Refolding of recombinant homodimeric malate dehydrogenase expressed in Escherichia coli as inclusion bodies

Biochemical Engineering Journal

Volumn 38, Issue 3, 2008, Pages 341-348

  Dong, Xiao Yan ^a   Fu, Min Ling ^a   Sun, Yan ^a  

^a TIANJIN UNIVERSITY   (China)

Author keywords

Artificial chaperone; Expression; Folding aids; Inclusion bodies; Malate dehydrogenase; Refolding

Indexed keywords

ADENOSINETRIPHOSPHATE; CATALYST ACTIVITY; DICHROISM; DIMERS; ENZYME ACTIVITY;

ARTIFICIAL CHAPERONES; HOMODIMERIC ENZYMES; INCLUSION BODIES (IBS); MALATE DEHYDROGENASE;

ESCHERICHIA COLI;

MALATE DEHYDROGENASE;

ALPHA HELIX; ARTICLE; CATALYSIS; CELL INCLUSION; CIRCULAR DICHROISM; DIMERIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBUNIT; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; GEL PERMEATION CHROMATOGRAPHY; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LOW TEMPERATURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN TERTIARY STRUCTURE;

ESCHERICHIA COLI;

EID: 38849136644     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2007.07.022     Document Type: Article

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