An efficient mammalian cell-free translation system supplemented with translation factors

Protein Expression and Purification

Volumn 46, Issue 2, 2006, Pages 348-357

  Mikami, Satoshi ^a   Masutani, Mamiko ^a   Sonenberg, Nahum ^d   Yokoyama, Shigeyuki ^a,b,c   Imataka, Hiroaki ^a  

^a RIKEN YOKOHAMA INSTITUTE   (Japan)

^b RIKEN HARIMA INSTITUTE   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

^d MCGILL UNIVERSITY   (Canada)

Author keywords

Cell free; Eukaryotic translation initiation factor; HeLa

Indexed keywords

CAPPED RNA; INITIATION FACTOR; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; CELL FREE SYSTEM; COMPARATIVE STUDY; HELA CELL; HUMAN; PROTEIN SYNTHESIS;

CELL-FREE SYSTEM; HELA CELLS; HUMANS; PROKARYOTIC INITIATION FACTORS; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; RNA CAPS;

ENCEPHALOMYOCARDITIS VIRUS; EUKARYOTA; MAMMALIA; ORYCTOLAGUS CUNICULUS;

EID: 33645395535     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.09.021     Document Type: Article

References (40)

1. B. Raught, A.C. Gingeras, and N. Sonenberg Regulation of Ribosomal Recruitment in Eukaryotes 2000 Cold Spring Harbor Laboratory Cold Spring Harbor, NY
2. A. Hinnebusch Mechanism and Regulation of Initiator Methionyl-tRNA Binding to Ribosomes 2000 Cold Spring Harbor Laboratory Cold Spring Harbor, NY
3. A.C. Gingras, B. Raught, and N. Sonenberg mTOR signaling to translation Curr. Top. Microbiol. Immunol. 279 2004 169 197
4. A.G. Rowlands, R. Panniers, and E.C. Henshaw The catalytic mechanism of guanine nucleotide exchange factor action and competitive inhibition by phosphorylated eukaryotic initiation factor 2 J. Biol. Chem. 263 1988 5526 5533
5. H.P. Harding, M. Calfon, F. Urano, I. Novoa, and D. Ron Transcriptional and translational control in the Mammalian unfolded protein response Annu. Rev. Cell Dev. Biol. 18 2002 575 599
6. R.J. Kaufman Regulation of mRNA translation by protein folding in the endoplasmic reticulum Trends Biochem. Sci. 29 2004 152 158
7. N. Iizuka, L. Najita, A. Franzusoff, and P. Sarnow Cap-dependent and cap-independent translation by internal initiation of mRNAs in cell extracts prepared from Saccharomyces cerevisiae Mol. Cell Biol. 14 1994 7322 7330
8. G. Bergamini, T. Preiss, and M.W. Hentze Picornavirus IRESes and the poly(A) tail jointly promote cap-independent translation in a mammalian cell-free system RNA 6 2000 1781 1790
9. Y.V. Svitkin, H. Imataka, K. Khaleghpour, A. Kahvejian, H.D. Liebig, and N. Sonenberg Poly(A)-binding protein interaction with elF4G stimulates picornavirus IRES-dependent translation RNA 7 2001 1743 1752
10. F. Gebauer, D.F. Corona, T. Preiss, P.B. Becker, and M.W. Hentze Translational control of dosage compensation in Drosophila by sex-lethal: cooperative silencing via the 5′ and 3′ UTRs of msl-2 mRNA is independent of the poly(A) tail EMBO J. 18 1999 6146 6154
11. T. Kigawa, T. Yabuki, Y. Yoshida, M. Tsutsui, Y. Ito, T. Shibata, and S. Yokoyama Cell-free production and stable-isotope labeling of milligram quantities of proteins FEBS Lett. 442 1999 15 19
12. K. Madin, T. Sawasaki, T. Ogasawara, and Y. Endo A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes Proc. Natl. Acad. Sci. USA 97 2000 559 564
13. K.A. Underwood, J.R. Swartz, and J.D. Puglisi Quantitative polysome analysis identifies limitations in bacterial cell-free protein synthesis Biotechnol. Bioeng. 91 2005 425 435
14. T. Sawasaki, Y. Hasegawa, M. Tsuchimochi, N. Kamura, T. Ogasawara, T. Kuroita, and Y. Endo A bilayer cell-free protein synthesis system for high-throughput screening of gene products FEBS Lett. 514 2002 102 105
15. Y.V. Svitkin, L.P. Ovchinnikov, G. Dreyfuss, and N. Sonenberg General RNA binding proteins render translation cap dependent EMBO J. 15 1996 7147 7155
16. H. Imataka, A. Gradi, and N. Sonenberg A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A)-binding protein and functions in poly(A)-dependent translation EMBO J. 17 1998 7480 7489
17. S. Morino, H. Imataka, Y.V. Svitkin, T.V. Pestova, and N. Sonenberg Eukaryotic translation initiation factor 4E (eIF4E) binding site and the middle one-third of eIF4GI constitute the core domain for cap-dependent translation, and the C-terminal one-third functions as a modulatory region Mol. Cell Biol. 20 2000 468 477
