Photolimitation and photoinhibition as factors determining optimal dilution rate to produce eicosapentaenoic acid from cultures of the microalga Isochrysis galbana

Applied Microbiology and Biotechnology

Volumn 50, Issue 2, 1998, Pages 199-205

  Fernandez Sevilla J M ^a   Molina Grima, E ^a   Garcia Camacho F ^a   Acien Fernandez F G ^a   Sanchez Perez J A ^a  

^a UNIVERSITY OF ALMERÍA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ICOSAPENTAENOIC ACID;

ALGA; ARTICLE; BIOMASS; CONTINUOUS CULTURE; DILUTION; DRUG MANUFACTURE; GROWTH RATE; PHOTOCHEMISTRY;

ISOCHRYSIS GALBANA;

EID: 0031715240     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051277     Document Type: Article

References (21)

1. Bajpai P, Bajpai PK (1993) Eicosapentaenoic acid (EPA) production from microorganisms: a review. J Biotechnol 30: 161-183
2. Brown MR, Dunstan GA, Jeffrey SW, Volkman JK, Barrett SM, Leroi JM (1993) The influence of irradiance on the biochemical composition of the prymnesiophyte Isochrysis sp. (clone t-iso). J Phycol 29: 601-612
3. Harrison PJ, Thompson PA, Calderwood GS (1990) Effects of nutrient and light limitation on the biochemical composition of phytoplankton. J Appl Phycol 2: 45-56
4. Hodgson PA, Henderson RJ, Sargent JR, Leftley JW (1991) Patterns of variation in the lipid class and fatty acid composition of Nannochloropsis oculata (Eustigmaphyceae) during batch culture. I. The growth cycle. J Appl Phycol 3: 169-181
5. Lassen C, Jorgensen B (1994) A fiber-optic irradiance microsensor (cosine collector): application for in situ measurements of absorption coefficients in sediments and microbial mats. FEMS Microbiol Ecol 15: 321-336
6. Lepage G, Roy C (1984) Improved recovery of fatty acid through direct transesterification without prior extraction or purification. J Lipid Res 25: 1391-1396
7. López Alonso D, Belarbi H, Rodríguez Ruiz J, Segura del Castillo CI, Giménez Giménez A (1997) Detailed acyl-lipid composition of three microalgae. Phytochemistry (in press)
8. Molina Grima E, Sánchez Pérez JA, García Camacho F, García Sánchez JL, López Alonso D (1993) n-3 PUFA productivity in chemostat cultures of microalgae. Appl Microbiol Biotechnol 38: 599-605
9. Molina Grima E, García Camacho F, Sánchez Pérez JA, Fernández Sevilla JM, Acién Fernández FG, Contreras Gómez A (1994a) A Mathematical model of microalgal growth in light limited chemostat culture. J Chem Technol Biotechnol 61: 167-173
10. Molina Grima E, Sánchez Pérez JA, García Camacho F, Fernández Sevilla JM, Acién Fernández FG (1994b) Effect of growth rate on EPA and DHA content of Isochrysis galbana in chemostat culture. Appl Microbiol Biotechnol 41: 23-27
11. Molina Grima E, Sánchez Pérez JA, García Camacho F, García Sánchez JL, Acién Fernández FG, López Alonso D (1994c) Outdoor culture of Isochrysis galbana ALII-4 in a closed tubular photobioreactor. J Biotechnol 37: 159-166
12. Molina Grima E, Sánchez Pérez JA, García Camacho F, García Sánchez JL, Fernández Sevilla JM (1995) Variation of fatty acid profile with solar cycle in outdoor chemostat culture of Isochrysis galbana ALII-4. J Appl Phycol 7: 129-134
13. Molina Grima E, Fernández Sevilla JM, Sánchez Pérez JA, García Camacho F (1996) A study on simultaneous photolimitation and photoinhibition in dense microalgal cultures taking into account incident and averaged irradiances. J Biotechnol 45: 59-69
14. Rabe AE, Benoit A (1962) Mean light intensity-a useful concept in correlating growth rates of dense cultures of microalgae. Biotechnol Bioengng 4: 377-390
15. Radwan SS (1991) Sources of C20-polyunsaturated fatty acids for biochemical use. Appl Microbiol Biotechnol 35: 421-430
16. Rambjor GS, Walen AI, Windsor SL, Harris WS (1996) Eicosapentaenoic acid is primarily responsible for hypotriglyceridemic effect of fish oil in humans. Lipids 31 (Suppl S): 45-49
17. Renaud SM, Parry DL, Luong-Van, Thinh Kuo C, Radovan A, Sammy N (1991) Effect of light intensity on the proximate biochemical and fatty acid composition of Isochrysis sp. And Nannochloropsis oculata for use in tropical aquaculture. J Appl Phycol 3: 43-53
18. Sánchez Pérez JA (1994) n-3 polyunsaturated fatty acid productivity of the marine microalga Isochrysis galbana. Growth conditions and phenotypic selection. J Appl Phycol 6: 475-478
19. Saoudihelis L, Dubacq JP, Marty Y, Samain JF, Gudin C (1994) Influence of growth rate on pigment and lipid composition of the microalga Isochrysis aff galbana clone t-iso. J Appl Phycol 6: 315-322
20. Sukenik A, Carmeli Y, Berner T (1989) Regulation of fatty acid composition by irradiance level in the eustigmatophyte Nannochloropsis sp. J Phycol 25: 686-692
21. Thompson PA, Harrison PJ, Whyte JNC (1990) Influence of irradiance on the fatty acid composition of phytoplankton. J Phycol 26: 278-288