Fluorescence of a histidine-modified enhanced green fluorescent protein (EGFP) effectively quenched by copper(II) ions

Journal of Fluorescence

Volumn 23, Issue 2, 2013, Pages 273-281

  Bálint, Emese Éva ^a,b   Petres, Judit ^a   Szabó, Mária ^b   Orbán, Csongor Kálmán ^a,b   Szilágyi, László ^b,c   Ábrahám, Beáta ^b  

^a UNIVERSITY POLITEHNICA OF BUCHAREST   (Romania)

^b SAPIENTIA HUNGARIAN UNIVERSITY OF TRANSYLVANIA   (Romania)

^c EÖTVÖS LORÁND UNIVERSITY   (Hungary)

Author keywords

Copper(II); EGFP; Fluorescence quenching,; Mutagenesis; pH; Temperature

Indexed keywords

CHROMOPHORE GROUP; EGFP; ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENCE QUENCHING; FLUORESCENCE SPECTRA; METAL BINDING SITES; OPTIMAL CONDITIONS; SITE DIRECTED MUTAGENESIS;

CHROMOPHORES; COPPER; FLUORESCENCE; FLUOROPHORES; METAL IONS; MUTAGENESIS; PH; PROTEINS; QUENCHING; TEMPERATURE;

AMINO ACIDS;

COPPER; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HISTIDINE;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; METHODOLOGY; PH; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; SITE DIRECTED MUTAGENESIS; SPECTROFLUOROMETRY; TEMPERATURE;

COPPER; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GREEN FLUORESCENT PROTEINS; HISTIDINE; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; SPECTROMETRY, FLUORESCENCE; TEMPERATURE;

EID: 84879679905     PISSN: 10530509     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10895-012-1145-y     Document Type: Article

References (39)

1. Tapiero H, Townsend DM, Tew KD (2003) Trace elements in human physiology and pathology. Copper. Biomed Pharmacother 57(9):386-98
2. Kramer ML et al (2001) Prion protein binds copper within the physiological concentration range. J Biol Chem 276(20):16711-9
3. Squitti R et al (2011) Copper status in Alzheimer's disease and other neurodegenerative disorders: genetics, mechanisms, neurophysiology, and therapies. Int J Alzheimers Dis 903-940
4. Urbanski NK, Beresewicz A (2000) Generation of *OH initiated by interaction of Fe2+ and Cu + with dioxygen; comparison with the Fenton chemistry. Acta Biochim Pol 47(4):951-62
5. Arnal N, de Alaniz MJT, Marra CA (2012) Cytotoxic effects of copper overload on human-derived lung and liver cells in culture. Biochim Biophys Acta (BBA) - General Subjects 1820(7):931-939
6. Huang GG, Yang J (2003) Selective detection of copper ions in aqueous solution based on an evanescent wave infrared absorption spectroscopic method. Anal Chem 75(10):2262-9
7. Lin M et al (2009) Electrochemical detection of copper ion using a modified copolythiophene electrode. Electrochim Acta 54(27):7012-7017
8. Yang M et al (2010) Detection of copper ion with laser-induced fluorescence in a capillary electrophoresis microchip. Anal Lett 43(18):2883-2891
9. Liu SR, Wu SP (2011) An NBD-based sensitive and selective fluorescent sensor for copper(II) ion. J Fluoresc 21(4):1599-605
10. Tang R et al (2012) A rhodamine-based off-on fluorescent chemosensor for selectively sensing Cu(II) in aqueous solution. J Fluoresc 21(1):141-8
11. Wang Y et al (2012) A novel chemosensor based on rhodamine derivative for colorimetric and fluorometric detection of Cu2+ in aqueous solution. J Fluoresc 22(3):799-805
12. Yildirim M, Kaya I (2010) Synthesis of a novel fluorescent Schiff base as a possible Cu(II) ion selective sensor. J Fluoresc 20(3):771-7
13. Tsien RY (1998) The green fluorescent protein. Annu Rev Biochem 67:509-44
14. Shimomura O (2005) The discovery of aequorin and green fluorescent protein. J Microsc 217(Pt 1):1-15
15. Prasher DC et al (1992) Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111(2):229-33
16. Chalfie M et al (1994) Green fluorescent protein as a marker for gene expression. Science 263(5148):802-5
17. Zimmer M (2002) Green fluorescent protein (GFP): applications, structure, and related photophysical behavior. Chem Rev 102(3):759-81
18. Bizzarri R et al (2007) Green fluorescent protein ground states: the influence of a second protonation site near the chromophore. Biochemistry 46(18):5494-504
19. Heim R, Cubitt AB, Tsien RY (1995) Improved green fluorescence. Nature 373(6516):663-4
20. Heim R, Tsien RY (1996) Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol 6(2):178-82
21. Tansila N et al (2008) Metal ion accessibility of histidine-modified superfolder green fluorescent protein expressed in Escherichia coli. Biotechnol Lett 30(8):1391-6
22. Palfi M, Kovacs E, Miklossy I, Szilagyi L, Abraham B, Lanyi Sz (2009) Engineered Green Fluorescent Protein as a Potential Metal Sensor. Studia Universitatis Babes-Bolyai Chemia (Special Issue): 35-45
23. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680-685
24. Ellis KJ, Morrison JF (1982) Buffers of constant ionic strength for studying pH-dependent processes. Methods Enzymol 87:405-26
25. Williams AT, Winfield SA, Miller JN (1983) Relative fluorescence quantum yields using a computer controlled luminescence spectrometer. Analyst 108:1067-107
26. Brannon JM, Magde D (1978) Absolute quantum yield determination by thermal blooming: fluorescein. J Phys Chem 82(6):705-709
27. Green NM (1965) A spectrophotometric assay for avidin and biotin based on binding of dyes by avidin. Biochem J 94:23C-24C
28. Schrodinger LLC (2010) The PyMOL molecular graphics system, Version 1.3r1
29. Palm GJ et al (1997) The structural basis for spectral variations in green fluorescent protein. Nat Struct Biol 4(5):361-5
30. Kneen M et al (1998) Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys J 74(3):1591-9
31. Patterson GH et al (1997) Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophys J 73(5):2782-90
32. Matz MV et al (1999) Fluorescent proteins from nonbioluminescent Anthozoa species. Nat Biotechnol 17(10):969-73
33. Eli P, Chakrabartty A (2006) Variants of DsRed fluorescent protein: development of a copper sensor. Protein Sci 15(10):2442-7
34. Rahimi Y et al (2008) Mechanism of copper induced fluorescence quenching of red fluorescent protein, DsRed. Biochem Biophys Res Commun 370(1):57-61
35. Sumner JP et al (2006) DsRed as a highly sensitive, selective, and reversible fluorescence-based biosensor for both Cu(+) and Cu(2+) ions. Biosens Bioelectron 21(7):1302-8
36. Richmond TA et al (2000) Engineered metal binding sites on green fluorescence protein. Biochem Biophys Res Commun 268(2):462-5
37. Hotzer B et al (2011) Determination of copper(II) ion concentration by lifetime measurements of green fluorescent protein. J Fluoresc 21(6):2143-53
38. Tang S et al (2011) Design and application of a class of sensors to monitor Ca2+ dynamics in high Ca2+ concentration cellular compartments. Proc Natl Acad Sci USA 108(39):16265-70
39. Wang ZX, Jiang RF (1996) A novel two-site binding equation presented in terms of the total ligand concentration. FEBS Lett 392(3):245-9 Bioengineering