94, 7965-7970.
37. Hermand, D., Pihlak, A., Westerling, T., Damagnez, V., Vandenhaute, J., Cottarel, G. and Makela, T. P. (1998). Fission yeast Csk1 is a CAK-activating kinase (CAKAK). EMBO J. 17, 7230-7238.
38. Hou, M. C., Salek, J. and McCollum, D. (2000). Mob1p interacts with the Sid2p kinase and is required for cytokinesis in fission yeast. Curr. Biol. 10, 619-622.
39. Hu, F., Wang, Y., Liu, D., Li, Y., Qin, J. and Elledge, S. J. (2001). Regulation of the Bub2/Bfa1 GAP complex by Cdc5 and cell cycle checkpoints. Cell 107, 655-665.
40. Jaspersen, S. L. and Morgan, D. O. (2000). Cdc14 activates cdc15 to promote mitotic exit in budding yeast. Curr. Biol. 10, 615-618.
41. Jiang, W. and Hallberg, R. (2000). Isolation and characterisation of par1+ and par2+: two Schizosaccharomyces pombe genes encoding B' subunits of protein phosphatase 2A. Genetics 154, 1025-1038.
42. Jiang, W. and Hallberg, R. L. (2001). Correct regulation of the septation initiation network in Schizosaccharomyces pombe requires the activities of par1 and par2. Genetics 158, 1413-1429.
43. Kaldis, P. and Aleem, E. (2005). Cell cycle sibling rivalry: Cdc2 vs. Cdk2. Cell Cycle 4, 1491-1494.
44. Kinoshita, N., Ohkura, H. and Yanagida, M. (1990). Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle. Cell 63, 405-415.
45. Krapp, A., Schmidt, S., Cano, E. and Simanis, V. (2001). S. pombe cdc11p, together with sid4p, provides an anchor for septation initiation network proteins on the spindle pole body. Curr. Biol. 11, 1559-1568.
46. Krapp, A., Gulli, M. P. and Simanis, V. (2004). SIN and the art of splitting the fission yeast cell. Curr. Biol. 14, R722-R730.
47. Kumada, K., Nakamura, T., Nagao, K., Funabiki, H., Nakagawa, T. and Yanagida, M. (1998). Cut1 is loaded onto the spindle by binding to Cut2 and promotes anaphase spindle movement upon Cut2 proteolysis. Curr. Biol. 8, 633-641.
48. Le Goff, X., Motegi, F., Salimova, E., Mabuchi, I. and Simanis, V. (2000). The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p. J. Cell Sci. 113, 4157-4163.
49. Le Goff, X., Buvelot, S., Salimova, E., Guerry, F., Schmidt, S., Cueille, N., Cano, E. and Simanis, V. (2001). The protein phosphatase 2A B'-regulatory subunit par1p is implicated in regulation of the S. pombe septation initiation network. FEBS Lett. 508, 136-142.
50. Lee, K. M., Saiz, J. E., Barton, W. A. and Fisher, R. P. (1999). Cdc2 activation in fission yeast depends on Mcs6 and Csk1, two partially redundant Cdk-activating kinases (CAKs). Curr. Biol. 9, 441-444.
51. Lee, M. G. and Nurse, P. (1987). Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature 327, 31-35.
52. Liu, J., Tang, X., Wang, H., Oliferenko, S. and Balasubramanian, M. K. (2002). The localization of the integral membrane protein Cps1p to the cell division site is dependent on the actomyosin ring and the septation-inducing network in Schizosaccharomyces pombe. Mol. Biol. Cell 13, 989-1000.
53. Liu, J., Wang, H., McCollum, D. and Balasubramanian, M. K. (1999). Drc1p/Cps1p, a 1,3-beta-glucan synthase subunit, is essential for division septum assembly in Schizosaccharomyces pombe. Genetics 153, 1193-1203.
54. Liu, Y., Bishop, A., Witucki, L., Kraybill, B., Shimizu, E., Tsien, J., Ubersax, J., Blethrow, J., Morgan, D. O. and Shokat, K. M. (1999). Structural basis for selective inhibition of Src family kinases by PP1. Chem. Biol. 6, 671-678.
55. MacNeill, S. A. and Nurse, P. (1993). Mutational analysis of the fission yeast p34cdc2 protein kinase gene. Mol. Gen. Genet. 236, 415-426.
56. MacNeill, S. A., Creanor, J. and Nurse, P. (1991). Isolation, characterisation and molecular cloning of new mutant alleles of the fission yeast p34cdc2+ protein kinase gene: identification of temperature-sensitive G2-arresting alleles. Mol. Gen. Genet. 229, 109-118.
57. Malumbres, M. and Barbacid, M. (2005). Mammalian cyclin-dependent kinases. Trends Biochem. Sci. 30, 630-641.
58. Marks, J., Hagan, I. M. and Hyams, J. S. (1986). Growth polarity and cytokinesis in fission yeast: the role of the cytoskeleton. J. Cell Sci. Suppl. 5, 229-241.
