Production of Eicosapentaenoic Acid Using Heterotrophically Grown Microalgae

Single Cell Oils: Microbial and Algal Oils: Second Edition

Volumn , Issue , 2010, Pages 151-177

  Wen, Zhiyou ^a   Chen, Feng ^b  

^a Virginia Polytechnic Institute and State University   (United States)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84901206327     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-1-893997-73-8.50012-8     Document Type: Chapter

References (114)

1. Aasen I.M., Moretro T., Katla T., Axelsson L., Storro I. Influence of Complex Nutrients, Temperature and pH on Bacteriocin Production by lactobacillus sakei CCUG 42687. Appl. Microbiol. Biotechnol. 2000, 53:159-166.
2. Akimoto M., Shirai A., Ohtaguchi K., Koide K. Carbon Dioxide Fixation and Polyunsaturated Fatty Acid Production by the Red Alga Porphyridium Cruentum. Appl. Biochem. Biotechnol. 1998, 73:269-278.
3. Alonso D.L., del Castillo C.I.S., Grima E.M., Cohen Z. First Insights into Improvement of Eicosapentaenoic Acid Content in Phaeodactylum tricornutum (bacillariophyceae) by Induced Mutagenesis. J. Phycol. 1996, 32:339-345.
4. Apt K.E., Behrens P.W. Commercial developments in microalgal biotechnology. J. Phycol. 1999, 35:215-226.
5. Athalye S.K., Garcia R.A., Wen Z.Y. Use of Biodiesel-Derived Crude Glycerol for Producing Eicosapentaenoic Acid (EPA) by the Fungus pythium irregulare. J. Agri. Food Chem. 2009, 57:2744-2793.
6. Babcock T., Helton W.S., Espat N.J. Eicosapentaenoic Acid (EPA): An Anti-inflammatory Omega-3 Fat with Potential Clinical Applications. Nutrition 2000, 16:1116-1118.
7. Bajpai P.K., Bajpai P., Ward O.P. Optimization of Culture Conditions for Production of Eicosapentaenoic Acid by Mortierella-elongata NRRL-5513. J. Ind. Microbiol. 1992, 9:11-17.
8. Barclay W.R., Meager K.M., Abril J.R. Heterotrophic Production of Long-Chain Omega-3-Fatty-Acids Utilizing Algae and Algae-like Microorganisms. J. Appl. Phycol. 1994, 6:123-129.
9. Belarbi E.H., Molina E., Chisti Y. A Process for High Yield and Scaleable Recovery of High Purity Eicosapentaenoic Acid Esters from Microalgae and Fish Oil. Enzyme Micro. Technol. 2000, 26:516-529.
10. Blanchemain A., Grizeau D. Increased Production of Eicosapentaenoic Acid by Skeletonema Costatum Cells after Decantation at Low Temperature. Biotechnol. Tech. 1999, 13:497-501.
11. Blomquist G.J., Borgeson C.E., Vundla M. Polyunsaturated Fatty-Acids and Eicosanoids in Insects. Insect Biochem. 1991, 21:99-106.
12. Bonaa K.H., Bjerve K.S., Nordoy A. Habitual Fish Consumption, Plasma Phospholipid Fatty-Acids, and Serum-Lipids-The Tromso Study. Am. J. Clinical Nutri. 1992, 55:1126-1134.
13. Bowman J.P., McCammon S.A., Lewis T., Skerratt J.H., Brown J.L., Nichols D.S., McMeekin T.A. Psychroflexus Torquis Gen. Nov., sp. Nov., a Psychrophilic Species from Antarctic Sea Ice, and Reclassification of Flavobacterium Gondwanense (Dobson et al., 1993) as Psychroflexus Gondwanense Gen. Nov., Comb. Nov. Microbiology UK 1998, 144:1601-1609.
14. Braden L.M., Carroll K.K. Dietary Polyunsaturated Fat in Relation to Mammary Carcinogenesis in Rats. Lipids 1986, 21:285-288.
