Ion-exchange resins greatly facilitate refolding of like-charged proteins at high concentrations

Biotechnology and Bioengineering

Volumn 108, Issue 5, 2011, Pages 1068-1077

  Wang, Guo Zhen ^a   Dong, Xiao Yan ^a   Sun, Yan ^a  

^a TIANJIN UNIVERSITY   (China)

Author keywords

Aggregation; Electrostatic repulsion; Ion exchange resin; Like charge; Protein refolding

Indexed keywords

AGGREGATION; BOVINE SERUM ALBUMINS; CHARGE REPULSION; CHARGED IMPURITY; CHARGED PROTEINS; CHARGED SURFACES; ELECTROSTATIC REPULSION; ENHANCING EFFECT; FOLDING INTERMEDIATES; FOLDING KINETICS; FOLDING PROCESS; GEL BEADS; HIGH CONCENTRATION; HIGH EFFICIENCY; IN-VITRO; INCLUSION BODIES; KEY MATERIALS; LIKE CHARGE; MOLECULAR CROWDING; NATIVE PROTEINS; POSITIVELY CHARGED; PROTEIN AGGREGATION; PROTEIN CONCENTRATIONS; PROTEIN MOLECULES; PROTEIN REFOLDING; REFOLDING; THERAPEUTIC PROTEIN; WORKING MECHANISMS;

ACTINIDES; ADSORPTION; AGGLOMERATION; BACTERIOLOGY; BODY FLUIDS; CHARGED PARTICLES; ELECTROSTATICS; GELS; ION EXCHANGE RESINS; POSITIVE IONS; PROTEIN FOLDING;

ION EXCHANGE;

ADSORBENT; ALBUMIN; BOVINE SERUM ALBUMIN; CARBONATE DEHYDRATASE II; CYTIDINE 2',3' CYCLIC PHOSPHATE; CYTIDINE DERIVATIVE; DITHIOTHREITOL; LYSOZYME; PROTEIN; RESIN; RIBONUCLEASE A; TROMETAMOL; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION (PARAMETERS); GEL; ION EXCHANGE; MICROCOCCUS LUTEUS; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; STATIC ELECTRICITY;

ION EXCHANGE RESINS; PROTEIN FOLDING; PROTEINS; STATIC ELECTRICITY;

BOVINAE;

EID: 79953149898     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23038     Document Type: Article

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