Determination of proteinligand binding constants of a cooperatively regulated tetrameric enzyme using electrospray mass spectrometry

ACS Chemical Biology

Volumn 9, Issue 1, 2014, Pages 218-226

  Cubrilovic, Dragana ^a   Haap, Wolfgang ^b   Barylyuk, Konstantin ^a   Ruf, Armin ^b   Badertscher, Martin ^a   Gubler, Marcel ^b   Tetaz, Tim ^b   Joseph, Catherine ^b   Benz, Jorg ^b   Zenobi, Renato ^a  

^a ETH ZURICH   (Switzerland)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; ESTERASE INHIBITOR; FRUCTOSE 1,6 BISPHOSPHATASE INHIBITOR; FRUCTOSE BISPHOSPHATASE; GLUCOSE; LIGAND; PROTEIN; SULFONYLUREA; TETRAMER; UNCLASSIFIED DRUG; ENZYME INHIBITOR;

ALLOSTERISM; ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DISSOCIATION CONSTANT; DRUG CONFORMATION; DRUG POTENCY; DRUG TARGETING; ELECTROSPRAY MASS SPECTROMETRY; GLYCEMIC CONTROL; HUMAN; IC 50; LIGAND BINDING; LIVER; MEASUREMENT; MOLECULAR DYNAMICS; NANO ELECTROSPRAY IONIZATION MASS SPECTROMETRY; NON INSULIN DEPENDENT DIABETES MELLITUS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; STOICHIOMETRY; X RAY CRYSTALLOGRAPHY; CHEMICAL STRUCTURE; CHEMISTRY; DRUG DEVELOPMENT; METABOLISM; METHODOLOGY; MOLECULAR LIBRARY; PROTEIN BINDING; PROTEIN MULTIMERIZATION;

ALLOSTERIC REGULATION; CRYSTALLOGRAPHY, X-RAY; DRUG DISCOVERY; ENZYME INHIBITORS; FRUCTOSE-BISPHOSPHATASE; LIGANDS; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN MULTIMERIZATION; SMALL MOLECULE LIBRARIES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84900434296     PISSN: 15548929     EISSN: 15548937     Source Type: Journal    
DOI: 10.1021/cb4007002     Document Type: Article

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