15. Calder P.C. N-3 Polyunsaturated Fatty Acids and Cytokine Production in Health and Disease. Annals Nutri. Metabol. 1997, 41:203-234.
16. Ceron Garcia M.C., Camacho F.G., Miron A.S., Sevilla J.M.F., Chisti Y., Grima E.M. Mixotrophic Production of Marine Microalga Phaeodactylum tricornutum on Various Carbon Sources. J. Microbiol. Biotechnol. 2006, 16:689-694.
17. Chen F. High Cell Density Culture of Microalgae in Heterotrophic Growth. Trends Biotechnol. 1996, 14:421-426.
18. Chen F., Johns M.R. Effect of C/N Ratio and Aeration on the Fatty-Acid Composition of Heterotrophic Chlorella-Sorokiniana. J. Appl. Phycol. 1991, 3:203-209.
19. Chen F., Johns M.R. A Strategy for High Cell-Density Culture of Heterotrophic Microalgae with Inhibitory Substrates. J. Appl. Phycol. 1995, 7:43-46.
20. Chen G.Q., Jiang Y., Chen F. Fatty Acid and Lipid Class Composition of the Eicosapentaenoic Acid-Producing Microalga, Nitzschia laevis. Food Chem. 2007, 104:1580-1585.
21. Chen G.Q., Jiang Y., Chen F. Salt-Induced Alterations in Lipid Composition of Diatom Nitzschia laevis (bacillariophyceae) under Heterotrophic Culture Condition. J. Phycol. 2008, 44:1309-1314.
22. Chen G.Q., Jiang Y., Chen F. Variation of Lipid Class Composition in Nitzschia laevis as a Response to Growth Temperature Change. Food Chem. 2008, 109:88-94.
23. Cheng M.H., Walker T.H., Hulbert G.J., Raman D.R. Fungal Production of Eicosapentaenoic and Arachidonic Acids from Industrial Waste Streams and Crude Soybean Oil. Biores. Technol. 1999, 67:101-110.
24. Chiou S.Y., Su W.W., Su Y.C. Optimizing Production of Polyunsaturated Fatty Acids in Marchantia polymorpha Cell Suspension Culture. J. Biotechnol. 2001, 85:247-257.
25. Cohen Z. The Production Potential of Eicosapentaenoic and Arachidonic Acids by the Red Alga Porphyridium cruentum. J. Am. Oil Chem. Soc. 1990, 67:916-920.
26. Cohen Z. Production Potential of Eicosapentaenoic Acid by Monodus subterraneus. J. Am. Oil Chem. Soc. 1994, 71:941-945.
27. Cohen Z., Didi S., Heimer Y.M. Overproduction of Gamma-Linolenic and Eicosapentaenoic Acids by Algae. Plant Physiol. 1992, 98:569-572.
28. Cohen Z., Heimer Y.M. Production of Polyunsaturated Fatty Acids (EPA, ARA, and GLA) by the Microalgae Porphyridium and Spirulina. Industrial Applications of Single Cell Oils 1992, 243-273. AOCS Press, Champaign, IL. D.J. Kyle, C. Ratledge (Eds.).
29. Committee Paper for Discussion. Microalga (odontella aurita): A Notification under Article 5 of the Novel Food Regulation (ec) 258/97 (accessed Jul 2009). http://www.foodstandards.gov.uk/multimedia/pdfs/acnfp593.pdf.
30. Coombs J., Halicki P.J., Holm-Hansen O., Volcani B.E. Studies on the Biochemistry and Fine Structure of Silicate Shell Formation in Diatoms. II. Changes in Concentration of Nucleoside Triphosphates in Silicon-Starvation Synchrony of Navicula pelliculosa (breb.) hilse. Exp. Cell Res. 1967, 47:315-328.
31. de Swaaf M.E., Pronk J.T., Sijtsma L. Fed-Batch Cultivation of the Docosahexaenoic-Acid-Producing Marine Alga Crypthecodinium cohnii on Ethanol. Appl. Microbiol. Biotechnol. 2003, 61:40-43.
