Quasi-spherical microwells on superhydrophobic substrates for long term culture of multicellular spheroids and high throughput assays

Biomaterials

Volumn 35, Issue 23, 2014, Pages 6060-6068

  Liu, Tianqing ^a   Winter, Marnie ^a   Thierry, Benjamin ^a  

^a UNIVERSITY OF SOUTH AUSTRALIA   (Australia)

Author keywords

Drug screening; Ice lithography; Imaging flow cytometry; Microwells; Multicellular tumour spheroids

Indexed keywords

ASSAYS; BIOLOGICAL MATERIALS; FLOW CYTOMETRY; HYDROPHOBICITY; RESEARCH LABORATORIES; SUBSTRATES; THROUGHPUT; TUMORS;

DRUG SCREENING; HETEROGENEOUS POPULATIONS; HIGH THROUGHPUT SCREENING; ICE LITHOGRAPHY; MICROWELLS; MULTICELLULAR TUMOUR SPHEROIDS; PHYSIOLOGICAL ENVIRONMENT; TECHNOLOGICAL CHALLENGES;

DIAGNOSIS;

CYTOKERATIN; ICE; SULFURIC ACID; UVOMORULIN; VIMENTIN; WATER; BAYSILON; BIOMATERIAL; DIMETICONE;

ARTICLE; BIOCOMPATIBILITY; CELL POPULATION; CELL STRUCTURE; CELL VIABILITY ASSAY; CONTACT ANGLE; CONTROLLED STUDY; CYTOTOXICITY ASSAY; FLOW CYTOMETRY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; HYDROPHOBICITY; LAB ON A CHIP; MCF 7 CELL LINE; MICROTECHNOLOGY; MICROWELL PLATE; MOLECULAR IMAGING; MONOLAYER CULTURE; MTT ASSAY; MULTICELLULAR SPHEROID; PHENOTYPIC VARIATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; TUMOR SPHEROID; BATCH CELL CULTURE; CELL CULTURE; CHEMISTRY; DEVICE FAILURE ANALYSIS; DEVICES; DRUG SCREENING; EQUIPMENT DESIGN; SURFACE PROPERTY; SYNTHESIS; TISSUE MICROARRAY;

BATCH CELL CULTURE TECHNIQUES; BIOCOMPATIBLE MATERIALS; CELLS, CULTURED; DIMETHYLPOLYSILOXANES; DRUG EVALUATION, PRECLINICAL; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; FLOW CYTOMETRY; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MCF-7 CELLS; SURFACE PROPERTIES; TISSUE ARRAY ANALYSIS;

EID: 84900021928     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2014.04.047     Document Type: Article

References (34)