59. Martin-Castellanos, C. and Moreno, S. (1996). Regulation of G1 progression in fission yeast by the rum1+ gene product. Prog. Cell Cycle Res. 2, 29-35.
60. Martin-Castellanos, C., Blanco, M. A., de Prada, J. M. and Moreno, S. (2000). The puc1 cyclin regulates the G1 phase of the fission yeast cell cycle in response to cell size. Mol. Biol. Cell 11, 543-554.
61. Mishra, M., Karagiannis, J., Trautmann, S., Wang, H., McCollum, D. and Balasubramanian, M. K. (2004). The C1p1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe. J. Cell Sci. 117, 3897-3910.
62. Mondesert, O., McGowan, C. H. and Russell, P. (1996). Cig2, a B-type cyclin, promotes the onset of S in Schizosaccharomyces pombe. Mol. Cell. Biol. 16, 1527-1533.
63. Moreno, S., Hayles, J. and Nurse, P. (1989). Regulation of p34cdc2 protein kinase during mitosis. Cell 58, 361-372.
64. Moreno, S., Klar, A. and Nurse, P. (1991). Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Meth. Enzymol. 194, 795-823.
65. Morrell, J. L., Tomlin, G. C., Rajagopalan, S., Venkatram, S., Feoktistova, A. S., Tasto, J. J., Mehta, S., Jennings, J. L., Link, A., Balasubramanian, M. K. et al. (2004). Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB. Curr. Biol. 14, 579-584.
66. Mulvihill, D. P., Petersen, J., Ohkura, H., Glover, D. M. and Hagan, I. M. (1999). Plo1 kinase recruitment to the spindle pole body and its role in cell division in Schizosaccharomyces pombe. Mol. Biol. Cell 10, 2771-2785.
67. Munoz, M. J. and Jimenez, J. (1999). Genetic interactions between Hsp90 and the Cdc2 mitotic machinery in the fission yeast Schizosaccharomyces pombe. Mol. Gen. Genet. 261, 242-250.
68. Nakaseko, Y, Niwa, O. and Yanagida, M. (1984). A meiotic mutant of the fission yeast Schizosaccharomyces pombe that produces mature asci containing two diploid spores. J. Bacteriol. 157, 334-336.
69. Nasmyth, K. and Nurse, P. (1981). Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe. Mol. Gen. Genet. 182, 119-124.
70. Nurse, P. and Bissett, Y. (1981). Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast. Nature 292, 558-560.
71. Nurse, P., Thurlaux, P. and Nasmyth, K. (1976). Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol. Gen. Genet. 146, 167-178.
72. Ohkura, H. and Yanagida, M. (1991). S. pombe gene sds22+ essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1. Cell 64, 149-157.
73. Paulson, J. R. (2007). Inactivation of Cdk1/Cyclin B in metaphase-arrested mouse FT210 cells induces exit from mitosis without chromosome segregation or cytokinesis and allows passage through another cell cycle. Chromosoma 116, 215-225.
74. Pereira, G., Manson, C, Grindlay, J. and Schiebel, E. (2002). Regulation of the Bfa1p-Bub2p complex at spindle pole bodies by the cell cycle phosphatase Cdc14p. J. Cell Biol. 157, 367-379.
75. Potapova, T. A., Daum, J. R., Pittman, B. D., Hudson, J. R., Jones, T. N., Satinover, D. L., Stukenberg, P. T. and Gorbsky, G. J. (2006). The reversibility of mitotic exit in vertebrate cells. Nature 440, 954-958.
76. Rosenberg, J. A., Tomlin, G. C., McDonald, W. H., Snydsman, B. E., Muller, E. G., Yates, J. R., 3rd and Gould, K. L. (2006). Ppc89 links multiple proteins, including the septation initiation network, to the core of the fission yeast spindle-pole body. Mol. Biol. Cell 17, 3793-3805.
77. Russell, P. and Nurse, P. (1986). cdc25+ functions as an inducer in the mitotic control of fission yeast. Cell 45, 145-153.
78. Russell, P. and Nurse, P. (1987). Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog. Cell 49, 559-567.
79. Salimova, E., Sohrmann, M., Fournier, N. and Simanis, V. (2000). The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation. J. Cell Sci. 113, 1695-1704.
80. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning. New York: Cold Spring Harbor Laboratory Press.
81. Sazer, S. and Sherwood, S. W. (1990). Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast. J. Cell Sci. 97, 509-516.
82. Schmidt, S., Sohrmann, M., Hofmann, K., Woollard, A. and Simanis, V. (1997). The Spg1p GTPase is an essential, dosage-dependent inducer of septum formation in Schizosaccharomyces pombe. Genes Dev. 11, 1519-1534.
83. Seshan, A. and Amon, A. (2004). Linked for life: temporal and spatial coordination of late mitotic events. Curr. Opin. Cell Biol. 16, 41-48.
