First insights into improvement of eicosapentaenoic acid content in Phaeodactylum tricornutum (Bacillariophyceae) by induced mutagenesis

Journal of Phycology

Volumn 32, Issue 2, 1996, Pages 339-345

  López Alonso, Diego ^a   Segura Del Castillo, Clara I ^a   Molina Grima, Emilio ^a   Cohen, Zvi ^b  

^a UNIVERSITY OF ALMERÍA   (Spain)

^b BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

Bacillariophyceae; eicosapentaenoic acid; EPA; microalgae; mutation; Phaeodactylum tricornutum; selection; strain development; strain improvement

Indexed keywords

BACILLARIOPHYCEAE; PHAEODACTYLUM TRICORNUTUM;

EID: 0010383006     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0022-3646.1996.00339.x     Document Type: Article

References (42)

1. Baltz, R. H. 1986. Strain improvements: introduction. In Demain, A. L. & Solomon, N. A. [Eds.] Manual of Industrial Microbiology and Biotechnology. American Society for Microbiology, Washington, D.C., p. 154.
2. Barclay, W. R., Meager, K. M. & Abril, J. R. 1994. Heterotrophic production of long chain omega-3 fatty acids utilizing algae and algae-like microorganisms. J. Appl. Phycol. 6:123-9.
3. Borowitzka, M. 1988. Fats, oils and hydrocarbons. In Borowitzka, M. A. & Borowitzka, L. J. [Eds.] Micro-algal Biotechnology. Cambridge University Press, Cambridge, pp. 257-87.
4. Brown, L., Dunahay, T. & Jarvis, E. 1990. Applications of genetics to microalgae production. Dev. Ind. Microbiol 31:271-4.
5. Bronsgeest-Schoute, H. C., van Gent, C. M., Luten, J. B. & Ruiter, A. 1981. The effects of various intakes of ω3 fatty acids on the blood lipid composition in healthy human subjects. Am. J. Clin. Nutr. 34:1752-7.
6. Carlton, B. C. & Brown, B. J. 1981. Gene mutation. In Manual of Methods for General Bacteriology, Ch. 13. American Society for Microbiology, Washington, D.C., pp. 222-42.
7. Cohen, Z. 1994. Production potential of eicosapentaenoic acid by Monodus subterraneus. J. Am. Oil Chem. Soc. 71:941-5.
8. Cohen, Z. & Heimer, Y. M. 1992. Production of polyunsaturated fatty acids (EPA, ARA and GLA) by the microalgae Porphyridium and Spirulina. In Kyle, D. J. & Ratledge, C. [Eds.] Industrial Applications of Single Cell Oils. American Oils Chemists' Society, Champaign, Illinois, pp. 243-73.
9. Cohen, Z., Norman, H. A. & Heimer, Y. M. 1993. Potential use of substituted pyridazinones for selecting polyunsaturated fatty acid overproducing cell lines of algae. Photochemistry 32: 259-64.
10. _ 1995. Microalgae as a source of ω3 fatty acids. World Rev. Nutr. Diet. 77:1-31.
11. Crueger, W. & Crueger, A. 1990. A Textbook of Industrial Microbiology. Sinauer Associates, Sunderland, Massachusets, 357 pp.
12. de la Noue, J. & de Paw, N. 1988. The potential of microalgae biotechnology: a review of production and uses of microalgae. Biotech. Adv. 6:725-70.
13. Dyerberg, J. 1986. Linolenate-derived polyunsaturated fatty acids and prevention of atherosclerosis. Nutr. Rev 44:125-34.
14. Elander, R. P & Demain, A. L. 1981. Genetics of microorganisms in relation to industrial requirements. In Rehm, H-J. & Reed, G. [Eds.] Biotechnology. Vol. 1. Microbial Fundamentals. Verlag Chemie, Weinheim, pp. 235-77.
15. Iacono, J. M. & Dougherty, R. M. 1993. Effects of polyunsaturated fats on blood pressure. Annu. Rev. Nutr. 13:243-60.
16. Jareonkitmongkol, S., Shimizu, S. & Yamada, H. 1993. Production of an eicosapentaenoic acid-containing oil by a Δ12 desaturase-defective mutant of Morteriella alpina 1S-4. J. Am. Oil Chem. Soc. 70:119-23.
17. Kyle, D. 1989. Market applications for microalgae. J. Am. Oil Chem Soc. 66:648-53.
18. _ 1991. Specialty oils from microalgae: new perspectives. In Rattray, J. [Ed.] Biotechnology of Plant Fats and Oils. American Oil Chemists' Society, Champaign, Illinois, pp. 130-43.
19. López Alonso, D., Molina Grima, E., Sánchez Pérez, J. A., García Sánchez, J. L. & García Camacho, F. 1992. Isolation of clones of Isochrysis galbana rich in eicosapentaenoic acid. Aquaculture 102:363-71.
20. López Alonso, D., Sánchez Pérez, J. A., García Sánchez, J. L., García Camacho, F. & Molina Grima, E. 1993. Improvement of the EPA content in isolates of Isochrysis galbana. J. Mar. Biotech. 1:1417-9.
