Purification and on-column refolding of EGFP overexpressed as inclusion bodies in Escherichia coli with expanded bed anion exchange chromatography

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 818, Issue 1 SPEC. ISS., 2005, Pages 23-27

  Cabanne, C ^a   Noubhani, A M ^a   Hocquellet, A ^a   Dole, F ^a   Dieryck, W ^a   Santarelli, X ^a  

^a UNIVERSITÉ DE BORDEAUX   (France)

Author keywords

Chromatography; EGFP; Expanded bed adsorption; Inclusion bodies; Purification; Refolding

Indexed keywords

ADSORPTION; CENTRIFUGATION; CHROMATOGRAPHY; ELECTROPHORESIS; ESCHERICHIA COLI; FLUORESCENCE; ION EXCHANGE; NEGATIVE IONS; PURIFICATION; SOLUBILITY;

COLUMN REFOLDING; ENHANCED GREEN FLUORESCENT PROTEIN (EGFP); EXPANDED BED ADSORPTION (EBA); INCLUSION BODIES;

PROTEINS;

ENHANCED GREEN FLUORESCENT PROTEIN; SODIUM CHLORIDE; UREA;

ANION EXCHANGE CHROMATOGRAPHY; CELL INCLUSION; CENTRIFUGATION; CONFERENCE PAPER; CONTROLLED STUDY; ELECTROPHORESIS; ELUTION; ESCHERICHIA COLI; EXPANDED BED CHROMATOGRAPHY; FLUORESCENCE; GENE OVEREXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION;

ESCHERICHIA COLI;

EID: 13844297856     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2004.10.023     Document Type: Conference Paper

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