Comparative sensitivity analyses of quantitative polymerase chain reaction and flow cytometry in detecting cellular microchimerism in murine tissues

Journal of Immunological Methods

Volumn 406, Issue , 2014, Pages 74-82

  Thiele, Kristin ^a   Holzmann, Carsten ^b   Solano, Maria Emilia ^a   Zahner, Gunther ^a   Arck, Petra Clara ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^b UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

CD45.1; EGFP; Flow cytometry; Microchimerism; QPCR; Sensitivity

Indexed keywords

CD11B ANTIGEN; CD19 ANTIGEN; CD3 ANTIGEN; CD45 ANTIGEN; CD45.1 ANTIGEN; CD45.2 ANTIGEN; ENHANCED GREEN FLUORESCENT PROTEIN; NATURAL CYTOTOXICITY TRIGGERING RECEPTOR 1; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL COUNT; CELL SUSPENSION; CELLULAR MICROCHIMERISM; CONTROLLED STUDY; DNA CONTENT; FEMALE; FLOW CYTOMETRY; IN VITRO STUDY; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MALE; MICROCHIMERISM; MOUSE; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TARGETING; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SPLEEN CELL;

MURINAE; MUS; MUS MUSCULUS;

CD45.1; EGFP; FLOW CYTOMETRY; MICROCHIMERISM; QPCR; SENSITIVITY;

ANIMALS; CHIMERISM; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; LEUKOCYTES; LIMIT OF DETECTION; LYMPH NODES; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; REAL-TIME POLYMERASE CHAIN REACTION; SPLEEN;

EID: 84900849874     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2014.03.009     Document Type: Article

References (40)