18. H. Imataka, H.S. Olsen, and N. Sonenberg A new translational regulator with homology to eukaryotic translation initiation factor 4G EMBO J. 16 1997 817 825
19. M. Jaramillo, J. Pelletier, I. Edery, P.J. Nielsen, and N. Sonenberg Multiple mRNAs encode the murine translation initiation factor eIF-4E J. Biol. Chem. 266 1991 10446 10451
20. A. Pause, and N. Sonenberg Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF-4A EMBO J. 11 1992 2643 2654
21. H. Trachsel, B. Erni, M.H. Schreier, L. Braun, and T. Staehelin Purification of seven protein synthesis initiation factors from Krebs II ascites cells Biochim. Biophys. Acta 561 1979 484 490
22. C. Thoma, A. Ostareck-Lederer, and M.W. Hentze A poly(A) tail-responsive in vitro system for cap- or IRES-driven translation from HeLa cells Methods Mol. Biol. 257 2004 171 180
23. L.P. Ford, P.S. Bagga, and J. Wilusz The poly(A) tail inhibits the assembly of a 3′-to-5′ exonuclease in an in vitro RNA stability system Mol. Cell Biol. 17 1997 398 406
24. T. von der Haar, and J.E. McCarthy Intracellular translation initiation factor levels in Saccharomyces cerevisiae and their role in cap-complex function Mol. Microbiol. 46 2002 531 544
25. R. Duncan, S.C. Milburn, and J.W. Hershey Regulated phosphorylation and low abundance of HeLa cell initiation factor eIF-4F suggest a role in translational control. Heat shock effects on eIF-4F J. Biol. Chem. 262 1987 380 388
26. M. Rau, T. Ohlmann, S.J. Morley, and V.M. Pain A reevaluation of the cap-binding protein, eIF4E, as a rate-limiting factor for initiation of translation in reticulocyte lysate J. Biol. Chem. 271 1996 8983 8990
27. A. Pause, G.J. Belsham, A.C. Gingras, O. Donze, T.A. Lin, J.C. Lawrence Jr., and N. Sonenberg Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5′-cap function Nature 371 1994 762 767
28. V.I. Baranov, I. Morozov, S.A. Ortlepp, and A.S. Spirin Gene expression in a cell-free system on the preparative scale Gene 84 1989 463 466
29. G.D. Pavitt, K.V. Ramaiah, S.R. Kimball, and A.G. Hinnebusch eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange Genes Dev. 12 1998 514 526
30. T.V. Pestova, C.U. Hellen, and I.N. Shatsky Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry Mol. Cell Biol. 16 1996 6859 6869
31. T.V. Pestova, S.I. Borukhov, and C.U. Hellen Eukaryotic ribosomes require initiation factors 1 and 1A to locate initiation codons Nature 394 1998 854 859
32. T.V. Pestova, I.B. Lomakin, J.H. Lee, S.K. Choi, T.E. Dever, and C.U. Hellen The joining of ribosomal subunits in eukaryotes requires eIF5B Nature 403 2000 332 335
33. S. Oldfield, B.L. Jones, D. Tanton, and C.G. Proud Use of monoclonal antibodies to study the structure and function of eukaryotic protein synthesis initiation factor eIF-2B Eur. J. Biochem. 221 1994 399 410
34. J. Marcotrigiano, A.C. Gingras, N. Sonenberg, and S.K. Burley Cocrystal structure of the messenger RNA 5′ cap-binding protein (eIF4E) bound to 7-methyl-GDP Cell 89 1997 951 961
35. A. Lazaris-Karatzas, K.S. Montine, and N. Sonenberg Malignant transformation by a eukaryotic initiation factor subunit that binds to mRNA 5′ cap Nature 345 1990 544 547
36. H.G. Wendel, E. De Stanchina, J.S. Fridman, A. Malina, S. Ray, S. Kogan, C. Cordon-Cardo, J. Pelletier, and S.W. Lowe Survival signalling by Akt and eIF4E in oncogenesis and cancer therapy Nature 428 2004 332 337
37. D. Ruggero, L. Montanaro, L. Ma, W. Xu, P. Londei, C. Cordon-Cardo, and P.P. Pandolfi The translation factor eIF-4E promotes tumor formation and cooperates with c-Myc in lymphomagenesis Nat. Med. 10 2004 484 486
38. D. Rousseau, A.C. Gingras, A. Pause, and N. Sonenberg The eIF4E-binding proteins 1 and 2 are negative regulators of cell growth Oncogene 13 1996 2415 2420
39. Y. Furuichi, A. LaFiandra, and A.J. Shatkin 5′-Terminal structure and mRNA stability Nature 266 1977 235 239
40. R. Parker, and H. Song The enzymes and control of eukaryotic mRNA turnover Nat. Struct. Mol. Biol. 11 2004 121 127