Development of Multicellular Tumor Spheroid (MCTS) Culture from Breast Cancer Cell and a High Throughput Screening Method Using the MTT Assay

PLoS ONE

Volumn 7, Issue 9, 2012, Pages

  Ho, Wan Yong ^a   Yeap, Swee Keong ^a   Ho, Chai Ling ^a   Rahim, Raha Abdul ^a   Alitheen, Noorjahan Banu ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

[No Author keywords available]

Indexed keywords

LIPOCORTIN 5; TAMOXIFEN;

APOPTOSIS; ARTICLE; BREAST CANCER; CANCER CELL; CELL CULTURE; CELL VIABILITY; CLINICAL PROTOCOL; CYTOTOXICITY; FLOW CYTOMETRY; HIGH THROUGHPUT SCREENING; HUMAN; MICROSCOPY; SCANNING ELECTRON MICROSCOPY; SOLID TUMOR; TUMOR SPHEROID;

ANTINEOPLASTIC AGENTS, HORMONAL; BREAST NEOPLASMS; CELL DEATH; FEMALE; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; L-LACTATE DEHYDROGENASE; MCF-7 CELLS; MICROSCOPY, ELECTRON, SCANNING; TAMOXIFEN;

EID: 84866084556     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0044640     Document Type: Article

References (25)

1. Khaitan D, Chandna S, Arya MB, Dwarakanath BS, (2006) Establishment and characterization of multicellular spheroids from a human glioma cell line; Implications for tumor therapy. Journal of Translational Medicine 4: 12.
2. Herrmann R, Fayad W, Schwarz S, Berndtsson M, Linder S, (2008) Screening for compounds that induce apoptosis of cancer cells grown as multicellular spheroids. Journal of Biomolecular Screening 13: 1-8.
3. Dufau I, Frongia C, Sicard F, Dedieu L, Cordelier P, et al. (2012) Multicellular tumor spheroid model to evaluate spatio-temporal dynamics effect of chemotherapeutics: application to the gemcitabine/CHK1 inhibitor combination in pancreatic cancer. BMC Cancer 12: 15.
4. Kelm JM, Timmins NE, Brown CJ, Fussenegger M, Nielsen LK, (2003) Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnology and Bioengineering 83: 173-180.
5. Kim J, Stein R, O'Hare M, (2004) Three-dimensional in vitro tissue culture models of breast cancer - a review. Breast Cancer Research and Treatment 85: 281-291.
6. Onozuka H, Tsuchihara K, Esumi H, (2011) Hypoglycemic/hypoxic condition in vitro mimicking the tumor microenvironment markedly reduced the efficacy of anticancer drugs. Cancer Science 102: 975-982.
7. Minchinton AI, Tannock IF, (2006) Drug penetration in solid tumours. Nature Reviews Cancer 6: 583-592.
8. Ivascu A, Kubbies M, (2006) Rapid generation of single-tumor spheroids for high-throughput cell function and toxicity analysis. Journal of Biomolecular Screening 11: 922-932.
9. Lee J, Cuddihy MJ, Cater GM, Kotov NA, (2009) Engineering liver tissue spheroids with inverted colloidal crystal scaffolds. Biomaterials 30: 4687-4694.
10. Kunz-Schughart LA, Freyer JP, Hofstaedter F, Ebner R, (2004) The use of 3-D cultures for high-throughput screening: The multicellular spheroid model. Journal of Biomolecular Screening 9: 273-285.
11. Mosmann T, (1983) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65: 55-63.
12. Scherließ R, (2011) The MTT assay as tool to evaluate and compare excipient toxicity in vitro on respiratory epithelial cells. International Journal of Pharmaceutics 411: 98-105.
13. Jin C, Liang Y-J, He H, Fu L (2010) Synthesis and antitumor activity of novel chalcone derivatives. Biomedicine & Pharmacotherapy.
14. Baron M, Purcell W, Jackson S, Owen S, Jha A (2012) Towards a more representative in vitro method for fish ecotoxicology: morphological and biochemical characterisation of three-dimensional spheroidal hepatocytes. Ecotoxicology: 1-11.
15. Howes AL, Chiang GG, Lang ES, Ho CB, Powis G, et al. (2007) The phosphatidylinositol 3-kinase inhibitor, PX-866, is a potent inhibitor of cancer cell motility and growth in three-dimensional cultures. Molecular Cancer Therapeutics 6: 2505-2514.
16. Lam JT, Hemminki A, Kanerva A, Lee KB, Blackwell JL, et al. (2007) A three-dimensional assay for measurement of viral-induced oncolysis. Cancer Gene Ther 14: 421-430.
17. Ho WY, Yeap SK, Ho CL, Raha AR, Suraini AA, et al. (2011) Elephantopus scaber induces cytotoxicity in MCF-7 human breast cancer cells via p53-induced apoptosis. Journal of Medicinal Plants Research 5: 5741-5749.
18. Braidy N, Grant R, Brew BJ, Adams S, Jayasena T, et al. (2009) Guillemin effects of kynurenine pathway metabolites on intracellular NAD+ synthesis and cell death in human primary astrocytes and neurons. International Journal of Tryptophan Research 2 61-69.
19. Madsen SJ, Sun CH, Tromberg BJ, Cristini V, De Magalhaes N, et al. (2006) Multicell tumor spheroids in photodynamic therapy. Lasers in Surgery and Medicine 38: 555-564.
20. Sutherland R, (1988) Cell and environment interactions in tumor microregions: the multicell spheroid model. Science 240: 177-184.
21. Ciurana CLF, Zwart B, van Mierlo G, Hack CE, (2004) Complement activation by necrotic cells in normal plasma environment compares to that by late apoptotic cells and involves predominantly IgM. European Journal of Immunology 34: 2609-2619.
22. Nigro P, Bloise E, Turco MC, Skhirtladze A, Montoro P, et al. (2007) Antiproliferative and pro-apoptotic activity of novel phenolic derivatives of resveratrol. Life Sciences 81: 873-883.
23. Fracasso G, Colombatti M, (2000) Effect of therapeutic macromolecules in spheroids. Critical Reviews in Oncology/Hematology 36: 159-178.
24. Mueller-Klieser W, (2000) Tumor biology and experimental therapeutics. Critical reviews in oncology/hematology 36: 123-139.
25. Xiao Y, Li JD, Shi HL, Liu JH, Feng YL, et al. (2007) Predictive value of in vitro MTT assay chemosensitivity test of cytotoxic drug activity in cervical cancer. Ai Zheng 26: 386-389.