Purification and visualization of encephalomyocarditisvirus synthesized by an in vitro protein expression system derived from mammalian cell extract

Biotechnology Letters

Volumn 35, Issue 3, 2013, Pages 309-314

  Kobayashi, Tominari ^a   Yukigai, Jun ^a   Ueda, Kosaku ^a   Machida, Kodai ^a   Masutani, Mamiko ^a   Nishino, Yuri ^a,c   Miyazawa, Atsuo ^a,c   Imataka, Hiroaki ^a,b  

^a UNIVERSITY OF HYOGO   (Japan)

^b RIKEN   (Japan)

^c Harima Institute ^*  (Japan)

Author keywords

Cell free synthesis; Encephalomyocarditis virus; RNA virus; Transmission electron microscopy; Virus particles; Virus synthesis

Indexed keywords

CYTOLOGY; DRUG DELIVERY; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; LANTHANUM COMPOUNDS; MAMMALS; PROTEINS; PURIFICATION; RNA; TRANSMISSION ELECTRON MICROSCOPY; TRANSMISSIONS; VIRUSES;

CELL FREE SYNTHESIS; CELL-FREE PROTEIN EXPRESSION; ENCEPHALOMYOCARDITIS VIRUS; MATERIAL SCIENCE; PROTEIN EXPRESSION SYSTEMS; RNA VIRUS; SYNTHESIZED PARTICLES; VIRUS PARTICLES;

BIOSYNTHESIS;

ENCEPHALOMYOCARDITIS VIRUS; MAMMALIA; RNA VIRUSES;

EID: 84873995751     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-012-1086-1     Document Type: Article

References (16)

1. Arnold E, Luo M, Vriend G, Rossmann MG, Palmenberg AC, Parks GD, Nicklin MJ, Wimmer E (1987) Implications of the picornavirus capsid structure for polyprotein processing. Proc Natl Acad Sci USA 84: 21-25.
2. Barton DJ, O'Donnell BJ, Flanegan JB (2001) 5′ cloverleaf in poliovirus RNA is a cis-acting replication element required for negative-strand synthesis. EMBO J 20: 1439-1448.
3. Herold J, Andino R (2000) Poliovirus requires a precise 5′ end for efficient positive-strand RNA synthesis. J Virol 74: 6394-6400.
4. Kitai Y, Fukuda H, Enomoto T, Asakawa Y, Suzuki T, Inouye S, Handa H (2011) Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor. J Biotechnol 155: 251-256.
5. Kobayashi T, Mikami S, Yokoyama S, Imataka H (2007) An improved cell-free system for picornavirus synthesis. J Virol Methods 142: 182-188.
6. Kobayashi T, Machida K, Mikami S, Masutani M, Imataka H (2011) Cell-free RNA replication systems based on a human cell extracts-derived in vitro translation system with the encephalomyocarditisvirus RNA. J Biochem 150: 423-430.
7. Kobayashi T, Nakamura Y, Mikami S, Masutani M, Machida K, Imataka H (2012) Synthesis of encephalomyocarditis virus in a cell-free system: from DNA to RNA virus in one tube. Biotechnol Lett 34: 67-73.
8. Ludwig C, Wagner R (2007) Virus-like particles-universal molecular toolboxes. Curr Opin Biotechnol 18: 537-545.
9. Machida K, Masutani M, Kobayashi T, Mikami S, Nishino Y, Miyazawa A, Imataka H (2012) Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells. Protein Expr Purif 82: 61-69.
10. Mikami S, Kobayashi T, Yokoyama S, Imataka H (2006a) A hybridoma-based in vitro translation system that efficiently synthesizes glycoproteins. J Biotechnol 127: 65-78.
11. Mikami S, Masutani M, Sonenberg N, Yokoyama S, Imataka H (2006b) An efficient mammalian cell-free translation system supplemented with translation factors. Protein Expr Purif 46: 348-357.
12. Mikami S, Kobayashi T, Machida K, Masutani M, Yokoyama S, Imataka H (2010) N-terminally truncated GADD34 proteins are convenient translation enhancers in a human cell-derived in vitro protein synthesis system. Biotechnol Lett 32: 897-902.
13. Palmenberg AC (1990) Proteolytic processing of picornaviral polyprotein. Annu Rev Microbiol 44: 603-623.
14. Pestova TV, Hellen CU, Shatsky IN (1996) Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry. Mol Cell Biol 16: 6859-6869.
15. Strable E, Finn MG (2009) Chemical modification of viruses and virus-like particles. Curr Top Microbiol Immunol 327: 1-21.
16. Svitkin YV, Sonenberg N (2003) Cell-free synthesis of encephalomyocarditis virus. J Virol 77: 6551-6555.