32. de Swaaf M.E., Sijtsma L., Pronk J.T. High-Cell-Density Fed-Batch Cultivation of the Docosahexaenoic Acid-Producing Marine Alga Crypthecodinium cohnii. Biotechnol. Bioeng. 2003, 81:666-672.
33. Emsley R., Oosthuizen P., van Rensburg S.J. Clinical Potential of Omega-3 Fatty Acids in the Treatment of Schizophrenia. CNS Drugs 2003, 17:1081-1091.
34. Enright C.T., Newkirk G.F., Craigie J.S., Castell J.D. Growth of Juvenile Ostrea-Edulis l Fed Chaetoceros gracilis Schutt of Varied Chemical-Composition. J. Exp. Marine Bio. Eco. 1986, 96:15-26.
35. Eppley R.W. The Growth and Culture of Diatom. The Biology of Diatoms 1977, 24-64. Blackwell Scientific, Oxford. D. Werner (Ed.).
36. Fernandez F.G.A., Sevilla J.M.F., Perez J.A.S., Grima E.M., Chisti Y. Airlift-Driven External-Loop Tubular Photobioreactors for Outdoor Production of Microalgae: Assessment of Design and Performance. Chem. Eng. Sci. 2001, 56:2721-2732.
37. Garcia M.C.C., Sevilla J.M.F., Fernandez F.G.A., Grima E.M., Camacho F.G. Mixotrophic Growth of Phaeodactylum tricornutum on Glycerol: Growth Rate and Fatty Acid Profile. J. Appl. Phycol. 2000, 12:239-248.
38. Garcia M.C.C., Miron A.S., Sevilla J.M.F., Grima E.M., Camacho F.G. Mixotrophic Growth of the Microalga Phaeodactylum tricornutum-Influence of Different Nitrogen and Organic Carbon Sources on Productivity and Biomass Composition. Process Biochem. 2005, 40:297-305.
39. Gentile G., Bonasera V., Amico C., Giuliano L., Yakimov M.M. Shewanella sp ga-22, a Psychrophilic Hydrocarbonoclastic Antarctic Bacterium Producing Polyunsaturated Fatty Acids. J. Appl. Microbiol. 2003, 95:1124-1133.
40. Gill I., Valivety R. Polyunsaturated Fatty Acids. 1. Occurrence, Biological Activities and Applications. Trends Biotechnol. 1997, 15:401-409.
41. 2 and Nitrogen Supply on Lipid Class Composition of Dunaliella viridis. J. Appl. Phycol. 1998, 10:135-144.
42. Grima E.M., Perez J.A.S., Sanchez J.L.G., Camacho F.G., Alonso D.L. EPA from Isochrysis galbana-Growth-Conditions and Productivity. Process Biochem. 1992, 27:299-305.
43. Grima E.M., Perez J.A.S., Camacho F.G., Sanchez J.L.G., Alonso D.L. N-3 PUFA Productivity in Chemostat Cultures of Microalgae. Appl. Microbiol. Biotechnol. 1993, 38:599-605.
44. Grima E.M., Perez J.A.S., Camacho F.G., Sevilla J.M.F., Fernandez F.G.A. Effect of Growth-Rate on the Eicosapentaenoic Acid and Docosahexaenoic Acid Content of Isochrysis galbana in Chemostat Culture. Appl. Microbiol. Biotechnol. 1994, 41:23-27.
45. Grima E.M., Fernandez F.G.A., Camacho F.G., Chisti Y. Photobioreactors: Light Regime, Mass Transfer, and Scaleup. J. Biotechnol. 1999, 70:231-247.
46. Grima E.M., Belarbi E.H., Fernandez F.G.A., Medina A.R., Chisti Y. Recovery of Microalgal Biomass and Metabolites: Process Options and Economics. Biotechnol. Adv. 2003, 20:491-515.