1. Kim J., Stein R., O'Hare M. Three-dimensional invitro tissue culture models of breast cancer - a review. Breast Cancer Res Treat 2004, 85:281-291.
2. Hirschhaeuser F., Menne H., Dittfeld C., West J., Mueller-Klieser W., Kunz-Schughart L.A. Multicellular tumor spheroids: an underestimated tool is catching up again. JBiotechnol 2010, 148:3-15.
3. Lin R., Chang H. Recent advances in three-dimensional multicellular spheroid culture for biomedical research. Biotechnol J 2008, 3:1172-1184.
4. Charnley M., Textor M., Khademhosseini A., Lutolf M. Integration column: microwell arrays for mammalian cell culture. Integr Biol 2009, 1:625-634.
5. Choi Y.Y., Chung B.G., Lee D.H., Khademhosseini A., Kim J.-H., Lee S.-H. Controlled-size embryoid body formation in concave microwell arrays. Biomaterials 2010, 31:4296-4303.
6. Wong S.F., No D.Y., Choi Y.Y., Kim D.S., Chung B.G., Lee S.-H. Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model. Biomaterials 2011, 32:8087-8096.
7. Jeong G.S., Song J.H., Kang A.R., Jun Y., Kim J.H., Chang J.Y., et al. Surface tension-mediated, concave-microwell arrays for large-scale, simultaneous production of homogeneously sized embryoid bodies. Adv Healthc Mater 2013, 2:119-125.
8. Lee K.H., No D.Y., Kim S.-H., Ryoo J.H., Wong S.F., Lee S.-H. Diffusion-mediated in situ alginate encapsulation of cell spheroids using microscale concave well and nanoporous membrane. Lab Chip 2011, 11:1168-1173.
9. Chao S.-h., Carlson R., Meldrum D.R. Rapid fabrication of microchannels using microscale plasma activated templating (PLAT) generated water molds. Lab Chip 2007, 7:641-643.
10. Park J., Hwang C., Lee S.-H. Ice-lithographic fabrication of concave microwells and a microfluidic network. Biomed Microdevices 2009, 11:129-133.
11. Jeong G.S., Jun Y., Song J.H., Shin S.H., Lee S.-H. Meniscus induced self organization of multiple deep concave wells in a microchannel for embryoid bodies generation. Lab Chip 2012, 12.
12. Kang E., Choi Y.Y., Jun Y., Chung B.G., Lee S.-H. Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval. Lab Chip 2010, 10:2651-2654.
13. Kuo C.-T., Chiang C.-L., Yun-Ju Huang R., Lee H., Wo A.M. Configurable 2D and 3D spheroid tissue cultures on bioengineered surfaces with acquisition of epithelial-mesenchymal transition characteristics. NPG Asia Mater 2012, 4:e27.
14. Kim C., Bang J.H., Kim Y.E., Lee S.H., Kang J.Y. On-chip anticancer drug test of regular tumor spheroids formed in microwells by a distributive microchannel network. Lab Chip 2012, 12:4135-4142.
15. Denizot F., Lang R. Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. JImmunol Methods 1986, 89:271-277.
16. Odot J., Albert P., Carlier A., Tarpin M., Devy J., Madoulet C. Invitro and invivo anti-tumoral effect of curcumin against melanoma cells. Int J Cancer 2004, 111:381-387.
17. de Givenchy E.T., Amigoni S., Martin Cd, Andrada G., Caillier L., Géribaldi S., et al. Fabrication of superhydrophobic PDMS surfaces by combining acidic treatment and perfluorinated monolayers. Langmuir 2009, 25:6448-6453.
18. Xu Y., Xie F., Qiu T., Xie L., Xing W., Cheng J. Rapid fabrication of a microdevice with concave microwells and its application in embryoid body formation. Biomicrofluidics 2012, 6:016504.
19. Lee B.R., Hwang J.W., Choi Y.Y., Wong S.F., Hwang Y.H., Lee D.Y., et al. In situ formation and collagen-alginate composite encapsulation of pancreatic islet spheroids. Biomaterials 2012, 33:837-845.
20. Jun Y., Kang A.R., Lee J.S., Jeong G.S., Ju J., Lee D.Y., et al. 3D co-culturing model of primary pancreatic islets and hepatocytes in hybrid spheroid to overcome pancreatic cell shortage. Biomaterials 2013, 34:3784-3794.
21. Tu T.-Y., Wang Z., Bai J., Sun W., Peng W.K., Huang R.Y.-J., et al. Rapid prototyping of concave microwells for the formation of 3D multicellular cancer aggregates for drug screening. Adv Healthc Mater 2013, 10.1002/adhm.201300151.
22. Hwang J., Lee B., Jung M., Jung H., Hwang Y., Kim M., et al. Functional clustering of pancreatic islet cells using concave microwell array. Macromol Res 2011, 19:1320-1326.
23. Kimlin L.C., Casagrande G., Virador V.M. Invitro three-dimensional (3D) models in cancer research: an update. Mol Carcinog 2013, 52:167-182.
24. Li Q., Chen C., Kapadia A., Zhou Q., Harper M.K., Schaack J., et al. 3D models of epithelial-mesenchymal transition in breast cancer metastasis. JBiomol Screen 2011, 16:141-154.
25. Thiery J.P. Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer 2002, 2:442-454.
26. Lee J.M., Dedhar S., Kalluri R., Thompson E.W. The epithelial-mesenchymal transition: new insights in signaling, development, and disease. JCell Biol 2006, 172:973-981.
27. Dave B., Mittal V., Tan N., Chang J. Epithelial-mesenchymal transition, cancer stem cells and treatment resistance. Breast Cancer Res 2012, 14:1-5.
28. Zavadil J., Haley J., Kalluri R., Muthuswamy S.K., Thompson E. Epithelial-mesenchymal transition. Cancer Res 2008, 68:9574-9577.
29. Mendez M.G., Kojima S.-I., Goldman R.D. Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition. FASEB J 2010, 24:1838-1851.
30. Kuo C.-T., Chiang C.-L., Chang C.-H., Liu H.-K., Huang G.-S., Huang R.Y.-J., et al. Modeling of cancer metastasis and drug resistance via biomimetic nano-cilia and microfluidics. Biomaterials 2014, 35:1562-1571.
31. Donnenberg V.S., Landreneau R.J., Pfeifer M.E., Donnenberg A.D. Flow cytometric determination of stem/progenitor content in epithelial tissues: an example from nonsmall lung cancer and normal lung. Cytom Part A 2013, 83A:141-149.
32. Ho W.Y., Yeap S.K., Ho C.L., Rahim R.A., Alitheen N.B. Development of multicellular tumor spheroid (MCTS) culture from breast cancer cell and a high throughput screening method using the MTT assay. PLoS ONE 2012, 7:e44640.
33. Nicholson K.M., Bibby M.C., Phillips R.M. Influence of drug exposure parameters on the activity of paclitaxel in multicellular spheroids. Eur J Cancer 1997, 33:1291-1298.
34. Breslin S., O'Driscoll L. Three-dimensional cell culture: the missing link in drug discovery. Drug Discov Today 2013, 18:240-249.