84. Simanis, V. (2003). Events at the end of mitosis in the budding and fission yeasts. J. Cell Sci. 116, 4263-4275.
85. Skoufias, D. A., Indorato, R. L., Lacroix, F., Panopoulos, A. and Margolis, R. L. (2007). Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed. J. Cell Biol. 179, 671-685.
86. Snaith, H. A. and Forsburg, S. L. (1999). Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genes. Genetics 152, 839-851.
87. Sohrmann, M., Schmidt, S., Hagan, I. and Simanis, V. (1998). Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase Cdc7p. Genes Dev. 12, 84-94.
88. Song, K., Mach, K. E., Chen, C. Y., Reynolds, T. and Albright, C. F. (1996). A novel suppressor of rasl in fission yeast, byr4, is a dosage- dependent inhibitor of cytokinesis. J. Cell Biol. 133, 1307-1319.
89. Sparks, C. A., Morphew, M. and McCollum, D. (1999). Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis. J. Cell Biol. 146, 777-790.
90. Stegmeier, F. and Amon, A. (2004). Closing mitosis: the functions of the Cdc14 phosphatase and its regulation. Annu. Rev. Genet. 38, 203-232.
91. Surana, U., Robitsch, H., Price, C., Schuster, T., Fitch, I., Futcher, A. B. and Nasmyth, K. (1991). The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae. Cell 65, 145-161.
92. Tanaka, K., Petersen, J., MacIver, F., Mulvihill, D. P., Glover, D. M. and Hagan, I. M. (2001). The role of Plol kinase in mitotic commitment and septation in Schizosaccharomyces pombe. EMBO J. 20, 1259-1270.
93. Tomlin, G. C., Morrell, J. L. and Gould, K. L. (2002). The spindle pole body protein cdc11p links sid4p to the fission yeast septation initiation network. Mol. Biol. Cell 13, 1203-1214.
94. Trautmann, S. and McCollum, D. (2002). Cell cycle: new functions for Cdc14 family phosphatases. Curr. Biol. 12, R733-R735.
95. Trautmann, S. and McCollum, D. (2005). Distinct nuclear and cytoplasmic functions of the S. pombe Cdc14-like phosphatase Clp1p/Flp1p and a role for nuclear shuttling in its regulation. Curr. Biol. 15, 1384-1389.
96. Trautmann, S., Wolfe, B. A., Jorgensen, P., Tyers, M., Gould, K. L. and McCollum, D. (2001). Fission yeast Clp1p phosphatase regulates G2/M transition and coordination of cytokinesis with cell cycle progression. Curr. Biol. 11, 931-940.
97. Trautmann, S., Rajagopalan, S. and McCollum, D. (2004). The S. pombe Cdc14-like phosphatase Clp1p regulates chromosome biorientation and interacts with Aurora kinase. Dev. Cell 7, 755-762.
98. Turnbull, E. L., Martin, I. V. and Fantes, P. A. (2006). Activity of Cdc2 and its interaction with the cyclin Cdc13 depend on the molecular chaperone Cdc37 in Schizosaccharomyces pombe. J. Cell Sci. 119, 292-302.
99. Umesono, K., Toda, T., Hayashi, S. and Yanagida, M. (1983). Cell division cycle genes nda2 and nda3 of the fission yeast Schizosaccharomyces pombe control microtubular organization and sensitivity to anti-mitotic benzimidazole compounds. J. Mol. Biol. 168, 271-294.
100. VerPlank, L. and Li, R. (2005). Cell cycle-regulated trafficking of Chs2 controls actomyosin ring stability during cytokinesis. Mol. Biol. Cell 16, 2529-2543.
101. Wolfe, B. A. and Gould, K. L. (2004). Fission yeast Clp1p phosphatase affects G2/M transition and mitotic exit through Cdc25p inactivation. EMBO J. 23, 919-929.
102. Wolfe, B. A. and Gould, K. L. (2005). Split decisions: coordinating cytokinesis in yeast. Trends Cell Biol. 15, 10-18.
103. Woods, A., Sherwin, T., Sasse, R., MacRae, T. H., Baines, A. J. and Gull, K. (1989). Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies. J. Cell Sci. 93, 491-500.
104. Wuarin, J., Buck, V., Nurse, P. and Millar, J. B. (2002). Stable association of mitotic cyclin B/Cdc2 to replication origins prevents endoreduplication. Cell 111, 419-431.
105. Yamano, H., Gannon, J. and Hunt, T. (1996). The role of proteolysis in cell cycle progression in Schizosaccharomyces pombe. EMBO J. 15, 5268-5279.
106. Yanagida, M., Yamashita, Y. M., Tatebe, H., Ishii, K., Kumada, K. and Nakaseko, Y. (1999). Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization. Philos. Trans. R. Soc. Lond. B Biol. Sci. 354, 1559-1569; discussion 1569-1570.