47. Gurr M.I. Biosynthesis of Fats. The Role of Fats in Human Nutrition 1985, 24-28. Ellis Horwood Ltd., Chichester. F.B. Padley, J. Podmore (Eds.).
48. Gurr M.I. The Nutritional and Biological Properties of the Polyunsaturated Fatty Acids. Lipids in Nutrition and Health: A Reappraisal 1999, 119-160. The Oily Press, Bridgewater. M.I. Gurr (Ed.).
49. Hamamoto T., Takata N., Kudo T., Horikoshi K. Effect of Temperature and Growth-Phase on Fatty-Acid Composition of the Psychrophilic Vibrio sp Strain No-5710. FEMS Microbiol. Lett. 1994, 119:77-81.
50. Hostmark A.T., Bjerkedal T., Kierulf P., Flaten H., Ulshagen K. Fish Oil and Plasma-Fibrinogen. Bri. Med. J. 1988, 297:180-181.
51. Hu Q., Hu Z.Y., Cohen Z., Richmond A. Enhancement of Eicosapentaenoic Acid (EPA) and Gamma-Linolenic Acid (GLA) Production by Manipulating Algal Density of Outdoor Cultures of Monodus subterraneus (Eustigmatophyta) and Spirulina platensis (cyanobacteria). Eur. J. Phycol. 1997, 32:81-86.
52. Jareonkitmongkol S., Shimizu S., Yamada H. Production of an Eicosapentaenoic Acid-Containing Oil by a Delta-12 Desaturase-Defective Mutant of Mortierella alpina 1S-4. J. Am. Oil Chem. Soc. 1993, 70:119-123.
53. Kato C., Nogi Y. Correlation between Phylogenetic Structure and Function: Examples from Deep-Sea Shewanella. FEMS Microbiol. Eco. 2001, 35:223-230.
54. Kitano M., Matsukawa R., Karube I. Changes in Eicosapentaenoic Acid Content of Navicula saprophila, Rhodomonas salina and Nitzschia sp under Mixotrophic Conditions. J. Appl. Phycol. 1997, 9:559-563.
55. Lebeau T., Robert J.M. Diatom Cultivation and Biotechnologically Relevant Products. Part I: Cultivation at Various Scales. Appl. Microbiol. Biotechnol. 2003, 60:612-623.
56. Lebeau T., Robert J.M. Diatom Cultivation and Biotechnologically Relevant Products. Part II: Current and Putative Products. Appl. Microbiol. Biotechnol. 2003, 60:624-632.
57. Martin-Jezequel V., Hildebrand M., Brzezinski M.A. Silicon Metabolism in Diatoms: Implications for Growth. J. Phycol. 2000, 36:821-840.
58. Miron A.S., Gomez A.C., Camacho F.G., Grima E.M., Chisti Y. Comparative Evaluation of Compact Photobioreactors for Large-Scale Monoculture of Microalgae. J. Biotechnol. 1999, 70:249-270.
59. Miron A.S., Garcia M.C.C., Camacho F.G., Grima E.M., Chisti Y. Growth and Biochemical Characterization of Microalgal Biomass Produced in Bubble Column and Airlift Photobioreactors: Studies in Fed-Batch Culture. Enzyme Micro. Technol. 2002, 31:1015-1023.
60. Molina E., Fernandez J., Acien F.G., Chisti Y. Tubular Photobioreactor Design for Algal Cultures. J. Biotechnol. 2001, 92:113-131.
61. Nettleton J.A. Omega-3 Fatty Acids and Health 1995, Chapman & Hall, New York.
62. Ohta S., Chang T., Aozasa O., Ikegami N., Miyata H. Alterations in Fatty-Acid Composition of Marine Red Alga Porphyridium purpureum by Environmental-Factors. Botanica Marina 1993, 36:103-107.
63. Otero A., Garcia D., Fabregas J. Factors Controlling Eicosapentaenoic Acid Production in Semicontinuous Cultures of Marine Microalgae. J. Appl. Phycol. 1997, 9:465-469.