1. Adams K.M., Nelson J.L. Microchimerism: an investigative frontier in autoimmunity and transplantation. J. Am. Med. Assoc. 2004, 291:1127.
2. Basu S., Ray A., Dittel B.N. Differential representation of B cell subsets in mixed bone marrow chimera mice due to expression of allelic variants of CD45 (CD45.1/CD45.2). J. Immunol. Methods 2013, 396(1-2):163-167.
3. Bloch E.M., Reed W.F., Lee T.-H., Montalvo L., Shiboski S., Custer B., Barcellos L. Male microchimerism in peripheral blood leukocytes from women with multiple sclerosis. Chimerism 2011, 2:6.
4. Campana D., Coustan-Smith E. Detection of minimal residual disease in acute leukemia by flow cytometry. Cytometry 1999, 38:139.
5. Chalfie M., Euskirchen G., Prasher D.C. Green fluorescent protein as a marker for gene expression. Science 1994, 263(80-):802.
6. Dawe G.S., Xiao Z.-C. Cell migration from baby to mother. Cell Adhes. Migr. 2007, 1:19.
7. Deak E., Göttig S., Rüster B., Paunescu V., Seifried E., Gille J., Henschler R. Bone marrow derived cells in the tumour microenvironment contain cells with primitive haematopoietic phenotype. J. Cell. Mol. Med. 2010, 14:1946.
8. Dutta P., Burlingham W.J. Microchimerism: tolerance vs. sensitization. Curr. Opin. Organ Transplant. 2011, 16.
9. Ekins R., Edwards P. Point on the meaning of "sensitivity". Clin. Chem. 1997, 43:1824.
10. Gadi V.K., Nelson J.L. Fetal microchimerism in breast cancer. Cancer Res. 2007, 67:9035.
11. Gadi V.K., Nelson J.L., Boespflug N., Guthrie K., Kuhr C. Soluble donor DNA concentrations in recipient serum correlates with pancreas-kidney rejection. Clin. Chem. 2006, 52:379.
12. Gross H.J., Verwer B., Houck D., Recktenwald D. Detection of rare cells at a frequency of one per million by flow cytometry. Cytometry 1993, 14:519.
13. Heim R., Tsien R.Y. Improved green fluorescence. Nature 1995, 373:663.
14. Henri S., Poulin L.F., Tamoutounour S., Ardouin L., Guilliams M., de Bovis B., Devilard E., Viret C., Azukizawa H., Kissenpfennig A., Malissen B. CD207+ CD103+ dermal dendritic cells cross-present keratinocyte-derived antigens irrespective of the presence of Langerhans cells. J. Exp. Med. 2010, 207:189.
15. Inouye S., Tsuji F.I. Evidence for redox forms of the Aequorea green fluorescent protein. FEBS Lett. 1994, 351:211.
16. Joshi M.U., Pittman H.K., Haisch C.E., Verbanac K.M. Real-Time PCR to determine transgene copy number and to quantitate the biolocalization of adoptively transferred cells from EGFP-transgenic mice. Biotechniques 2008, 45:247.
17. Liegeois A., Escourrou J., Ouvre E., Charreire J. Microchimerism: a stable state of low-ratio proliferation of allogeneic bone marrow. Transplant. Proc. 1977, 9:273.
18. Maloney S., Smith A., Furst D.E., Myerson D., Rupert K., Evans P.C., Nelson J.L. Microchimerism of maternal origin persists into adult life. J. Clin. Invest. 1999, 104:41.
19. Marleau A.M., Greenwood J.D., Wei Q., Singh B., Croy B.A. Chimerism of murine fetal bone marrow by maternal cells occurs in late gestation and persists into adulthood. Lab. Invest. 2003, 83:673.
20. Mold J.E., Michaëlsson J., Burt T.D., Muench M.O., Beckerman K.P., Busch M.P., Lee T.-H., Nixon D.F., McCune J.M. Maternal alloantigens promote the development of tolerogenic fetal regulatory T cells in utero. Science 2008, 322:1562.
21. Nelson J.L. Maternal-fetal immunology and autoimmune disease: is some autoimmune disease auto-alloimmune or allo-autoimmune?. Arthritis Rheum. 1996, 39:191.
22. Nelson J.L., Furst D.E., Maloney S., Gooley T., Evans P.C., Bean M.A., Ober C., Smith A.J., Bianchi D.W. Microchimerism and HLA-compatible relationships of pregnancy in women with scleroderma. Lancet 1998, 351:559.
23. Okabe M., Ikawa M., Kominami K., Nakanishi T., Nishimune Y. 'Green mice' as a source of ubiquitous green cells. FEBS Lett. 1997, 407:313.
24. Østensen M., Villiger P.M., Förger F. Interaction of pregnancy and autoimmune rheumatic disease. Autoimmun. Rev. 2012, 11:A437.
25. Reed W., Lee T.H., Norris P.J., Utter G.H., Busch M.P. Transfusion associated microchimerism: a new complication of blood transfusions in severely injured patients. Semin. Hematol. 2007, 44:24.
26. Sambrook J., Russell D.W. Molecular Cloning: A Laboratory Manual 2001, Cold Spring Harbor Laboratory Press, New York. Third edition.
27. Schreiner T., Prochnow-Calzia H., Maccari B., Erne E., Kinzler I., Wölpl A., Wiesneth M. Chimerism analysis after allogeneic bone marrow transplantation with nonradioactive RFLP and PCR-AFLP using the same DNA. J. Immunol. Methods 1996, 196:93.
28. Shimomura O., Saiga Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan Aequorea. J. Cell. Comp. Physiol. 1962, 59:223.
29. Srivatsa B., Srivatsa S., Bianchi D.W. Maternal cell microchimerism in newborn tissues. J. Pediatr. 2003, 142:31.
30. Starzl T.E. Chimerism and tolerance in transplantation. Proc. Natl. Acad. Sci. 2004, 101:14607.
31. Sun W., Wang Q., Zhang L., Pan J., Zhang M., Lu G., Yao H., Wang J., Cao X. TGF-β1 gene modified immature dendritic cells exhibit enhanced tolerogenicity but induce allograft fibrosis in vivo. J. Mol. Med. 2002, 80:514.
32. The Jackson Laboratory GFP expressing lymphocyte populations [WWW document] URL. http://jaxmice.jax.org/research/gfp_expressing_lymphocyte_populations.pdf.
33. The Jackson Laboratory Fluorescent proteins (generic GFP) [WWW document] URL. http://jaxmice.jax.org/protocolsdb/f?p=116:2:2447686549735754::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:6125,003291.
34. Visser J.W.M., Martens A.C.M., Hagenbeck A. Detection of minimal residual disease in acute leukemia by flow cytometry. Ann. N. Y. Acad. Sci. 1986, 468:268.
35. Wen T., Besse J.A., Mingler M.K., Fulkerson P.C., Rothenberg M.E. Eosinophil adoptive transfer system to directly evaluate pulmonary eosinophil trafficking in vivo. Proc. Natl. Acad. Sci. 2013, 110:6067.
36. Wienecke J., Hebel K., Hegel K.J., Pierau M., Brune T., Reinhold D., Pethe A., Brunner-Weinzierl M.C. Pro-inflammatory effector Th cells transmigrate through anti-inflammatory environments into the murine fetus. Placenta 2012, 33:39.
37. Xu H., Exner B., Chilton P., Schanie C., Ildstad S.T. CD45 congenic bone marrow transplantation: evidence for T cell-mediated immunity. Stem Cells 2004, 22:1039.
38. Yan Z., Aydelotte T., Gadi V.K., Guthrie K.A., Nelson J.L. Acquisition of the rheumatoid arthritis HLA shared epitope through microchimerism. Arthritis Rheum. 2011, 63:640.
39. Zebedee S.L., Barritt D.S., Raschke W.C. Comparison of mouse Ly5a and Ly5b leucocyte common antigen alleles. Dev. Immunol. 1991, 1:243.
40. Zhou X., Jeker L.T., Fife B.T., Zhu S., Anderson M., McManus M.T., Blueston J.A. Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity. J. Exp. Med. 2008, 205:1983.