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Volumn 1344, Issue , 2014, Pages 59-65
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Integrative refolding and purification of histidine-tagged protein by like-charge facilitated refolding and metal-chelate affinity adsorption
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Author keywords
Affinity adsorption; Inclusion bodies; Like charge; Metal chelate; Protein refolding; Purification
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Indexed keywords
ADSORPTION;
AGGLOMERATION;
AMINO ACIDS;
CHELATION;
DYES;
ESCHERICHIA COLI;
IMPURITIES;
METAL RECOVERY;
METALS;
PURIFICATION;
RESINS;
UREA;
AFFINITY ADSORPTION;
INCLUSION BODIES;
LIKE CHARGES;
METAL CHELATE;
PROTEIN REFOLDING;
PROTEINS;
DIGLYCINE;
ENHANCED GREEN FLUORESCENT PROTEIN;
HEXAHISTIDINE;
METAL CHELATE;
NICKEL;
RESIN;
SEPHAROSE;
UREA;
ADSORPTION;
AFFINITY ADSORPTION;
ARTICLE;
CELL INCLUSION;
CONTROLLED STUDY;
DILUTION;
ESCHERICHIA COLI;
NONHUMAN;
PRIORITY JOURNAL;
PROTEIN BINDING;
PROTEIN EXPRESSION;
PROTEIN PURIFICATION;
PROTEIN REFOLDING;
SOLUBILIZATION;
SURFACE CHARGE;
AFFINITY ADSORPTION;
INCLUSION BODIES;
LIKE-CHARGE;
METAL CHELATE;
PROTEIN REFOLDING;
PURIFICATION;
ADSORPTION;
CHELATING AGENTS;
CHROMATOGRAPHY, AFFINITY;
COORDINATION COMPLEXES;
COPPER;
GELS;
GREEN FLUORESCENT PROTEINS;
HISTIDINE;
IMINO ACIDS;
METALS;
NICKEL;
OLIGOPEPTIDES;
PROTEIN REFOLDING;
PROTEINS;
RECOMBINANT PROTEINS;
RESINS, SYNTHETIC;
SEPHAROSE;
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EID: 84899917560
PISSN: 00219673
EISSN: 18733778
Source Type: Journal
DOI: 10.1016/j.chroma.2014.04.006 Document Type: Article |
Times cited : (12)
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References (38)
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