64. Otero A., Garcia D., Morales E.D., Aran J., Fabregas J. Manipulation of the Biochemical Composition of the Eicosapentaenoic Acid-Rich Microalga Isochrysis galbana in Semicontinuous Cultures. Biotechnol. Appl. Biochem. 1997, 26:171-177.
65. Peet M. Nutrition and Schizophrenia: Beyond Omega-3 Fatty Acids. Prostaglandins Leukotrienes Essen. Fatty Acids 2004, 70:417-422.
66. Ponizovsky A.M., Barshtein G., Bergelson L.D. Biochemical Alterations of Erythrocytes as an Indicator of Mental Disorders: An Overview. Harvard Review Psychiatry 2003, 11:317-332.
67. Ratledge C. Biotechnology of Oils and Fats. Microbial Lipids 1989, Vol. 2:567-668. Academic Press, London. C. Ratledge, S.G. Wilkinson (Eds.).
68. Ratledge C. Single-Cell Oils-Have They a Biotechnological Future. Trends Biotechnol. 1993, 11:278-284.
69. Ratledge C. Microbial Lipid. Biotechnology: Products of Second Metabolism 1997, Vol. 7:133-197. VCH Press, Weinheim. 2nd edn. H. Kleinkauf, H. Von Dohre (Eds.).
70. Renaud S.M., Parry D.L., Thinh L.V. Microalgae for Use in Tropical Aquaculture .1. Gross Chemical and Fatty-Acid Composition of 12 Species of Microalgae from the Northern-Territory, Australia. J. Appl. Phycol. 1994, 6:337-345.
71. Renaud S.M., Thinh L.V., Parry D.L. The Gross Chemical Composition and Fatty Acid Composition of 18 Species of Tropical Australian Microalgae for Possible Use in Mariculture. Aquaculture 1999, 170:147-159.
72. Ringo E., Sinclair P.D., Birkbeck H., Barbour A. Production of Eicosapentaenoic Acid (20-5 n-3) by Vibrio pelagius Isolated from Turbot (Scophthalmus maximus) Larvae. Appl. Environ. Microbiol. 1992, 58:3777-3778.
73. Satomi M., Oikawa H., Yano Y. Shewanella marinintestina sp nov., Shewanella schlegeliana sp nov and Shewanella sairae sp nov., Novel Eicosapentaenoic-Acid-Producing Marine Bacteria Isolated from Sea-Animal Intestines. Inter. J. Sys. Evol. Microbiol. 2003, 53:491-499.
74. Seto A., Wang H.L., Hesseltine C.W. Culture Conditions Affect Eicosapentaenoic Acid Content of Chlorella minutissima. J. Am. Oil Chem. Soc. 1984, 61:892-894.
75. Sevilla J.M.F., Grima E.M., Camacho F.G., Fernandez F.G.A., Perez J.A.S. Photolimitation and Photoinhibition as Factors Determining Optimal Dilution Rate to Produce Eicosapentaenoic Acid from Cultures of the Microalga Isochrysis galbana. Appl. Microbiol. Biotechnol. 1998, 50:199-205.
76. Shimizu Y. Microalgal Metabolites: A New Perspective. Ann. Rev. Microbiol. 1996, 50:431-465.
77. Shimizu S., Kawashima H., Shinmen Y., Akimoto K., Yamada H. Production of Eicosapentaenoic Acid by Mortierella Fungi. J. Am. Oil Chem. Soc. 1988, 65:1455-1459.
78. Shimizu S., Shinmen Y., Kawashima H., Akimoto K., Yamada H. Fungal Mycelia as a Novel Source of Eicosapentaenoic Acid-Activation of Enzyme(s) Involved in Eicosapentaenoic Acid Production at Low-Temperature. Biochem. Biophy. Res. Com. 1988, 150:335-341.
79. Shimizu S., Kawashima H., Akimoto K., Shinmen Y., Yamada H. Microbial Conversion of an Oil Containing Alpha-Linolenic Acid to an Oil Containing Eicosapentaenoic Acid. J. Am. Oil Chem. Soc. 1989, 66:342-347.
