Design of thermostable rhamnogalacturonan lyase mutants from Bacillus licheniformis by combination of targeted single point mutations

Applied Microbiology and Biotechnology

Volumn 98, Issue 10, 2014, Pages 4521-4531

  Silva, Inês R ^a   Jers, Carsten ^a   Otten, Harm ^b   Nyffenegger, Christian ^a   Larsen, Dorte M ^a   Derkx, Patrick M F ^c   Meyer, Anne S ^a   Mikkelsen, Jørn D ^a   Larsen, Sine ^b  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c CHR HANSEN A S   (Denmark)

Author keywords

Bacillus licheniformis; Bacillus subtilis expression; Crystal structure; Polysaccharide lyase family 11; Protein engineering; RGI lyase

Indexed keywords

AMINO ACIDS; BACTERIOLOGY; BIOCHEMICAL ENGINEERING; CRYSTAL STRUCTURE; GENETIC ENGINEERING; THERMODYNAMIC STABILITY;

BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS; FAMILY 11; PROTEIN ENGINEERING; RGI LYASE;

ENZYMES;

LYASE; RHAMNOGALACTURONAN I LYASE; UNCLASSIFIED DRUG; MUTANT PROTEIN; PECTIN; POLYSACCHARIDE LYASE; RHAMNOGALACTURONAN I;

AMINO ACID; BACTERIUM; BIOENGINEERING; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVITY; MUTATION; POLYSACCHARIDE; PROTEIN; YEAST;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS; BACTERIUM MUTANT; CIRCULAR DICHROISM; CONSENSUS DEVELOPMENT; ENZYME GLYCOSYLATION; HALF LIFE TIME; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POINT MUTATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN STABILITY; THERMOSTABILITY; BACILLUS; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HEAT; METABOLISM; PICHIA; PROTEIN CONFORMATION; RADIATION RESPONSE;

BACILLUS LICHENIFORMIS; BACILLUS SUBTILIS; PICHIA PASTORIS;

AMINO ACID SUBSTITUTION; BACILLUS; CIRCULAR DICHROISM; ENZYME STABILITY; HOT TEMPERATURE; MUTANT PROTEINS; PECTINS; PICHIA; POINT MUTATION; POLYSACCHARIDE-LYASES; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN STABILITY;

EID: 84900808594     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5483-8     Document Type: Article

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