21. López Alonso, D., Segura del Castillo, C. I., García Sánchez, J. L., Sánchez Pérez, J. A. & García Camacho, F. 1994. Quantitative genetics of fatty acid variation in Isochrysis galbana (Prymnesiophyceae) and Phaeodactylum tricornutum (Bacillariophyceae). J. Phycol. 30:553-8.
22. Mann, J. E. & Myers, J. 1968. On pigments, growth, and photosynthesis of Phaeodactylum tricornutum. J. Phycol 4:349-55.
23. Molina Grima, E., García Camacho, F., Sánchez Pérez, J. A., Urda Cardona, J., Acién Fernández, F. G. & Fernández Sevilla, J. M. 1994a. Outdoor chemostat culture of Phaeodactylum tricornutum UTEX 640 in a tubular photobioreactor. Biotechnol. Appl. Biochem. 20:279-90.
24. Molina Grima, E., Sánchez Pérez, J. A., García Camacho, F., Fernández Sevilla, F. J. & Acién Fernández, F. G. 1994b. Effect of growth rate on the eicosapentaenoic acid and docosahexaenoic acid content of Isochrysis galbana in chemostat culture. Appl. Microbiol. Biotechnol. 41:23-7.
25. Molina Grima, E., Sánchez Pérez, J. A., García Camacho, F., Fernández Sevilla, J. M., Acién Fernández, F. G. & Urda Cardona, J. 1995. Biomass and EPA productivities from an outdoor batch culture of Phaeodactylum tricornutum UTEX 640 in an airlift tubular photobioreactor. Appl. Microbiol. Biotechnol. 42:658-63.
26. Molina Grima, E., Sánchez Pérez, J. A., García Camacho, F., García Sánchez, J. L., Acién Fernández, F. G. & López Alonso, D. 1994e. Outdoor culture of Isochrysis galbana ALII-4 in a closed tubular photobioreactor. J. Biotechnol. 37:159-66.
27. Nettleton, J. A. 1993. Are n-3 fatty acids essential nutrients for fetal and infant development? J. Am. Diet. Assoc. 93:58-64.
28. O'Brien, D. J., Kurantz, M. J. & Kwoczak, R. 1993. Production of eicosapentaenoic acid by the filamentous fungus Pythium irregulare. Appl. Microbiol. Biotechnol. 40:211-4.
29. Ohta, S., Chang, T., Aozasa, O., Kondo, M. & Miyata, H. 1992. Sustained production of arachidonic and eicosapentaenoic acids by the red alga Porphyridium purpureum cultured in a light/dark cycle. J. Ferment. Bioeng. 74:398-402.
30. Plakas, S. M. & Guarino, A. M. 1986. Omega-3 fatty acids and fish oils: is the news all good? Proceedings of the Eleventh Annual Tropical and Subtropical Fisheries Conference of the American. Texas Agricultural Extension Service, Marine Advisory Service Program, pp. 275-83.
31. Queener, S. W. & Lively, D. H. 1986. Screening and selection for strain improvement. In Demain, A. L. & Solomon, N. A. [Eds.] Manual of Industrial Microbiology and Biotechnology. American Society for Microbiology, Washington, D.C., pp. 155-69.
32. Radmer, R. J. 1990. Omega-3 fatty acids from algae. In Lee, R. S. & Karel, M. [Eds.] Omega-3 Fatty Acids in Health and Disease. Marcel Dekker, New York, pp. 211-4.
33. Ratledge, C. 1989. Microbial routes to lipids. Biochem. Soc. Trans. 17:1139-41.
34. Rowlands, R. T. 1984. Industrial strain improvement: mutagenesis and random screening procedures. Enzyme Microb. Technol. 6:3-10.
35. Seto, A., Wang, H. L. & Hesseltine, C. W. 1984. Culture conditions affect eicosapentaenoic acid content of Chlorella minutissima. J. Am. Oil. Chem. Soc. 61:892-4.
36. Simopoulos, A. P. 1991. Omega-3 fatty acids in health and disease and in growth and development. Am. J. Clin. Nutr. 54: 438-63.
37. Sokal, R. R. & Rohlf, F. J. 1981. Biometry. W. H. Freeman, San Francisco, 859 pp.
38. Suzuki, N., Inoue, A., Shikano, M., Yazawa, K. & Kondo, K. 1991. Effect of arginine on the production of eicosapentaenoic acid (EPA) in EPA-elaborating bacterium SCRC-2738. Bull. Jap. Soc. 57:1407.
39. Veloso, V., Reis, A., Gouveia, L., Fernandes, H. L., Empis, J. A. & Novais, J. M. 1991. Lipid production by Phaeodactylum tricornutum. Bioresource Technol. 38:115-9.
40. Yongmanitchai, W. & Ward, O. P. 1989. Omega-3 fatty acids: alternative source of production. Process Biochem. 24:117-25.
41. _ 1991a. Screening of algae for potential alternative sources of eicosapentaenoic acid. Phytochemistry 30:2963-7.
42. _ 1991b. Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions. Appl Environ Microbiol. 57:419-25.