80. Shirasaka N., Shimizu S. Production of Eicosapentaenoic Acid by Saprolegnia sp 28ytf-1. J. Am. Oil Chem. Soc. 1995, 72:1545-1549.
81. Singh A., Ward O.P. Microbial Production of Docosahexaenoic Acid (DHA, C22:6). Advances in Appl. Microbiol. 1997, 45:21-312.
82. Springer M., Franke H., Pulz O. Increase of the content of Polyunsaturated Fatty-Acids in Porphyridium-cruentum by Low-Temperature Stress and Acetate Supply. J. Plant Physiol. 1994, 143:534-537.
83. Srivastava V.C., Manderson G.J., Bhamidimarri R. Inhibitory Metabolites Production by the Cyanobacterium Fischerella muscicola. Microbiol. Res. 1999, 153:309-317.
84. Stinson E.E., Kwoczak R., Kurantz M.J. Effect of cultural conditions on production of eicosapentaenoic acid by Pythium irregulare. J. Ind. Microbiol. 1991, 8:171-178.
85. Stredansky M., Conti E., Salaris A. Production of Polyunsaturated Fatty Acids by Pythium ultimum in Solid-State Cultivation. Enzyme Micro. Technol. 2000, 26:304-307.
86. Su K.P., Huang S.Y., Chiu C.C., Shen W.W. Omega-3 Fatty Acids in Major Depressive Disorder-A Preliminary Double-blind, Placebo-controlled Trial. Eur. Neuropsychopharmacol. 2003, 13:267-271.
87. Sukenik A. Ecophysiological Considerations in the Optimization of Eicosapentaenoic Acid Production by Nannochloropsis sp (Eustigmatophyceae). Biores. Technol. 1991, 35:263-269.
88. Taguchi S., Hirata J.A., Laws E.A. Silicate Deficiency and Lipid-Synthesis of Marine Diatoms. J. Phycol. 1987, 23:260-267.
89. Takeyama H., Takeda D., Yazawa K., Yamada A., Matsunaga T. Expression of the Eicosapentaenoic Acid Synthesis Gene Cluster from Shewanella sp. in a Transgenic Marine Cyanobacterium, Synechococcus sp. Microbiol. UK 1997, 143:2725-2731.
90. Tan C.K., Johns M.R. Screening of Diatoms for Heterotrophic Eicosapentaenoic Acid Production. J. Appl. Phycol. 1996, 8:59-64.
91. Tredici M.R. Bioreactors, Photo. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation 1999, Vol. 1:395-419. Wiley, New York, . M.C. Flickinger, S.W. Drew (Eds.).
92. Vazhappilly R., Chen F. Eicosapentaenoic Acid and Docosahexaenoic Acid Production Potential of Microalgae and Their Heterotrophic Growth. J. Am. Oil Chem. Soc. 1998, 75:393-397.
93. Vazhappilly R., Chen F. Heterotrophic Production Potential of Omega-3 Polyunsaturated Fatty Acids by Microalgae and Algae-like Microorganisms. Botanica Marina 1998, 41:553-558.
94. Wen Z.Y., Chen F. Heterotrophic Production of Eicosapentaenoid Acid by the Diatom Nitzschia laevis: Effects of Silicate and Glucose. J. Ind. Microbiol. & Biotechnol. 2000, 25:218-224.
95. Wen Z.Y., Chen F. Production Potential of Eicosapentaenoic Acid by the Diatom Nitzschia laevis. Biotechnol. Lett. 2000, 22:727-733.
96. Wen Z.Y., Chen F. Application of Statistically-Based Experimental Designs for the Optimization of Eicosapentaenoic Acid Production by the Diatom Nitzschia laevis. Biotechnol. Bioeng 2001, 75:159-169.
97. Wen Z.Y., Chen F. Optimization of Nitrogen Sources for Heterotrophic Production of Eicosapentaenoic Acid by the Diatom Nitzschia laevis. Enzyme Micr. Technol. 2001, 29:341-347.
98. Wen Z.Y., Chen F. A Perfusion-Cell Bleeding Culture Strategy for Enhancing the Productivity of Eicosapentaenoic Acid by Nitzschia laevis. Appl. Microbiol. Biotechnol. 2001, 57:316-322.
99. Wen Z.Y., Chen F. Continuous Cultivation of the Diatom Nitzschia laevis for Eicosapentaenoic Acid Production: Physiological Study and Process Optimization. Biotechnol. Progress 2002, 18:21-28.
100. Wen Z.Y., Chen F. Perfusion Culture of the Diatom Nitzschia laevis for Ultra-high Yield of Eicosapentaenoic Acid. Process Biochem. 2002, 38:523-529.
101. Wen Z.Y., Chen F. Heterotrophic Production of Eicosapentaenoic Acid by Microalgae. Biotechnol. Adv. 2003, 21:273-294.
102. Wen Z.Y., Jiang Y., Chen F. High Cell Density Culture of the Diatom Nitzschia laevis for Eicosapentaenoic Acid Production: Fed-Batch Development. Process Biochem. 2002, 37:1447-1453.
103. Werner D. Silicate Metabolism. The Biology of Diatom 1977, 111-149. Blackwell Scientific, Oxford. D. Werner (Ed.).
104. Xu X.Q., Beardall J. Effect of Salinity on Fatty Acid Composition of a Green Microalga from an Antarctic Hypersaline Lake. Phytochem. 1997, 45:655-658.
105. Yano Y., Nakayama A., Yoshida K. Distribution of Polyunsaturated Fatty Acids in Bacteria Present in Intestines of Deep-sea Fish and Shallow-sea Poikilothermic Animals. Appl. Env. Microbiol. 1997, 63:2572-2577.
106. Yazawa K. Production of Eicosapentaenoic Acid from Marine Bacteria. Lipids 1996, 31:S297-S300.
107. Yazawa K., Araki K., Okazaki N., Watanabe K., Ishikawa C., Inoue A., Numao N., Kondo K. Production of Eicosapentaenoic Acid by Marine-Bacteria. J. Biochem. 1988, 103:5-7.
108. Yongmanitchai W.C., Ward O.P. Omega-3 Fatty-Acids-Alternative Sources of Production. Process Biochem. 1989, 24:117-125.
109. Yongmanitchai W., Ward O.P. Growth of and Omega-3-Fatty-Acid Production by Phaeodactylum tricornutum under Different Culture Conditions. Appl. Env. Microbiol. 1991, 57:419-425.
110. Yongmanitchai W., Ward O.P. Growth and Eicosapentaenoic Acid Production by Phaeodactylum tricornutum in Batch and Continuous Culture Systems. J. Am. Oil Chem. Soc. 1992, 69:584-590.
111. Yu R., Yamada A., Watanabe K., Yazawa K., Takeyama H., Matsunaga T., Kurane R. Production of Eicosapentaenoic Acid by a Recombinant Marine Cyanobacterium, Synechococcus sp. Lipids 2000, 35:1061-1064.
112. Zaslavskaia L.A., Lippmeier J.C., Shih C., Ehrhardt D., Grossman A.R., Apt K.E. Trophic Obligate Conversion of an Photoautotrophic Organism through Metabolic Engineering. Science 2001, 292:2073-2075.
113. Zittelli G.C., Lavista F., Bastianini A., Rodolfi L., Vincenzini M., Tredici M.R. Production of Eicosapentaenoic Acid by Nannochloropsis sp. Cultures in Outdoor Tubular Photobioreactors. J. Biotechnol. 1999, 70:299-312.
114. Zittelli G.C., Pastorelli R., Tredici M.R. A Modular Flat Panel Photobioreactor (MFPP) for Indoor Mass Cultivation of Nannochloropsis sp. under Artificial Illumination. J. Appl. Phycol. 2000, 12:521-526.